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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
29/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GAIERO, P.; MAZZELLA, C.; VILARÓ, F.; SPERANZA, P.; DE JONG, H. |
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Afiliación : |
PAOLA GAIERO, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; Laboratory of Genetics, Wageningen University, Netherlands; CRISTINA MAZZELLA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO SPERANZA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; HANS DE JONG, Laboratory of Genetics, Wageningen University, Netherlands. |
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Título : |
Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Euphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS. |
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ISSN : |
0014-2336 |
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DOI : |
10.1007/s10681-017-1922-4 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. |
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Contenido : |
ABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato.
© 2017, The Author(s). MenosABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental spe... Presentar Todo |
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Palabras claves : |
BAC FISH; GISH; Introgressive hybridisation; Pairing behaviour; Potato wild relatives. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
https://link.springer.com/content/pdf/10.1007%2Fs10681-017-1922-4.pdf
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Marc : |
LEADER 02814naa a2200265 a 4500
001 1012827
005 2019-10-29
008 2017 bl uuuu u00u1 u #d
022 $a0014-2336
024 7 $a10.1007/s10681-017-1922-4$2DOI
100 1 $aGAIERO, P.
245 $aPairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017.
520 $aABSTRACT. Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato. © 2017, The Author(s).
653 $aBAC FISH
653 $aGISH
653 $aIntrogressive hybridisation
653 $aPairing behaviour
653 $aPotato wild relatives
700 1 $aMAZZELLA, C.
700 1 $aVILARÓ, F.
700 1 $aSPERANZA, P.
700 1 $aDE JONG, H.
773 $tEuphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS.
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INIA Las Brujas (LB) |
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| Registros recuperados : 232 | |
| 21. |  | CRISCI, C.; VILARO, F. Cosecha de papa In: CRISCI, C.; VILARO, F. Aportes tecnológicos para el cultivo de papa. Montevideo (Uruguay): INIA, 1993. p17-24 (INIA Boletin de Divulgación; 32)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 29. | | VILARÓ, F.; SUÁREZ, C. Evaluación de variedades de cebolla en la zona sur. In: INIA (Instituto Nacional de Investigación Agropecuaria); PROGRAMA HORTICULTURA. Resultados experimentales en cebolla 1993-1994. Reunión técnica. Canelones (UY): INIA Las Brujas, 1994. p. 36-39 (INIA Serie Actividades de Difusión ; 6)| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 34. | | BETTINI, R.; VILARÓ, F. Mejoramiento genético de ajo en el Uruguay. In: SUH (Sociedad Uruguay de Horticultura); COLHOR (Confederación Latinoamericana de Horticultura); Congreso Iberoamericano, 1., Congreso Latinoamericano, 5., Congreso Nacional de Horticultura, 4. Resúmenes. Montevideo (Uruguay): SUH, COLHOR, 1992. p78| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 35. | | VILARÓ, F.; SUÁREZ, C. Mejoramiento genético de ajo ln: INIA Las Brujas. Experimentos en ajo y cebolla: día de campo, 1994 Trabajos presentados. Las Brujas, Canelones (Uruguay): INIA, 1994. p. 29-39 (INIA Serie Actividades de Difusión ; 36) INIA Las Brujas| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 36. | | VILARÓ, F.; SUÁREZ, C. Mejoramiento genético de ajo. In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Programa Horticultura. Presentación de resultados experimentales en ajo y cebolla. Reunión de divulgación. Canelones (Uruguay): INIA, 2000. p. 59-61 (INIA Serie Actividades de Difusión ; 223)| Biblioteca(s): INIA Las Brujas. |
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| 38. | | VILARO, F.; SUAREZ, C. Mejoramiento genético en ajo ln: Día de campo de ajo y cebolla, 1996, Las Brujas, Canelones (UY) Memorias. Las Brujas, Canelones (Uruguay): INIA, 1996. p. 1-10 (INIA Serie Actividades de Difusión ; 117) INIA Las Brujas| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 39. | | Vilaró, F.Suárez, C. Mejoramiento genético en ajo ln: Día de campo de ajo y cebolla, 1997, Las Brujas, Canelones (UY) Memorias. Las Brujas, Canelones (Uruguay): INIA, 1997. p. 17-18 (INIA Serie Actividades de Difusión ; 151) INIA Las Brujas| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 40. | | Vilaró, F.Suárez, C. Mejoramiento genético en ajo ln: Día de campo de ajo y cebolla, 1998, Las Brujas, Canelones (UY) Memorias. Las Brujas, Canelones (Uruguay): INIA, 1998. p. 7-8 (INIA Serie Actividades de Difusión ; 177) INIA Las Brujas| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 232 | |
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