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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
24/10/2023 |
Actualizado : |
24/10/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
DOI : |
10.1111/imb.12875 |
Idioma : |
Inglés |
Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
Asunto categoría : |
L01 Ganadería |
URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
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Marc : |
LEADER 02928naa a2200313 a 4500 001 1064356 005 2023-10-24 008 2023 bl uuuu u00u1 u #d 022 $a0962-1075 (print); 1365-2583 (electronic). 024 7 $a10.1111/imb.12875$2DOI 100 1 $aNOVAS, R. 245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- 520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aPartnership for the goals - Goal 17 653 $aPest control 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aScrewworm 653 $aSustainable Development Goals (SDGs) 653 $aU6 promoter 700 1 $aBASIKA, T. 700 1 $aWILLIAMSON, M. E. 700 1 $aFRESIA, P. 700 1 $aMENCHACA, A. 700 1 $aSCOTT, M. J. 773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
03/10/2019 |
Actualizado : |
03/10/2019 |
Tipo de producción científica : |
Informes Agroclimáticos |
Autor : |
GIMÉNEZ, A.; CAL, A.; TISCORNIA, G.; SCHIAVI, C. |
Afiliación : |
AGUSTIN EDUARDO GIMÉNEZ FUREST, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIAN TABARE CAL ALVAREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS IGNACIO SCHIAVI RAMPELBERG, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Informe agroclimático 2019 - Situación a Setiembre. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 2019. |
Páginas : |
4 p. |
Serie : |
(Informe Agroclimático; 161) |
Idioma : |
Español |
Palabras claves : |
AGROCLIMA; AGROCLIMATOLOGÍA; BOLETIN AGROCLIMÁTICO; CARACTERIZACIÓN AGROCLIMÁTICA; DIRECCION VIENTO; ESTACIONES AGROMETEOROLOGICAS; ESTACIONES AUTOMATICAS; ESTACIONES INIA; ESTADO DEL TIEMPO; ESTRÉS HÍDRICO; GRAFICAS AGROCLIMATICOS; GRAS; HELIOFANOGRAFO; INFORMACION SATELITAL; INFORME AGROCLIMÁTICO 2019; INUNDACIONES; LLUVIAS DIARIAS; MAXIMA; MEDIA; MINIMA; PANEL SOLAR; PERSPECTIVAS CLIMATICAS; PLUVIOMETRO; PRECIPITACION NACIONAL; PREVENCION HELADAS; PRONOSTICO; SENSOR; SIMETRICO; TANQUE A; TERMOCUPLAS; TERMOHIDROGRAFO; VARIABLES AGROCLIMATICAS; VELETA. |
Thesagro : |
AGROCLIMATOLOGIA; CAMBIO CLIMATICO; CLIMA; CLIMATOLOGIA; ESTACIONES METEOROLOGICAS; ESTRES HIDRICO; EVAPORACION; EVAPOTRANSPIRACION; HUMEDAD; HUMEDAD RELATIVA; LLUVIA; METEOROLOGIA; PERSPECTIVAS; PLUVIOMETROS; PRONOSTICO DEL TIEMPO; SENSORES; SISTEMAS; SISTEMAS DE INFORMACION; SUELO; TEMPERATURA; TERMOMETROS. |
Asunto categoría : |
P40 Meteorología y climatología |
URL : |
http://www.inia.uy/Publicaciones/Documentos%20compartidos/Informe%20agroclimatico%20INIA-GRAS%20Setiembre%20de%202019.pdf
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Marc : |
LEADER 02130nam a2200805 a 4500 001 1060272 005 2019-10-03 008 2019 bl uuuu u0uu1 u #d 100 1 $aGIMÉNEZ, A. 245 $aInforme agroclimático 2019 - Situación a Setiembre.$h[electronic resource] 260 $aMontevideo (Uruguay): INIA$c2019 300 $a4 p. 490 $a(Informe Agroclimático; 161) 650 $aAGROCLIMATOLOGIA 650 $aCAMBIO CLIMATICO 650 $aCLIMA 650 $aCLIMATOLOGIA 650 $aESTACIONES METEOROLOGICAS 650 $aESTRES HIDRICO 650 $aEVAPORACION 650 $aEVAPOTRANSPIRACION 650 $aHUMEDAD 650 $aHUMEDAD RELATIVA 650 $aLLUVIA 650 $aMETEOROLOGIA 650 $aPERSPECTIVAS 650 $aPLUVIOMETROS 650 $aPRONOSTICO DEL TIEMPO 650 $aSENSORES 650 $aSISTEMAS 650 $aSISTEMAS DE INFORMACION 650 $aSUELO 650 $aTEMPERATURA 650 $aTERMOMETROS 653 $aAGROCLIMA 653 $aAGROCLIMATOLOGÍA 653 $aBOLETIN AGROCLIMÁTICO 653 $aCARACTERIZACIÓN AGROCLIMÁTICA 653 $aDIRECCION VIENTO 653 $aESTACIONES AGROMETEOROLOGICAS 653 $aESTACIONES AUTOMATICAS 653 $aESTACIONES INIA 653 $aESTADO DEL TIEMPO 653 $aESTRÉS HÍDRICO 653 $aGRAFICAS AGROCLIMATICOS 653 $aGRAS 653 $aHELIOFANOGRAFO 653 $aINFORMACION SATELITAL 653 $aINFORME AGROCLIMÁTICO 2019 653 $aINUNDACIONES 653 $aLLUVIAS DIARIAS 653 $aMAXIMA 653 $aMEDIA 653 $aMINIMA 653 $aPANEL SOLAR 653 $aPERSPECTIVAS CLIMATICAS 653 $aPLUVIOMETRO 653 $aPRECIPITACION NACIONAL 653 $aPREVENCION HELADAS 653 $aPRONOSTICO 653 $aSENSOR 653 $aSIMETRICO 653 $aTANQUE A 653 $aTERMOCUPLAS 653 $aTERMOHIDROGRAFO 653 $aVARIABLES AGROCLIMATICAS 653 $aVELETA 700 1 $aCAL, A. 700 1 $aTISCORNIA, G. 700 1 $aSCHIAVI, C.
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