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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
01/10/2019 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BÁEZ, F.; CAMARGO, A.A.; GASTAL, G.D.A. |
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Afiliación : |
FRANCISCO BÁEZ, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; ÁLVARO A. CAMARGO, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Ultrastructural imaging analysis of the zona pellucida surface in bovine oocytes. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Microscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036. |
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DOI : |
10.1017/S1431927619000692 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019. |
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Contenido : |
Abstract:
The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and
mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes
(COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development. |
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Palabras claves : |
BOVINE; IN VITRO MATURATION; PLATAFORMA SALUD ANIMAL; ULTRASTRUCTURE; ZONA PELLUCIDA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02045naa a2200229 a 4500
001 1060255
005 2019-10-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1017/S1431927619000692$2DOI
100 1 $aBÁEZ, F.
245 $aUltrastructural imaging analysis of the zona pellucida surface in bovine oocytes.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019.
520 $aAbstract: The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes (COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development.
653 $aBOVINE
653 $aIN VITRO MATURATION
653 $aPLATAFORMA SALUD ANIMAL
653 $aULTRASTRUCTURE
653 $aZONA PELLUCIDA
700 1 $aCAMARGO, A.A.
700 1 $aGASTAL, G.D.A.
773 $tMicroscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
16/01/2015 |
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Actualizado : |
29/08/2016 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
ACOSTA, Y.; MIERES, J.; LA MANNA, A. |
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Afiliación : |
YAMANDU MARTIN ACOSTA AZPIROZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN MANUEL MIERES VISILLAC, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Sugerencias para el manejo de animales y recursos de alimentación en situación de eventual sequía. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
El Tambo, n. 179, p. 72-74,76-77, 2010. |
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Idioma : |
Español |
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Palabras claves : |
AFRECHILLO DE ARROZ; AFRECHILLO DE TRIGO; ALIMENTACIÓN ANIMAL; CÁSCARA DE SOJA; CASCARILLA DE SOJA; CONCENTRADOS (SUPLEMENTOS); EXPELER DE GIRASOL; EXPELLER DE SOJA; FORRAJES BASTOS; GRANO DE CEBADA; GRANO DE MAÍZ; GRANO DE SORGO; GRANO DE TRIGO; MANEJO DEL RODEO; MELAZA; MINERALES (SUPLEMENTOS); SEQUÍA; UREA. |
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Thesagro : |
LECHERÍA; PRODUCCIÓN ANIMAL. |
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Asunto categoría : |
L02 Alimentación animal |
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Marc : |
LEADER 01047naa a2200373 a 4500
001 1052051
005 2016-08-29
008 2010 bl uuuu u00u1 u #d
100 1 $aACOSTA, Y.
245 $aSugerencias para el manejo de animales y recursos de alimentación en situación de eventual sequía.
260 $c2010
650 $aLECHERÍA
650 $aPRODUCCIÓN ANIMAL
653 $aAFRECHILLO DE ARROZ
653 $aAFRECHILLO DE TRIGO
653 $aALIMENTACIÓN ANIMAL
653 $aCÁSCARA DE SOJA
653 $aCASCARILLA DE SOJA
653 $aCONCENTRADOS (SUPLEMENTOS)
653 $aEXPELER DE GIRASOL
653 $aEXPELLER DE SOJA
653 $aFORRAJES BASTOS
653 $aGRANO DE CEBADA
653 $aGRANO DE MAÍZ
653 $aGRANO DE SORGO
653 $aGRANO DE TRIGO
653 $aMANEJO DEL RODEO
653 $aMELAZA
653 $aMINERALES (SUPLEMENTOS)
653 $aSEQUÍA
653 $aUREA
700 1 $aMIERES, J.
700 1 $aLA MANNA, A.
773 $tEl Tambo$gn. 179, p. 72-74,76-77, 2010.
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