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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2016 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
VERDES, J. M.; MÁRQUEZ, M.; CALLIARI, A.; BATES, D.; MORAÑA, J. A.; GIMENO, E. J.; ODRIOZOLA, E.; GIANNITTI, F.; GUERRERO, F.; FIDALGO, L. E.; PUMAROLA, M. |
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Afiliación : |
JOSÉ MANUEL VERDES, Universidad de la República (UdelaR)/ Facultad de Veterinaria; MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MERCEDES MÁRQUEZ, Universidad de Santiago de Compostela (USC); ALDO CALLIARI, Universidad de la República (UdelaR)/ Facultad de Veterinaria; MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); DANIEL BATES, Universidad de la República (UdelaR)/ Facultad de Veterinaria; JOSÉ ANTONIO MORAÑA, Universidad de la República (UdelaR)/ Facultad de Veterinaria; EDUARDO JUAN GIMENO, Universidad Nacional de la Plata (UNLP); ERNESTO ODRIOZOLA, INTA (Instituto Nacional de Tecnología Agropecuaria)- Balcarce; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENTINA GUERRERO, Universidad de Santiago de Compostela (USC); LUIS EUSEBIO FIDALGO, Universidad de Santiago de Compostela (USC); MARTÍ PUMAROLA, Universitat Autònoma de Barcelona. |
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Título : |
A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle. (Full Scientific Report) |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, 2015, v.27 (3): 278-286. |
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DOI : |
10.1177/1040638715582048 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Published April 21, 2015. |
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Contenido : |
ABSTRACT.
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
© 2015 The Author(s). MenosABSTRACT.
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on th... Presentar Todo |
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Palabras claves : |
BOVINE; CEREBELLAR DEGENERATION; CYTOSKELETAL DYSFUNCTION; PURKINJE CELLS; SOLANUM BONARIENSE. |
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Thesagro : |
BOVINOS. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02961naa a2200337 a 4500
001 1056202
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638715582048$2DOI
100 1 $aVERDES, J. M.
245 $aA novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle. (Full Scientific Report)$h[electronic resource]
260 $c2015
500 $aArticle history: First Published April 21, 2015.
520 $aABSTRACT. Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death. © 2015 The Author(s).
650 $aBOVINOS
653 $aBOVINE
653 $aCEREBELLAR DEGENERATION
653 $aCYTOSKELETAL DYSFUNCTION
653 $aPURKINJE CELLS
653 $aSOLANUM BONARIENSE
700 1 $aMÁRQUEZ, M.
700 1 $aCALLIARI, A.
700 1 $aBATES, D.
700 1 $aMORAÑA, J. A.
700 1 $aGIMENO, E. J.
700 1 $aODRIOZOLA, E.
700 1 $aGIANNITTI, F.
700 1 $aGUERRERO, F.
700 1 $aFIDALGO, L. E.
700 1 $aPUMAROLA, M.
773 $tJournal of Veterinary Diagnostic Investigation, 2015$gv.27 (3): 278-286.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Treinta y Tres. |
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Fecha actual : |
22/09/2020 |
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Actualizado : |
23/09/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
VILLARINO, S.H.; PINTO, P.; DELLA CHIESA, T.; JOBBÁGY, E.G.; STUDDERT, G.A.; BAZZONI, B.; CONTI, G.; RUFINO, M.; ÁLVAREZ, R.; BODDEY , R.; BAYER, C.; DE F CARVALHO, P.C.; FERNÁNDEZ , R.J.; LATTANZI, F.; OESTERHELD , M.; OYHANTÇABAL, W.; PARUELO, J.; PRAVIA, V.; PIÑEIRO, G. |
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Afiliación : |
SEBASTIÁN H. VILLARINO, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Facultad de Ciencias Agrarias (FCA), Universidad Nacional de Mar del Plata (UNMdP), Argentina.; PRISCILA PINTO, IFEVA-Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Argentina.; TOMAS DELLA CHIESA, IFEVA-Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Argentina.; ESTEBAN G. JOBBÁGY, Grupo de Estudios Ambientales - IMASL, Universidad Nacional de San Luis/ CONICET, Ejército de los Andes 950 (5700), San Luis, Argentina.; GUILLERMO A. STUDDERT, Facultad de Ciencias Agrarias (FCA), Universidad Nacional de Mar del Plata (UNMdP), Argentina.; BRUNO BAZZONI, IFEVA-Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Argentina.; GEORGINA CONTI, Instituto Multidisciplinario de Biología Vegetal, Consejo Nacional de Investigaciones Científicas y Técnicas/ Universidad Nacional de Córdoba, IMBiV (CONICET-UNC), Córdoba, Argentina.; MARIANA RUFINO, Lancaster Environment Centre, Lancaster University, Bailrigg, Lancaster, UK.; ROBERTO ÁLVAREZ, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Facultad de Agronomía, Universidad de Buenos Aires, Argentina.; ROBERT BODDEY, Embrapa Agrobiologia, Seropédica, Rio de Janeiro, Brazil.; CIMÉLIO BAYER, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Brazil.; PAULO C. DE F. CARVALHO, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul, Brazil.; ROBERTO J. FERNÁNDEZ, IFEVA-Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Argentina.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTÍN OESTERHELD, IFEVA-Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Argentina.; WALTER OYHANTÇABAL, Unidad de Sostenibilidad y Cambio Climático, Ministerio de Ganadería, Agricultura y Pesca, Uruguay; JOSÉ PARUELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARIA VIRGINIA PRAVIA NIN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GERVASIO PIÑEIRO, IFEVA-Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Argentina Departamento de Sistemas Ambientales/ Facultad de Agronomía, Universidad de la República, Uruguay. |
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Título : |
The role of South American grazing lands in mitigating greenhouse gas emissions. A reply to: "Reassessing the role of grazing lands in carbon-balance estimations: Meta-analysis and review", by Viglizzo et al., (2019). (Letter). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Science of the Total Environment, 20 October 2020, Volume 740, Article number 140108. Doi: https://doi-org/10.1016/j.scitotenv.2020.140108 |
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DOI : |
10.1016/j.scitotenv.2020.140108 |
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Idioma : |
Inglés |
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Palabras claves : |
ACUMULACION DE CARBONO; CARBON DIOXIDE; CARBON INVENTORY; CARBONO ORGANICO DEL SUELO; DIÓXIDO DE CARBONO; IPCC; MATERIA ORGANICA DEL SUELO; SOIL ORGANIC CARBON; SOIL ORGANIC MATTER MODELLING. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 01511naa a2200445 a 4500
001 1061284
005 2020-09-23
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.scitotenv.2020.140108$2DOI
100 1 $aVILLARINO, S.H.
245 $aThe role of South American grazing lands in mitigating greenhouse gas emissions. A reply to$b"Reassessing the role of grazing lands in carbon-balance estimations: Meta-analysis and review", by Viglizzo et al., (2019). (Letter).$h[electronic resource]
260 $c2020
653 $aACUMULACION DE CARBONO
653 $aCARBON DIOXIDE
653 $aCARBON INVENTORY
653 $aCARBONO ORGANICO DEL SUELO
653 $aDIÓXIDO DE CARBONO
653 $aIPCC
653 $aMATERIA ORGANICA DEL SUELO
653 $aSOIL ORGANIC CARBON
653 $aSOIL ORGANIC MATTER MODELLING
700 1 $aPINTO, P.
700 1 $aDELLA CHIESA, T.
700 1 $aJOBBÁGY, E.G.
700 1 $aSTUDDERT, G.A.
700 1 $aBAZZONI, B.
700 1 $aCONTI, G.
700 1 $aRUFINO, M.
700 1 $aÁLVAREZ, R.
700 1 $aBODDEY , R.
700 1 $aBAYER, C.
700 1 $aDE F CARVALHO, P.C.
700 1 $aFERNÁNDEZ , R.J.
700 1 $aLATTANZI, F.
700 1 $aOESTERHELD , M.
700 1 $aOYHANTÇABAL, W.
700 1 $aPARUELO, J.
700 1 $aPRAVIA, V.
700 1 $aPIÑEIRO, G.
773 $tScience of the Total Environment, 20 October 2020, Volume 740, Article number 140108. Doi: https://doi-org/10.1016/j.scitotenv.2020.140108
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