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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
04/09/2019 |
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Actualizado : |
16/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ROVIRA, P.J.; MCALLISTER, T.; LAKIN, S.M.; COOK, S.R.; DOSTER, E.; NOYES, N. R.; WEINROTH, M.D.; YANG, X.; PARKER, J. K.; BOUCHER, C.; BOOKER, C. W.; WOENER, D. R.; BELK, K. E.; MORLEY, P. S. |
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Afiliación : |
PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; TIM MCALLISTER, Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada.; STEVEN M. LAKIN, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.; SHAUN R. COOK, Alberta Agricultural and forestry, Lethbridge, Canada.; ENRIQUE DOSTER, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, USA.; NOELLE R. NOYES, Veterinary Population Medicine Department, University of Minnesota, USA.; MAGGIE D. WEINROTH, Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; XIANG YANG, Department of Animal Science, University of California, Davis, USA.; JENNIFER K. PARKER, Deparment of Molecular Biosciences, University of Florida, Gainesville, FL, USA.; CHRISTINA BOUCHER, Deparment of Computer and Information Science and Engineering, University of Florida, Gainessville, FL, USA.; CALVIN W. BOOKER, Feedlot Health Management Services, Okotoks, AB, Canada.; DALE R. WOEMER, Deparment of Animal and Food Sciences, College of Agricultural Sciences & Natural Resources, Texas University, TX, USA.; KEITH E. BELK, Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, USA.; PAUL S. MORLEY, VERO, Veterinary Education, Research , and Outreach Program, Texas A&M University and West Texas A&M University, Canyon, TX, USA. |
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Título : |
Characterization of the microbial resistome in conventional and "raised without antibiotics" beef and dairy production systems. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Frontier in Microbiology, September 2019. v. 10, article 1980, 11 p. OPEN ACCESS. |
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DOI : |
10.3389/fmicb.2019.01980 |
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Idioma : |
Inglés |
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Notas : |
Received 18 March 2019 // Accepted 12 August 2019 // Published 4 September 2019. |
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Contenido : |
Metagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle source, management practices, diet, horizontal ARGs transfer, and co-selection of resistance), in addition to antimicrobial use, could have impacted resistome profiles. For that reason, we could not establish a cause-effect relationship between antimicrobial use and AMR, although ARGs in feces and effluents were associated with drug classes used to treat animals according to farms' records (tetracyclines and macrolides in feedlots, b-lactams in dairies), whereas ARGs in soil were dominated by multidrug resistance.
Characterization of the "resistance potential" of animal-derived and environmental samples is the first step toward incorporating metagenomic approaches into AMR surveillance in agricultural systems. Further research is needed to assess the publichealth risk associated with different microbial resistomes. MenosMetagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle sour... Presentar Todo |
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Palabras claves : |
ANTIBIOTIC RESISTANCE; CATTLE; CATTLE BEEF; DAIRY CATTLE; METAGENOMICA; METAGENOMICS; MICROBIOMA; MICROBIOME; RESISTENCIA A ANTIBIÓTICOS; RESISTOME. |
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Thesagro : |
BOVINOS; BOVINOS DE CARNE; GANADO LECHERO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13237/1/Rovira-Front-microb-2019.pdf
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Marc : |
LEADER 03681naa a2200457 a 4500
001 1060137
005 2021-03-16
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2019.01980$2DOI
100 1 $aROVIRA, P.J.
245 $aCharacterization of the microbial resistome in conventional and "raised without antibiotics" beef and dairy production systems.$h[electronic resource]
260 $c2019
500 $aReceived 18 March 2019 // Accepted 12 August 2019 // Published 4 September 2019.
520 $aMetagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR). We characterized the microbial resistome in livestock operations raising cattle conventionally (CONV) or without antibiotic exposures (RWA) using shotgun metagenomics. Samples of feces, wastewater from catchment basins, and soil where wastewater was applied were collected from CONV and RWA feedlot and dairy farms. After DNA extraction and sequencing, shotgun metagenomic reads were aligned to reference databases for identification of bacteria (Kraken) and antibiotic resistance genes (ARGs) accessions (MEGARes). Differences in microbial resistomes were found across farms with different production practices (CONV vs. RWA), types of cattle (beef vs. dairy), and types of sample (feces vs. wastewater vs. soil). Feces had the greatest number of ARGs per sample (mean = 118 and 79 in CONV and RWA, respectively), with tetracycline efflux pumps, macrolide phosphotransferases, and aminoglycoside nucleotidyltransferases mechanisms of resistance more abundant in CONV than in RWA feces. Tetracycline and macrolide-lincosamide-streptogramin classes of resistance were more abundant in feedlot cattle than in dairy cow feces, whereas the b-lactam class was more abundant in dairy cow feces. Lack of congruence between ARGs and microbial communities (procrustes analysis) suggested that other factors (e.g., location of farms, cattle source, management practices, diet, horizontal ARGs transfer, and co-selection of resistance), in addition to antimicrobial use, could have impacted resistome profiles. For that reason, we could not establish a cause-effect relationship between antimicrobial use and AMR, although ARGs in feces and effluents were associated with drug classes used to treat animals according to farms' records (tetracyclines and macrolides in feedlots, b-lactams in dairies), whereas ARGs in soil were dominated by multidrug resistance. Characterization of the "resistance potential" of animal-derived and environmental samples is the first step toward incorporating metagenomic approaches into AMR surveillance in agricultural systems. Further research is needed to assess the publichealth risk associated with different microbial resistomes.
650 $aBOVINOS
650 $aBOVINOS DE CARNE
650 $aGANADO LECHERO
653 $aANTIBIOTIC RESISTANCE
653 $aCATTLE
653 $aCATTLE BEEF
653 $aDAIRY CATTLE
653 $aMETAGENOMICA
653 $aMETAGENOMICS
653 $aMICROBIOMA
653 $aMICROBIOME
653 $aRESISTENCIA A ANTIBIÓTICOS
653 $aRESISTOME
700 1 $aMCALLISTER, T.
700 1 $aLAKIN, S.M.
700 1 $aCOOK, S.R.
700 1 $aDOSTER, E.
700 1 $aNOYES, N. R.
700 1 $aWEINROTH, M.D.
700 1 $aYANG, X.
700 1 $aPARKER, J. K.
700 1 $aBOUCHER, C.
700 1 $aBOOKER, C. W.
700 1 $aWOENER, D. R.
700 1 $aBELK, K. E.
700 1 $aMORLEY, P. S.
773 $tFrontier in Microbiology, September 2019.$gv. 10, article 1980, 11 p. OPEN ACCESS.
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INIA Treinta y Tres (TT) |
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| Registros recuperados : 13 | |
| 1. |  | LOMBARDO, P.; DELLACASSA, E.; PEREZ, E. Bioactividad de aceites esenciales contra patógenos de los cítricos. In: JORNADA NACIONAL DE FITOPATOLOGÍA, 3; JORNADA NACIONAL DE PROTECCIÓN VEGETAL, 1., 3 SETIEMBRE 2015, MONTEVIDEO, URUGUAY. Libro de Resúmenes. Montevideo (Uruguay) : SUFIT, 2015. p. 19 Financiamiento: Instituto Nacional de Investigación Agropecuaria (INIA), Facultad de Agronomía, UdelaR y Comisión Sectorial de Investigación Científica (CSIC).| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | LOMBARDO, P.; LEONI, C.; ALANIZ, S.; MONDINO, P. Cercospora leaf spot of olive in Uruguay. Phytopathologia Mediterranea. 2023, Volume 62, Issue 3, pp. 413-426. https://doi.org/10.36253/phyto-14675 -- OPEN ACCESS Article history: Accepted 7 November 2023, PublishedPublished 30 December 2023. -- Correspondence: Lombardo, P.; Departamento de Ciencias Biológicas, CENUR Litoral Norte, Universidad de la República, Gral. Rivera 1350 CP 50000,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | BISIO, L.; VIGNALE, B.; LOMBARDO, P.; CARRAU, F. Evaluación de nuevos portainjertos híbridos de trifolia en combinación con la mandarina Ellendale en Salto. In: Congreso Nacional de Horticultura, 8.; Seminario Regional de Frutilla, 2001, Salto, Uruguay. Resúmenes. Montevideo (Uruguay): S635 CONr 8UH; INIA, 2001. p. 56 "Sociedad Uruguaya de Horticultura; Instituto Nacional de Investigación Agropecuaria, Uruguay"| Biblioteca(s): INIA Las Brujas; INIA Salto Grande; INIA Tacuarembó; INIA Treinta y Tres. |
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| 8. | | CABRERA, D.; VIGNALE, B.; NEBEL, J.P.; LOMBARDO, P.; RODRIGUEZ, P.; ZOPPOLO, R.; PEREIRA, C. Avances en la selección de guayabo del país. In: INIA, Programa Nacional Investigación Producción Frutícola; Universidad de la República (UdelaR), Facultad de Agronomía; MGAP, DGF (Dirección General Forestal). Encuentro Nacional de Frutos Nativos, 6. Canelones (Uruguay): INIA, 2012. p.48-53 (INIA Serie Actividades de Difusión; 679)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 9. | | VIGNALE, B.; CABRERA, D.; NEBEL, J.P.; LOMBARDO, P.; RODRIGUEZ, P.; ZOPPOLO, R.; PEREIRA, C. Selección de frutas nativas. Avances. In: INIA, Programa Nacional Investigación Producción Frutícola; Universidad de la República (UdelaR), Facultad de Agronomía; MGAP, DGF (Dirección General Forestal). Encuentro Nacional de Frutos Nativos, 6. Canelones (Uruguay): INIA, 2012. p.38-44 (INIA Serie Actividades de Difusión; 679)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 10. | | PRITSCH, C.; MAZZELLA, C.; VAIO, M.; QUEZADA, M.; DA CRUZ, I.; LOMBARDO, P.; CABRERA, D.; VIGNALE, B. Hacia la caracterización de la estructura del genoma de la especie frutal nativa Acca Sellowiana (Berg.) Burret: abordajes genéticos, citológicos y moleculares. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola; Facultad de Agronomía, Universidad de la República; Dirección General Forestal, MGAP. Encuentro Nacional de Frutos Nativos, 5. Salto (Uruguay): INIA, 2010. p.10 (INIA Serie Actividades de Difusión ; 602)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 11. | | PEREZ, E.; LOMBARDO, P.; UMPIÉRREZ, N.; MINTEGUIAGA, M.; ALVES, P.; BLANCO, O.; GUIMARAENS, A.; RODRÍGUEZ, F.; PARDO, H.; DELLACASSA, E. Fungicidas botánicos. Aceites esenciales de plantas nativas que controlan enfermedades de poscosecha de los cítricos. Revista INIA Uruguay, 2015, No.40, p.52-56. (Revista INIA; 40)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 12. | | VIGNALE, B.; LOMBARDO, P.; BISIO, L.; VIETTRO, J.A.; LÓPEZ, H.; FAGÚNDEZ V.; CABRERA, D.; ZOPPOLO, R.; NEBEL, J.P. Selección de frutas nativas. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola; Facultad de Agronomía, Universidad de la República; Dirección General Forestal, MGAP. Encuentro Nacional de Frutos Nativos, 5. Salto (Uruguay): INIA, 2010. p.38-40 (INIA Serie Actividades de Difusión ; 602)| Biblioteca(s): INIA Las Brujas. |
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| 13. | | PRITSCH, C.; MAZZELLA, C.; QUEZADA, M.; DA CRUZ, I.; VÁZQUEZ, S.; GAIERO, P.; VAIO, M.; SILVA, P.; BACCINO, E.; LOMBARDO, P.; HINRICHSEN, P.; GARCIA, A.A.F.; CABRERA, D.; VIGNALE, B. Avances en estudios genómicos en guayabo del país (Acca sellowiana). In: INIA, Programa Nacional Investigación Producción Frutícola; Universidad de la República (UdelaR), Facultad de Agronomía; MGAP, DGF (Dirección General Forestal). Encuentro Nacional de Frutos Nativos, 6. Canelones (Uruguay): INIA, 2012. p.16-22 (INIA Serie Actividades de Difusión; 679)| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 13 | |
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