| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
26/09/2014 |
|
Actualizado : |
18/03/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
|
Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
|
Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
|
Fecha de publicación : |
2012 |
|
Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
|
ISSN : |
0567-7572 |
|
DOI : |
10.17660/ActaHortic.2012.935.8 |
|
Idioma : |
Inglés |
|
Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
|
Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
|
Thesagro : |
AJO. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 02138naa a2200229 a 4500
001 1050611
005 2022-03-18
008 2012 bl uuuu u00u1 u #d
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2012.935.8$2DOI
100 1 $aIBÁÑEZ, F.
245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource]
260 $c2012
500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding.
520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95).
650 $aAJO
700 1 $aREZANO, A.
700 1 $aFREDES, A.
700 1 $aRODRIGUEZ, G.
700 1 $aREGGIO, A.
700 1 $aVILARÓ, F.
773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 232 | |
| 167. |  | NARANCIO, R.; ZORRILLA, P.; ROBELLO, C.; GONZÁLEZ-ARCOS, M.; VILARÓ, F.; PRITSCH, C.; DALLA RIZZA, M. Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum. European Journal of Plant Pathology, 2013, v.136, no.4, p.823-835. Article history: Accepted: 27 March 2013 / Published online: 28 April 2013.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 1 |
| Biblioteca(s): INIA Las Brujas. |
|    |
| 168. | | FERREIRA, V.; PIANZZOLA, M.J.; VILARO, F.; VALLS, M.; SIRI, M.I. Nuevas herramientas para la selección y caracterización de germoplasma de papa con resistencia a marchitez bacteriana. In: JORNADA NACIONAL DE FITOPATOLOGÍA, 3; JORNADA NACIONAL DE PROTECCIÓN VEGETAL, 1., 3 SETIEMBRE 2015, MONTEVIDEO, URUGUAY. Libro de Resúmenes. Montevideo (Uruguay) : SUFIT, 2015. p. 18 Financiamiento: ANII, INIA, CAP, CSIC Grupos I+D.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 175. | | CASTILLO, A.; CEPPA, M.; DALLA RIZZA, M.; MAESO, D.; DEL PINO, G.; RODRÍGUEZ, G.; VILARÓ, F. Producción de semilla de ajo (Allium sativum L.) de sanidad mejorada. [resumen] ln: SUH (Sociedad Uruguaya de Horticultura). Congreso Nacional de Horticultura, 7.; Seminario de Liderazgo Profesional, 1., 1999, Montevideo, Uruguay Resúmenes. Montevideo (Uruguay): SUH, 1999. p. 13| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
| |
| 178. | | VAIO, M.; GAIERO, P.; VILARO, F.; SPERANZA, P.; DE JONG, H. The repetitive fraction in the genomes of wild potato relatives from Solanum section Petota. GGM 38 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. P. 273| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 179. | | GONZÁLEZ-ARCOS, M.; GALVÁN, G.; SIRI, M.I.; BORGES, A.; VILARÓ, F. Resistance to bacterial wilt in Solanum commersonii Dun. [Resistencia a la marchitez bacteriana de la papa en Solanum commersonii Dun.]. [Special Issue 25 Years Agrociencia]. Plant Biology. Agrociencia Uruguay, 2022, vol. 26, NE2, e1092. doi: https://doi.org/10.31285/AGRO.26.1092 -- OPEN ACCESS. Article history: Article originally published in: Agrociencia (Uruguay). 2013;17(1):45-54. doi: https://doi.org/10.31285/AGRO.17.513 -- Correspondence: Matías González, matgon@inia.org.uy -- Special Issue 25 Years Agrociencia. --...| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
| |
| Registros recuperados : 232 | |
|
| Expresión de búsqueda válido. Check! |
|
|
