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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
14/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OTERO, A.; GOÑI, C.; JIFON, J.L.; SYVERTSEN, J.P. |
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Afiliación : |
ALVARO RICARDO OTERO CAMA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; CARMEN TERESITA GOÑI ALTUNA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
High temperature effects on citrus orange leaf gas exchange, flowering, fruit quality and yield. (Conference paper). |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Acta Horticulturae, 2011, no.903, p.1069-1074. |
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Serie : |
(Acta Horticulturae; 903). |
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ISBN : |
978-90-66052-97-0 |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2011.903.149 |
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Idioma : |
Inglés |
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Notas : |
ISHS Acta Horticulturae 903: IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. Editor: T.L. Robinson.
Publication date: 1 August 2011 |
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Contenido : |
ABSTRACT.
High midday temperatures in subtropical citrus growing regions can lead to excessively high leaf temperature (Tlf) and large leaf-to-air vapor pressure difference (D) in sun exposed leaves. This heat stress reduced net CO 2 assimilation (AC), growth, fruit yield and quality. 50% shading reduced average mid-day Tlf and increased AC, stomatal conductance (gs) and leaf water use efficiency, but decreased the internal concentration of CO 2 (Ci) in the mesophyll compared with sun-exposed trees throughout the season. However, shade had little effect on leaf transpiration. Heat stress increased non-stomatal limitations to AC in the mesophyll of sunlit leaves that were greater than stomatal limitations. Shade treatment reduced leaf carbohydrates but did not affect tree canopy volume or fruit size. Shading in the late fall until harvest increased orange yield and juice content more than compensated for the lower total soluble sugars (TSS) and resulted in net increases in TSS per tree. Shaded 'Navel' orange fruit developed better external color but lower TSS than sunexposed fruit. Shade did not affect orange fruit yields. In warm citrus producing regions, shading could improve photosynthesis and fruit quality especially in young trees where most of the leaves are exposed to direct sunlight. Improved fruit color, yield and/or TSS per hectare of high-value varieties could offset the costs of particle film sprays or shading in commercial orchards. |
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Thesagro : |
CARBONO; CITRUS; COLOR; FOTOSÍNTESIS; MARCADO DEL COLOR. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02460naa a2200277 a 4500
001 1051053
005 2019-10-15
008 2011 bl uuuu u00u1 u #d
020 $a978-90-66052-97-0
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2011.903.149$2DOI
100 1 $aOTERO, A.
245 $aHigh temperature effects on citrus orange leaf gas exchange, flowering, fruit quality and yield. (Conference paper).$h[electronic resource]
260 $c2011
490 $a(Acta Horticulturae; 903).
500 $aISHS Acta Horticulturae 903: IX International Symposium on Integrating Canopy, Rootstock and Environmental Physiology in Orchard Systems. Editor: T.L. Robinson. Publication date: 1 August 2011
520 $aABSTRACT. High midday temperatures in subtropical citrus growing regions can lead to excessively high leaf temperature (Tlf) and large leaf-to-air vapor pressure difference (D) in sun exposed leaves. This heat stress reduced net CO 2 assimilation (AC), growth, fruit yield and quality. 50% shading reduced average mid-day Tlf and increased AC, stomatal conductance (gs) and leaf water use efficiency, but decreased the internal concentration of CO 2 (Ci) in the mesophyll compared with sun-exposed trees throughout the season. However, shade had little effect on leaf transpiration. Heat stress increased non-stomatal limitations to AC in the mesophyll of sunlit leaves that were greater than stomatal limitations. Shade treatment reduced leaf carbohydrates but did not affect tree canopy volume or fruit size. Shading in the late fall until harvest increased orange yield and juice content more than compensated for the lower total soluble sugars (TSS) and resulted in net increases in TSS per tree. Shaded 'Navel' orange fruit developed better external color but lower TSS than sunexposed fruit. Shade did not affect orange fruit yields. In warm citrus producing regions, shading could improve photosynthesis and fruit quality especially in young trees where most of the leaves are exposed to direct sunlight. Improved fruit color, yield and/or TSS per hectare of high-value varieties could offset the costs of particle film sprays or shading in commercial orchards.
650 $aCARBONO
650 $aCITRUS
650 $aCOLOR
650 $aFOTOSÍNTESIS
650 $aMARCADO DEL COLOR
700 1 $aGOÑI, C.
700 1 $aJIFON, J.L.
700 1 $aSYVERTSEN, J.P.
773 $tActa Horticulturae, 2011, no.903, p.1069-1074.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
03/12/2020 |
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Actualizado : |
03/12/2020 |
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Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
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Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
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Título : |
Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
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DOI : |
10.1007/s11240-020-01891-6 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
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Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
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Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 03007naa a2200301 a 4500
001 1061539
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11240-020-01891-6$2DOI
100 1 $aNARANCIO, R.
245 $aCorrection to$bApplication of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6).$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020.
520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V.
653 $aAgrobacterium-mediated transformation
653 $aDelayed leaf senescence
653 $aMultigene
653 $aT-DNA
700 1 $aDING, Y.-L.
700 1 $aLIN, Y.-H.
700 1 $aSAHAB, S.
700 1 $aPANTER, S.
700 1 $aHAYES, M.
700 1 $aJOHN, U.
700 1 $aANDERSON, H.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
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