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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
23/10/2015 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LADO, J.; RODRIGO, M.J.; LÓPEZ-CLIMENT, M.; GÓMEZ-CADENAS, A.; ZACARÍAS, L. |
Afiliación : |
JOANNA LADO LINDNER, IATA (Instituto de Agroquímica y Tecnología de Alimentos); INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JESÚS RODRIGO, IATA (Instituto de Agroquímica y Tecnología de Alimentos); MARÍA LÓPEZ-CLIMENT, Universidad Jaume I de Castellón (ES); AURELIO GÓMEZ-CADENAS, Universidad Jaume I de Castellón (ES); LORENZO ZACARÍAS, IATA (Instituto de Agroquímica y Tecnología de Alimentos). |
Título : |
Implication of the antioxidant system in chilling injury tolerance in the red peel of grapefruit. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Postharvest Biology and Technology, 2016, 111 , art. no. 10121 , pp. 214 - 223 . |
DOI : |
10.1016/j.postharvbio.2015.09.013 |
Idioma : |
Inglés |
Notas : |
Article history: Received 20 February 2015 / Received in revised form 10 September 2015 / Accepted 10 September 2015. |
Contenido : |
ABSTRACT.
Previous observations have indicated that the red peel areas of grapefruit with high lycopene concentrations were more tolerant to CI than yellow peel areas (Lado et al., 2015a). Because lycopene is a carotene with powerful antioxidant capacity, this study investigated whether the CI tolerance of the lycopene-accumulating rind of grapefruit may be due to an enhancement of the enzymatic and/or nonenzymatic antioxidant systems. Total antioxidant capacity, antioxidant metabolite (GSH and AsA) contents, and antioxidant enzyme (GR [glutathione reductase], ascorbate peroxidase [APX], catalase [CAT] and superoxide dismutase [SOD]) activity and gene expression were measured in the peel of Star
Ruby grapefruit with contrasting CI tolerance during storage at 2 C for up to 58 d. The peel of CI-tolerant fruit exhibited a lower lipid peroxidation level (MDA content). The hydrogen peroxide concentration was similar after 3 weeks of storage, when the differences in chilling damage between sensitive and tolerant fruit were noticeable, suggesting that the increase in H2O2 is a response of flavedo cells to cold stress that is not necessarily related to the development of CI. Moreover, CI tolerance was not associated with
enhancement of either total antioxidant capacity or glutathione and AsA contents, indicating that such antioxidant responses may be cold-mediated and not directly linked to chilling tolerance. Analysis of singlet oxygen scavenging capacity by the SOAC assay revealed considerably higher activity in the lycopene-accumulating peel than in the yellow peel at harvest time and throughout the entire cold storage and shelf-life period. Enzymatic activity and gene expression analyses of GR, APX and SOD did not reveal the involvement of these antioxidant enzymes in the protection against CI. However, high CAT activity was detected in the peel of CI-tolerant fruit, although this difference did not correlate with changes in the expression levels of the CAT1 and CAT2 genes. Therefore, the boost in singlet oxygen scavenging capacity is likely the primary factor responsible for CI tolerance in the lycopene-accumulating peel of grapefruit.
@ 2015 Elsevier B.V. All rights reserved. MenosABSTRACT.
Previous observations have indicated that the red peel areas of grapefruit with high lycopene concentrations were more tolerant to CI than yellow peel areas (Lado et al., 2015a). Because lycopene is a carotene with powerful antioxidant capacity, this study investigated whether the CI tolerance of the lycopene-accumulating rind of grapefruit may be due to an enhancement of the enzymatic and/or nonenzymatic antioxidant systems. Total antioxidant capacity, antioxidant metabolite (GSH and AsA) contents, and antioxidant enzyme (GR [glutathione reductase], ascorbate peroxidase [APX], catalase [CAT] and superoxide dismutase [SOD]) activity and gene expression were measured in the peel of Star
Ruby grapefruit with contrasting CI tolerance during storage at 2 C for up to 58 d. The peel of CI-tolerant fruit exhibited a lower lipid peroxidation level (MDA content). The hydrogen peroxide concentration was similar after 3 weeks of storage, when the differences in chilling damage between sensitive and tolerant fruit were noticeable, suggesting that the increase in H2O2 is a response of flavedo cells to cold stress that is not necessarily related to the development of CI. Moreover, CI tolerance was not associated with
enhancement of either total antioxidant capacity or glutathione and AsA contents, indicating that such antioxidant responses may be cold-mediated and not directly linked to chilling tolerance. Analysis of singlet oxygen scavenging capacity by the SOAC assay reveale... Presentar Todo |
Thesagro : |
ANTIOXIDANTES; CITRUS; DANOS POR HELADA; LYCOPENE. |
Asunto categoría : |
-- |
Marc : |
LEADER 03039naa a2200241 a 4500 001 1053719 005 2019-10-15 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.postharvbio.2015.09.013$2DOI 100 1 $aLADO, J. 245 $aImplication of the antioxidant system in chilling injury tolerance in the red peel of grapefruit. 260 $c2015 500 $aArticle history: Received 20 February 2015 / Received in revised form 10 September 2015 / Accepted 10 September 2015. 520 $aABSTRACT. Previous observations have indicated that the red peel areas of grapefruit with high lycopene concentrations were more tolerant to CI than yellow peel areas (Lado et al., 2015a). Because lycopene is a carotene with powerful antioxidant capacity, this study investigated whether the CI tolerance of the lycopene-accumulating rind of grapefruit may be due to an enhancement of the enzymatic and/or nonenzymatic antioxidant systems. Total antioxidant capacity, antioxidant metabolite (GSH and AsA) contents, and antioxidant enzyme (GR [glutathione reductase], ascorbate peroxidase [APX], catalase [CAT] and superoxide dismutase [SOD]) activity and gene expression were measured in the peel of Star Ruby grapefruit with contrasting CI tolerance during storage at 2 C for up to 58 d. The peel of CI-tolerant fruit exhibited a lower lipid peroxidation level (MDA content). The hydrogen peroxide concentration was similar after 3 weeks of storage, when the differences in chilling damage between sensitive and tolerant fruit were noticeable, suggesting that the increase in H2O2 is a response of flavedo cells to cold stress that is not necessarily related to the development of CI. Moreover, CI tolerance was not associated with enhancement of either total antioxidant capacity or glutathione and AsA contents, indicating that such antioxidant responses may be cold-mediated and not directly linked to chilling tolerance. Analysis of singlet oxygen scavenging capacity by the SOAC assay revealed considerably higher activity in the lycopene-accumulating peel than in the yellow peel at harvest time and throughout the entire cold storage and shelf-life period. Enzymatic activity and gene expression analyses of GR, APX and SOD did not reveal the involvement of these antioxidant enzymes in the protection against CI. However, high CAT activity was detected in the peel of CI-tolerant fruit, although this difference did not correlate with changes in the expression levels of the CAT1 and CAT2 genes. Therefore, the boost in singlet oxygen scavenging capacity is likely the primary factor responsible for CI tolerance in the lycopene-accumulating peel of grapefruit. @ 2015 Elsevier B.V. All rights reserved. 650 $aANTIOXIDANTES 650 $aCITRUS 650 $aDANOS POR HELADA 650 $aLYCOPENE 700 1 $aRODRIGO, M.J. 700 1 $aLÓPEZ-CLIMENT, M. 700 1 $aGÓMEZ-CADENAS, A. 700 1 $aZACARÍAS, L. 773 $tPostharvest Biology and Technology, 2016, 111 , art. no. 10121 , pp. 214 - 223 .
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
02/03/2020 |
Actualizado : |
02/03/2020 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
GAUNA, D.; OVIEDO, S.; VIAL, A.; PENA JUNIOR, M. A. G.; CAMPOS, S. K.; SZOSTAKE, J. |
Afiliación : |
DIEGO GAUNA, Instituto Nacional de Tecnologia Agropecuaria, Argentina; SEBASTIAN OVIEDO ALCOBA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRA VIAL, Instituto Nacional de Investigaciones Agropecuarias, Chile; MARCOS ANTONIO GOMES PENA JÚNIOR, dEmpresa Brasileira de Pesquisa Agropecuária, Brazil; SILVIA KANA-DANI CAMPOS, dEmpresa Brasileira de Pesquisa Agropecuária, Brazil; JAVIER SZOSTAK, Instituto Paraguayo de Tecnología Agraria, Paraguay. |
Título : |
Building foresight capabilities in agricultural scientific institutions of the Southern Cone: lessons from a learning-by doing-experience. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
In: FUTURES CONFERENCE, 20., 2019, Finland. Constructing social futures: sustainability, responsibility and power. Finland: University of Turku, 2019. p. 78-79. |
Idioma : |
Portugués |
Contenido : |
The emergence of new technologies, the conservation of biodiversity and genetic resources, the environmental consequences of current agriculture practices, the impacts of climate change, the gaps in 79 adoption of key technologies and the expansion of plant pest and diseases are all examples of current challenges of the food and agriculture system that requires a scientific, policy and future-oriented, regional approach. The Cooperative Program for the Development of Agricultural Technology in the Southern Cone (PROCISUR), integrated by the National Agricultural Research Institutions (NARIs) of the Southern Cone, conducted a foresight exercise to build capabilities on this discipline and to raise awareness about the usefulness of foresight approaches to policy makers. |
Palabras claves : |
Desenvolvimento Sustentável; Produção Agrícola; Projeto de Pesquisa; Sistema de Alimentação. |
Thesagro : |
POLITICAS PUBLICAS. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14229/1/Building-foresight-capabilities-in-agricultural-scientific-institutions-of-the.pdf
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Marc : |
LEADER 01647nam a2200229 a 4500 001 1060845 005 2020-03-02 008 2019 bl uuuu u01u1 u #d 100 1 $aGAUNA, D. 245 $aBuilding foresight capabilities in agricultural scientific institutions of the Southern Cone$blessons from a learning-by doing-experience.$h[electronic resource] 260 $aIn: FUTURES CONFERENCE, 20., 2019, Finland. Constructing social futures: sustainability, responsibility and power. Finland: University of Turku, 2019. p. 78-79.$c2019 520 $aThe emergence of new technologies, the conservation of biodiversity and genetic resources, the environmental consequences of current agriculture practices, the impacts of climate change, the gaps in 79 adoption of key technologies and the expansion of plant pest and diseases are all examples of current challenges of the food and agriculture system that requires a scientific, policy and future-oriented, regional approach. The Cooperative Program for the Development of Agricultural Technology in the Southern Cone (PROCISUR), integrated by the National Agricultural Research Institutions (NARIs) of the Southern Cone, conducted a foresight exercise to build capabilities on this discipline and to raise awareness about the usefulness of foresight approaches to policy makers. 650 $aPOLITICAS PUBLICAS 653 $aDesenvolvimento Sustentável 653 $aProdução Agrícola 653 $aProjeto de Pesquisa 653 $aSistema de Alimentação 700 1 $aOVIEDO, S. 700 1 $aVIAL, A. 700 1 $aPENA JUNIOR, M. A. G. 700 1 $aCAMPOS, S. K. 700 1 $aSZOSTAKE, J.
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