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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
17/08/2023 |
Actualizado : |
17/08/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. |
Afiliación : |
MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
Título : |
Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. |
ISSN : |
2076-2607 (electronic). |
DOI : |
10.3390/microorganisms11071704 |
Idioma : |
Inglés |
Notas : |
Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). |
Contenido : |
ABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalen... Presentar Todo |
Palabras claves : |
Dairy calf; MDR STEC; Mortality; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Shiga toxin-producing E. coli. |
Asunto categoría : |
L01 Ganadería |
URL : |
https://www.mdpi.com/2076-2607/11/7/1704/pdf
|
Marc : |
LEADER 04099naa a2200289 a 4500 001 1064289 005 2023-08-17 008 2023 bl uuuu u00u1 u #d 022 $a2076-2607 (electronic). 024 7 $a10.3390/microorganisms11071704$2DOI 100 1 $aFERNÁNDEZ, M. 245 $aShiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford, Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). 520 $aABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. 653 $aDairy calf 653 $aMDR STEC 653 $aMortality 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aShiga toxin-producing E. coli 700 1 $aCASAUX, M.L. 700 1 $aFRAGA, M. 700 1 $aVIGNOLI, R. 700 1 $aBADO, I. 700 1 $aZUNINO, P. 700 1 $aUMPIÉRREZ, A. 773 $tMicroorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS.
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Registros recuperados : 44 | |
21. | | LOZANO, J.; FERNÁNDEZ-CIGANDA, S.; FABIUS, S.; PICCINI, C.; SCORZA, C.; ZUNINO, P. Efecto tipo-antidepresivo inducido por el tratamiento crónico de una cepa del grupo lactobacillus plantarum en ratas y asociación con la microbiota intestinal. 395. (resúmen) Conferencia 21. Mesa Área Biotecnología. Organiza SUM (Sociedad Uruguaya de Microbiología). In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.86. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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23. | | FRAGA, M.; PERELMUTER, K.; VALENCIA, M.J.; MARTÍNEZ, M.; ABIN-CARRIQUIRY, A.; CAJARVILLE, C.; ZUNINO, P. Evaluation of native potential probiotic bacteria using an in vitro ruminal fermentation system. Annals of Microbiology, September 2014, Volume 64, Issue 3, pp 1149-1156. Article history:Received: 11 July 2013 /Accepted: 29 October 2013.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 1 |
Biblioteca(s): INIA La Estanzuela. |
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24. | | RABAZA, A.; BANCHERO, G.; CAJARVILLE,C.; ZUNINO, P.; BRITO, A.; REPETTO, J.; FRAGA, M. Effects of feed withdrawal duration on animal behaviour, rumen microbiota and blood chemistry in feedlot cattle: implications for rumen acidosis. Animal, Volume 14 , Issue 1 , January 2020 , pp. 66 - 77. DOI: https://doi.org/10.1017/S1751731119001538 Article history: Received 22 October 2018 // Accepted 13 June 2019.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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25. | | CASTELLI, L.; BALBUENA, S.; BRANCHICCELA, B.; ZUNINO, P.; LIBERTI, J.; ENGEL, P.; ANTÚNEZ, K. Impact of chronic exposure to sublethal doses of glyphosate on honey bee immunity, gut microbiota and infection by pathogens. Microorganisms, April 2021, Volume 9; Issue 4, Article number 845. OPEN ACCESS. https://doi.org/10.3390/microorganisms9040845 Article history: Received: 16 March 2021/Accepted: 31 March 2021/Published: 15 April 2021.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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26. | | PÉREZ-RUCHEL, A.; REPETTO, J.L.; FRAGA, M.; PERELMUTER, K.; ZUNINO, P.; CAJARVILLE, C. La restricción en el tiempo de acceso al forraje en ovinos alimentados con pastura de buena calidad afecta algunos grupos microbianos ruminales.(The restriction in the time of access to forage in ovine fed a high quality pasture affect groups of ruminal microorganisms). Veterinaria (Montevideo), v. 50, n. 194, p. 22-33, 2014.Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : B - 5 |
Biblioteca(s): INIA La Estanzuela. |
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27. | | FERNÁNDEZ, S.; FRAGA, M.; SILVERYRA, E.; RABAZA, A.; PLA, M.; ZUNINO, P. Probióticos nativos para terneros bajo sistema de cría intensiva y semi-intensiva: selección de cepas y ensayos de persitencia en el tracto gastrointestinal. In: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016. p.193-195.Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA La Estanzuela. |
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28. | | LOZANO, J.; FERNÁNDEZ-CIGANDA, S.; GONZÁLEZ REVELLO, A.; HIRIGOYEN, D.; MARTÍNEZ, M.; SCORZA, C.; ZUNINO, P. Probiotic potential of GABA-producing lactobacilli isolated from Uruguayan artisanal cheese starter cultures. Journal of Microbiology, 2022, volume 133, issue 3, pages 1610-1619. doi: https://doi.org/10.1111/jam.15664 Article history: Received 18 March 2022; Revised 5 May 2022; Accepted 6 June 2022; Published online September 2022. -- Corresponding author: Zunino, P.; Department of Microbiology, Instituto de Investigaciones Biológicas Clemente...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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29. | | DE LOS SANTOS, R.; ZUNINO, P.; GONZÁLEZ REVELLO, A.; GIL, A.; CAL, K.; HIRIGOYEN, D. Producción de biofilms de Stahylococcus aureus aislados de leche en tres medios de cultivo. Carnes & Alimentos: La revista de carnes y alimentos del Uruguay, v. 17, n.57, p.18-22,2016.Tipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA La Estanzuela. |
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30. | | INVERNIZZI, C.; ANTÚNEZ, K.; CAMPA, J.P.; HARRIET, J.; MENDOZA, Y.; SANTOS, E.; ZUNINO, P. Situación sanitaria de las abejas melíferas en Uruguay. Veterinaria (Montevideo), v. 47, n. 181, p. 15-27, 2011. Falta CIALISTipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA La Estanzuela. |
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31. | | FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A....Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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32. | | FRAGA, M.; FERNÁNDEZ, S.; PERELMUTER, K.; POMMIÉS, N.; CAJARVILLE, C.; ZUNINO, P. The use of Prevotella bryantii 3C5 for modulation of the ruminal environment in an ovine model. Veterinary Microbiology. Brazilian Journal of Microbiology, November 2018, volume 49, Supplement 1, Pages 101-106. OPEN ACCESS. Article history: Received 24 April 2018 // Accepted 17 July 2018 // Available online 25 August 2018.
Open Access funded by Sociedade Brasileira de Microbiologia. Under a Creative Commons license.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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34. | | BRANCHICCELA, B.; CASTELLI, L.; DIAZ, S.; INVERNIZZI, C; MENDOZA, Y.; SANTOS, E.; SILVA, C.; ZUNINO, P.; ANTUNEZ, K, Can pollen supplementation mitigate the impact of nutritional stress on honey bee colonies? Journal of Apicultural Research, 2023, Volume 62, Issue 2, pp. 294-302. Doi: https://doi.org/10.1080/00218839.2021.1888537 Article history:Received 27 May 2020/ Accepted 08 Dec 2020/ Published online: 11 Mar 2021.Corresponding author. Email: bbranchiccela@inia.org.uy. This work was supported by the University of the Republic,Uruguay; under grant CSIC C624 and...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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35. | | Carro, S.; Fraga, M.; Perelmuter, K.; Vera, S.; Zunino, P.; Acosta, Y.; Barneche, M.; Villagrán, M.; Calliari, A.; Delucchi, I. Bacterias ácido lácticas en quesos artesanales: importancia de la producción de bacteriocinas Carnes y alimentos, 2009, v. 10, no. 30, p. 31-35Biblioteca(s): INIA Tacuarembó. |
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36. | | ANTÚNEZ, K.; ARREDONDO, D.; CASTELLI, L.; SILVA, F.; AÑON, G.; HARRIET, J.; CAMPÁ, J.; INVERNIZZI, C.; ZUNINO, P. Desarrollo de una estrategia natural para el control de Varroa destructor, integrando el uso de probióticos y productos orgánicos. Montevideo (UY): INIA, 2019. 31 p. (Serie FPTA-INIA; 75). Proyecto FPTA-329: Desarrollo de una estrategia natural para el control de Varroa destructor, integrando el uso de probióticos y productos orgánicos. Periodo de ejecución: Febrero 2014 ? Junio 2017. Institución Ejecutora: MEC/ IIBCE...Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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37. | | ANTÚNEZ, K.; ANIDO, M.; BRANCHICCELA, M.B.; HARRIET, J.; CAMPÁ, J.; INVERNIZZI, C.; MARTIN-HERNÁNDEZ, R.; HIGES, M.; ZUNINO, P. Despoblación de colmenas Montevideo (UY): INIA, 2013. 36 p. (Serie FPTA-INIA; 41) Proyecto FPTA 258: Despoblación de colmenas: determinación de sus causas en Uruguay. Período de Ejecución: Febrero 2009 - Octubre 2011Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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38. | | FABIUS, S.; URBANAVICIUS, J.; FERNÁNDEZ-CIGANDA, S.; PRIETO, J.P.; LOZANO, J.; PICCINI, C.; ZUNINO, P.; SCORZA, C. Efecto del pretratamiento oral con Lactobacillus SPP. en la sensibilización locomotora inducida por cocaína y su influencia en la microbiota intestinal. [resumen] Conferencia 3. Mesa Sociedad de Neurociencias del Uruguay. Mesa VI. Presentaciones orales seleccionadas de estudiantes. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.38. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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39. | | UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uyTipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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40. | | PÉREZ-RUCHEL, A.; BRITOS, A.; ALVARADO, A.; FERNÁNDEZ-CIGANDA, S.; GADEYNE, F.; BUSTOS, M.; ZUNINO, P.; CAJARVILLE, C. Impact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system. Animal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763 Article history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 44 | |
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