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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
17/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. |
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Afiliación : |
VIRGINIA FERREIRA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARÍA J. PIANZZOLA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la República, Canelones, Uruguay; MARÍA L. TONDO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARÍA V. RODRÍGUEZ, Área Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; ELENA G. ORELLANO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARC VALSS, Center for Research in Agricultural Genomics, CSIC, IRTA, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain; MARÍA I. SIRI, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. |
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ISSN : |
1664-462X |
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DOI : |
10.3389/fpls.2017.01424 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. |
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Contenido : |
ABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
© 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. MenosABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typic... Presentar Todo |
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Palabras claves : |
Bacterial wilt; Disease resistance; Latent infections; Plant breeding; Potato; Ralstonia solanacearum; Solanum commersonii. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03620naa a2200337 a 4500
001 1012685
005 2019-10-17
008 2017 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2017.01424$2DOI
100 1 $aFERREIRA, V.
245 $aInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017.
520 $aABSTRACT. Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. © 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri.
653 $aBacterial wilt
653 $aDisease resistance
653 $aLatent infections
653 $aPlant breeding
653 $aPotato
653 $aRalstonia solanacearum
653 $aSolanum commersonii
700 1 $aPIANZZOLA, M.J.
700 1 $aVILARÓ, F.
700 1 $aGALVÁN, G.
700 1 $aTONDO, M.L.
700 1 $aRODRÍGUEZ, M.V.
700 1 $aORELLANO, E.G.
700 1 $aVALSS, M.
700 1 $aSIRI, M.I.
773 $tFrontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS.
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INIA Las Brujas (LB) |
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| 21. |  | CLAVIJO, F.; CURLAND, R.D.; CROCE, V.; LAPAZ, M.I.; DILL-MACKY, R.; PEREYRA, S.; SIRI, M.I. Genetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay. Phytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R Article history: Accepted for publication/ Posted online on 12 Aug 2021, First Look.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 23. | | DENIS, N.; FERREIRA, V.; RODRÍGUEZ, G.; NÚÑEZ, N.; VILARÓ, F.; VALLE, D.; GAIERO, P.; SIRI, M.I. O8. Caracterización de resistencia a sarna en germoplasma de papa y selección de genotipos contrastantes para análisis metagenómico en la geocaulósfera. [Presentación oral]. Presentaciones orales. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 22. Financiamiento: Proyecto CSIC Grupos I+D 2019-2023: "Bacterias fitopatógenas: mecanismos de resistencia hospedera y de interacción planta patógeno". Proyecto CSIC Grupos I+D 2023-2027: "Estudios integrados para el manejo de patógenos...| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 24. | | DE ARMAS, S.; DENIS, N.; SHIN, G.Y.; KVITKO, B.; VICENTE, E.; GALVÁN, G.; SIRI, M.I. Identificación y caracterización genómica de especies de pantoea asociadas a pudriciones de bulbos y lesiones foliares en cultivos de cebolla en Uruguay. 305. (resúmen). Áreas temáticas: Microbiología. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.202. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 25. | | FORT, S.; FERREIRA, V.; MURCHIO, S.; SCHVARTZMAN, C.; GALVÁN, G.A.; VILARÓ, F.; SIRI, M.I.; DALLA RIZZA, M. Potato plants transformed with the Arabidopsis EF-Tu receptor (EFR) show restricted pathogen colonization and enhanced bacterial with resistance under conditions resembling natural field infections. [Plantas de papa transformadas con el receptor de EF-Tu (EFR) de Arabidopsis presentan una colonización restringida del patógeno y mayor resistencia a la marchitez Bacteriana bajo condiciones semejantes a la infección natural de campo]. Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. Agrociencia Uruguay 2020, v. 24, no. NE2, Article 413. DOI: 10.31285/AGRO.24.413 16 p. 2301-1548 Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020.
Comité científico editor:
Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : -- - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| 26. | | DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| 27. | | VIERA, L.; ALCOBA, F.; GONZÁLEZ-BARRIOS, P.; RODRÍGUEZ, G.; GONZÁLEZ-ARCOS, M.; FERREIRA, V.; GALVÁN, G.; SIRI, M.I.; GAIERO, P.; VILARÓ, F. Evaluación de la resistencia a marchitez bacteriana en germoplasma avanzado de Papa (Solanum tuberosum). 153. (resúmen). Áreas temáticas: Genética. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.151-152. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 28. | | FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 29. | | SIRI, M.I.; CROCE, V.; LAPAZ, M.I.; HERNÁNDEZ, F.; MONTELONGO, M.J.; GONZÁLEZ-ARCOS, M.; MAESO, D.; PIANZZOLA, M.J. Identificación, caracterización y diagnóstico molecular de bacterias patógenas que afecta a los cultivos de tomate. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Hortícola. Resultados experimentales en sanidad de tomate y morrón. Jornada de Divulgación. Canelones (Uruguay): INIA, 2015. p. 3-16 (Serie Actividades de Difusión ; 756).| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 30. | | BOSCHI, F.; SCHVARTZMAN, C.; MURCHIO, S.; FERREIRA, V.; SIRI, M.I.; GALVÁN, G.; SMOKER, M.; STRANSFEL, L.; ZIPFEL, C.; VILARÓ, F.; DALLA RIZZA, M. Resistencia a marchitez bacteriana en papa mediada por expression de EFR e introgression de genes de resistencia de Solanum commersonii. In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, X. Encuentro Nacional de REDBIO, II. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2017. p.15-18. (Serie Actividades de Difusión; 780)| Biblioteca(s): INIA Las Brujas. |
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| 31. | | FERREIRA, V.; TOURNÉ, F.; EASTMAN, I.; RODRÍGUEZ-ESPERÓN, C.; RODRÍGUEZ, G.; GONZÁLEZ-ARCOS, M.; MURCHIO, S.; DALLA RIZZA, M.; VILARÓ, F.; GALVÁN, G.A.; GAIERO, P.; LARAMA, G.; GONZÁLEZ, M.; PLATERO, R.; SIRI. M.I. P22. Análisis de la comunidad bacteriana de la rizósfera de genotipos de papa con diferentes niveles de resistencia a la marchitez bacteriana. [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 48. Financiamiento: Proyecto Fondo Vaz Ferreira (DICYT): "Microbioma y resistencia: nuevo eje de investigación en el estudio de la marchitez bacteriana de la papa causada por Ralstonia solanacearum". -- Autor correspondencia: e-mail:...| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 32. | | GAIERO, P.; ANDINO, M.; VAIO, M.; VIDAL, R.; ABAD-NJERS, G.; AMARILLO, A.; SILVA, S.; HERNÁNDEZ, N.; RAMOS, S.; STANCOV, V.; MOREIRA, L.; HEIDEN, G.; NICOLAO, R.; TORANZA, C.; CASTILLO, A.; IBÁÑEZ, F.; RODRÍGUEZ, G.; GONZÁLEZ-ARCOS, M.; GALVÁN, G.; SIRI, M.I.; VILARÓ, F.; SPERANZA, P. Identificación de grupos genéticos y distribución de la variabilidad de papas silvestres para su conservación en colecciones núcleo y uso en mejoramiento genético. [Resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Hortícola. Resúmenes. Jornada Mejoramiento Genético de Hortalizas: Ciencia y Tecnología para la producción y el consumidor, 2019, Salto, Uruguay. Trabajos de investigación relacionados al proyecto. Salto (UY): INIA, 2019. p. 40-41.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 32 | |
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