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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
07/10/2014 |
Actualizado : |
05/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ALARCÓN-REVERTE, R.; GARCIA, M.A.; WATSON, S.B.; ABDALLAH, I.; SABATÉ, S.; HERNÁNDEZ, M.J.; DAYAN ,F.E.; FISCHER, A.J. |
Afiliación : |
MILTON ALEJANDRO GARCIA LATASA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Pest Management Science, v. 71, n. 7, p. 996-2007, 2015. |
ISSN : |
1526-4998. |
DOI : |
10.1002/ps.3878 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 |
Contenido : |
Abstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different populations may carry different resistance mechanisms and different mechanisms can
act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant
expression of EPSPS sensitivity to glyphosate. MenosAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different popula... Presentar Todo |
Palabras claves : |
5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; EPSPS EXPRESSION; EPSPS INHIBITION ASSAY; POLYPLOIDY; RESISTANCE MECHANISM. |
Thesagro : |
RESISTENCIA A GLIFOSATO. |
Asunto categoría : |
H60 Malezas y escardas |
Marc : |
LEADER 02883naa a2200313 a 4500 001 1050961 005 2019-11-05 008 2015 bl uuuu u00u1 u #d 022 $a1526-4998. 024 7 $a10.1002/ps.3878$2DOI 100 1 $aALARCÓN-REVERTE, R. 245 $aConcerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California.$h[electronic resource] 260 $c2015 500 $aArticle history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 520 $aAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide, was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance. RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption, translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants. Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants. CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same population, individuals from different populations may carry different resistance mechanisms and different mechanisms can act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant expression of EPSPS sensitivity to glyphosate. 650 $aRESISTENCIA A GLIFOSATO 653 $a5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE 653 $aEPSPS EXPRESSION 653 $aEPSPS INHIBITION ASSAY 653 $aPOLYPLOIDY 653 $aRESISTANCE MECHANISM 700 1 $aGARCIA, M.A. 700 1 $aWATSON, S.B. 700 1 $aABDALLAH, I. 700 1 $aSABATÉ, S. 700 1 $aHERNÁNDEZ, M.J. 700 1 $aDAYAN ,F.E. 700 1 $aFISCHER, A.J. 773 $tPest Management Science$gv. 71, n. 7, p. 996-2007, 2015.
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
04/05/2018 |
Actualizado : |
04/05/2018 |
Tipo de producción científica : |
Presentaciones Orales |
Autor : |
CLARIGET, J.M.; OTAÑO, C.; PEREZ, E.; ZARZA, R.; LEMA, O.M.; QUINCKE, A.; GARCIA, A.; LATTANZI, F. |
Afiliación : |
JUAN MANUEL CLARIGET BRIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS ALBERTO OTAÑO LUNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDUARDO FABIAN PEREZ ARRUTTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODRIGO TABARE ZARZA FUENTES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OSCAR MARIO LEMA QUEIJO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN ANDRES QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MILTON ALEJANDRO GARCIA LATASA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Efecto acumulado del pastoreo de cultivos de cobertura: 1. Desempeño animal y compactación del suelo.
2. Cobertura de suelo, enmalezamiento y rendimiento de soja. [Presentación oral]. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
En: CONGRESO ASOCIACIÓN URUGUAYA DE PRODUCCIÓN ANIMAL (6º, Marzo, 2018, Tacuarembó, Uruguay). Tacuarembó: AUPA, 2018. |
Idioma : |
Español |
Contenido : |
Objetivo: Evaluar el efecto del cultivo de cobertura (Avena) sin y con pastoreo a dos cargas contrastantes (3 y 6 Terneros/ha), sobre la producción animal, las propiedades físicas del suelo y el rendimiento de soja posterior.
Conclusiones:
El pastoreo de cultivos de cobertura retirando los animales cuando el suelo alcanza el límite plástico, permitió lograr adecuadas producciones de carne/ha sin producir compactación en el mismo.
El pastoreo de cultivos de cobertura retirando los animales cuando el suelo alcanza el límite plástico no afectó el rendimiento de sojas posteriores.
Es necesario evaluar la carga animal óptima (o remanente mínimo) para no afectar negativamente el nivel de cobertura del suelo y de enmalezamiento. |
Thesagro : |
PASTOREO. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9482/1/AUPA-2018-Juan-Clariget.ppt
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Marc : |
LEADER 01494nam a2200205 a 4500 001 1058546 005 2018-05-04 008 2018 bl uuuu u00u1 u #d 100 1 $aCLARIGET, J.M. 245 $aEfecto acumulado del pastoreo de cultivos de cobertura$b1. Desempeño animal y compactación del suelo. 2. Cobertura de suelo, enmalezamiento y rendimiento de soja. [Presentación oral].$h[electronic resource] 260 $aEn: CONGRESO ASOCIACIÓN URUGUAYA DE PRODUCCIÓN ANIMAL (6º, Marzo, 2018, Tacuarembó, Uruguay). Tacuarembó: AUPA$c2018 520 $aObjetivo: Evaluar el efecto del cultivo de cobertura (Avena) sin y con pastoreo a dos cargas contrastantes (3 y 6 Terneros/ha), sobre la producción animal, las propiedades físicas del suelo y el rendimiento de soja posterior. Conclusiones: El pastoreo de cultivos de cobertura retirando los animales cuando el suelo alcanza el límite plástico, permitió lograr adecuadas producciones de carne/ha sin producir compactación en el mismo. El pastoreo de cultivos de cobertura retirando los animales cuando el suelo alcanza el límite plástico no afectó el rendimiento de sojas posteriores. Es necesario evaluar la carga animal óptima (o remanente mínimo) para no afectar negativamente el nivel de cobertura del suelo y de enmalezamiento. 650 $aPASTOREO 700 1 $aOTAÑO, C. 700 1 $aPEREZ, E. 700 1 $aZARZA, R. 700 1 $aLEMA, O.M. 700 1 $aQUINCKE, A. 700 1 $aGARCIA, A. 700 1 $aLATTANZI, F.
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