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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
17/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. |
Afiliación : |
VIRGINIA FERREIRA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARÍA J. PIANZZOLA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la República, Canelones, Uruguay; MARÍA L. TONDO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARÍA V. RODRÍGUEZ, Área Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; ELENA G. ORELLANO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARC VALSS, Center for Research in Agricultural Genomics, CSIC, IRTA, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain; MARÍA I. SIRI, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
Título : |
Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. |
ISSN : |
1664-462X |
DOI : |
10.3389/fpls.2017.01424 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. |
Contenido : |
ABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
© 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. MenosABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typic... Presentar Todo |
Palabras claves : |
Bacterial wilt; Disease resistance; Latent infections; Plant breeding; Potato; Ralstonia solanacearum; Solanum commersonii. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 03620naa a2200337 a 4500 001 1012685 005 2019-10-17 008 2017 bl uuuu u00u1 u #d 022 $a1664-462X 024 7 $a10.3389/fpls.2017.01424$2DOI 100 1 $aFERREIRA, V. 245 $aInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. 520 $aABSTRACT. Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. © 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. 653 $aBacterial wilt 653 $aDisease resistance 653 $aLatent infections 653 $aPlant breeding 653 $aPotato 653 $aRalstonia solanacearum 653 $aSolanum commersonii 700 1 $aPIANZZOLA, M.J. 700 1 $aVILARÓ, F. 700 1 $aGALVÁN, G. 700 1 $aTONDO, M.L. 700 1 $aRODRÍGUEZ, M.V. 700 1 $aORELLANO, E.G. 700 1 $aVALSS, M. 700 1 $aSIRI, M.I. 773 $tFrontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS.
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
10/09/2020 |
Actualizado : |
10/09/2020 |
Tipo de producción científica : |
Informes Agroclimáticos |
Autor : |
INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA); GRAS |
Afiliación : |
UNIDAD DE AGROCLIMA Y SISTEMAS DE INFORMACIÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Informe agroclimático 2020- Situación a Agosto. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Montevideo (Uruguay): INIA, 2020. |
Páginas : |
5 p. |
Serie : |
(Informe Agroclimático; Año 15, No.8) |
Idioma : |
Español |
Notas : |
Equipo de trabajo INIA-GRAS (Unidad de Agtech y sistemas de Información): Adrián Cal, Guadalupe Tiscornia, Carlos Schiavi, Gabriel García. |
Contenido : |
Contenido. Síntesis de la Situación Agroclimática de Agosto-- Perspectivas Climáticas Trimestrales elaboradas por el IRI de la Universidad de Columbia -- Índice de Vegetación (IVDN) -- Precipitaciones -- Porcentaje de Agua Disponible (PAD) -- Índice de Bienestar Hídrico (IBH) -- Agua No Retenida (ANR) -- Perspectivas Climáticas Set-Oct-Nov elaboradas por el IRI de la Universidad de Columbia. Destacamos para este mes: Pronóstico de Don en trigo y condiciones favorables para el
desarrollo de fusariosis de espiga en trigo. Link directo: www.inia.uy/gras/Alertas-y-herramientas/Pron%C3%B3stico-DON-para-trigo- |
Palabras claves : |
AGROCLIMA; AGROCLIMATOLOGÍA; AGTECH; BOLETIN AGROCLIMÁTICO; CARACTERIZACIÓN AGROCLIMÁTICA; DIRECCION VIENTO; ESTACIONES AGROMETEOROLOGICAS; ESTACIONES AUTOMATICAS; ESTACIONES INIA; ESTADO DEL TIEMPO; ESTRÉS HÍDRICO; GRAFICAS AGROCLIMATICAS; GRAS; HELIOFANOGRAFO; INFORMACION SATELITAL; INFORME AGROCLIMÁTICO 2020; INUNDACIONES; LLUVIAS DIARIAS; MAXIMA; MEDIA; MINIMA; PANEL SOLAR; PERSPECTIVAS CLIMATICAS; PLUVIOMETRO; PRECIPITACION NACIONAL; PREVENCION HELADAS; PRONOSTICO; SENSOR; SIMETRICO; TANQUE A; TERMOCUPLAS; TERMOHIDROGRAFO; VARIABLES AGROCLIMATICAS; VELETA. |
Thesagro : |
AGROCLIMATOLOGIA; CAMBIO CLIMATICO; CLIMA; CLIMATOLOGIA; ESTACIONES METEOROLOGICAS; ESTRES HIDRICO; EVAPORACION; EVAPOTRANSPIRACION; HUMEDAD; HUMEDAD RELATIVA; LLUVIA; METEOROLOGIA; PERSPECTIVAS; PLUVIOMETROS; PRONOSTICO DEL TIEMPO; SENSORES; SISTEMAS; SISTEMAS DE INFORMACION; SUELO; TEMPERATURA; TERMOMETROS. |
Asunto categoría : |
P40 Meteorología y climatología |
URL : |
http://www.inia.uy/Publicaciones/Documentos%20compartidos/Informe%20agroclimatico%20INIA-GRAS%20Agosto%20de%202020.pdf
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Marc : |
LEADER 02938nam a2200817 a 4500 001 1061307 005 2020-09-10 008 2020 bl uuuu u0uu1 u #d 100 1 $aINIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA) 245 $aInforme agroclimático 2020- Situación a Agosto.$h[electronic resource] 260 $aMontevideo (Uruguay): INIA$c2020 300 $a5 p. 490 $a(Informe Agroclimático; Año 15, No.8) 500 $aEquipo de trabajo INIA-GRAS (Unidad de Agtech y sistemas de Información): Adrián Cal, Guadalupe Tiscornia, Carlos Schiavi, Gabriel García. 520 $aContenido. Síntesis de la Situación Agroclimática de Agosto-- Perspectivas Climáticas Trimestrales elaboradas por el IRI de la Universidad de Columbia -- Índice de Vegetación (IVDN) -- Precipitaciones -- Porcentaje de Agua Disponible (PAD) -- Índice de Bienestar Hídrico (IBH) -- Agua No Retenida (ANR) -- Perspectivas Climáticas Set-Oct-Nov elaboradas por el IRI de la Universidad de Columbia. Destacamos para este mes: Pronóstico de Don en trigo y condiciones favorables para el desarrollo de fusariosis de espiga en trigo. Link directo: www.inia.uy/gras/Alertas-y-herramientas/Pron%C3%B3stico-DON-para-trigo- 650 $aAGROCLIMATOLOGIA 650 $aCAMBIO CLIMATICO 650 $aCLIMA 650 $aCLIMATOLOGIA 650 $aESTACIONES METEOROLOGICAS 650 $aESTRES HIDRICO 650 $aEVAPORACION 650 $aEVAPOTRANSPIRACION 650 $aHUMEDAD 650 $aHUMEDAD RELATIVA 650 $aLLUVIA 650 $aMETEOROLOGIA 650 $aPERSPECTIVAS 650 $aPLUVIOMETROS 650 $aPRONOSTICO DEL TIEMPO 650 $aSENSORES 650 $aSISTEMAS 650 $aSISTEMAS DE INFORMACION 650 $aSUELO 650 $aTEMPERATURA 650 $aTERMOMETROS 653 $aAGROCLIMA 653 $aAGROCLIMATOLOGÍA 653 $aAGTECH 653 $aBOLETIN AGROCLIMÁTICO 653 $aCARACTERIZACIÓN AGROCLIMÁTICA 653 $aDIRECCION VIENTO 653 $aESTACIONES AGROMETEOROLOGICAS 653 $aESTACIONES AUTOMATICAS 653 $aESTACIONES INIA 653 $aESTADO DEL TIEMPO 653 $aESTRÉS HÍDRICO 653 $aGRAFICAS AGROCLIMATICAS 653 $aGRAS 653 $aHELIOFANOGRAFO 653 $aINFORMACION SATELITAL 653 $aINFORME AGROCLIMÁTICO 2020 653 $aINUNDACIONES 653 $aLLUVIAS DIARIAS 653 $aMAXIMA 653 $aMEDIA 653 $aMINIMA 653 $aPANEL SOLAR 653 $aPERSPECTIVAS CLIMATICAS 653 $aPLUVIOMETRO 653 $aPRECIPITACION NACIONAL 653 $aPREVENCION HELADAS 653 $aPRONOSTICO 653 $aSENSOR 653 $aSIMETRICO 653 $aTANQUE A 653 $aTERMOCUPLAS 653 $aTERMOHIDROGRAFO 653 $aVARIABLES AGROCLIMATICAS 653 $aVELETA 700 1 $aGRAS
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