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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
27/01/2020 |
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Actualizado : |
27/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ESCRIBANO, C.; SARAVIA, A.; COSTA, M.; CASTELLS, D.; CIAPPESONI, G.; RIET-CORREA, F.; FREIRE, T. |
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Afiliación : |
CECILIA ESCRIBANO, Grupo de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDERSON SARAVIA DE MELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONIQUE COSTA, Grupo de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay; DANIEL CASTELLS, Secretariado Uruguayo de Lana, Florida, Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; TERESA FREIRE, Grupo de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay. |
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Título : |
Resistance to Haemonchus contortus in Corriedale sheep is associated to high parasite-specific IgA titer and a systemic Th2 immune response. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Scientific Reports, 1 December 2019, Volume 9, Issue 1, Article number 19579. OPEN ACCESS. Doi: https://doi.org/10.1038/s41598-019-55447-6 |
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ISSN : |
2045-2322 |
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DOI : |
10.1038/s41598-019-55447-6 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 21 June 2019 / Accepted: 27 November 2019 / Published: 20 December 2019.
Funding information:
We acknowledge the personnel from Uruguayan Wool Secretariat that assisted with animal studies. This project was funded by INIA (Uruguay) and CSIC-VUSP (Uruguay). C.E. was funded by a scholarship by INIA. |
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Contenido : |
ABSTRACT.
Gastrointestinal nematode infections, including Haemonchus contortus, are one of the main causes of economic losses to ovine farmers worldwide. In order to contribute to the control of nematode infections and avoid parasite spreading we generated divergent resistant and susceptible sheep breeds and evaluated the adaptive immunity of these animals developed upon experimental infection against H. contortus. The selection of resistant or susceptible animals from the Corriedale Breed has been based on Expected Progeny Differences for faecal egg counts per gram. Furthermore, animals from the resistant Corriedale line were inseminated with imported semen from Australian Rylington Merino rams. Thus, the objective of this work was to analyze the adaptive immune response in both susceptible and resistant obtained lambs. Our results indicate that there is a potent parasite-specific local and systemic immune response in resistant animals and that although susceptible lambs can produce high levels of IgA antibodies during the infection, their antibody response is delayed which, together with an impaired specific-Th2 response, does not contribute to initial parasite elimination. Our results shed light into the immune mechanisms that mediate resistance to H. contortus and could constitute important assets to sheep farmers, not only as a means to detect resistance, but also to enhance the efficiency of selection in stud flocks.
© 2019, The Author(s). |
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Palabras claves : |
HAEMONCHUS; SHEEP. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.nature.com/articles/s41598-019-55447-6.pdf
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Marc : |
LEADER 02635naa a2200253 a 4500
001 1060699
005 2020-01-27
008 2019 bl uuuu u00u1 u #d
022 $a2045-2322
024 7 $a10.1038/s41598-019-55447-6$2DOI
100 1 $aESCRIBANO, C.
245 $aResistance to Haemonchus contortus in Corriedale sheep is associated to high parasite-specific IgA titer and a systemic Th2 immune response.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 21 June 2019 / Accepted: 27 November 2019 / Published: 20 December 2019. Funding information: We acknowledge the personnel from Uruguayan Wool Secretariat that assisted with animal studies. This project was funded by INIA (Uruguay) and CSIC-VUSP (Uruguay). C.E. was funded by a scholarship by INIA.
520 $aABSTRACT. Gastrointestinal nematode infections, including Haemonchus contortus, are one of the main causes of economic losses to ovine farmers worldwide. In order to contribute to the control of nematode infections and avoid parasite spreading we generated divergent resistant and susceptible sheep breeds and evaluated the adaptive immunity of these animals developed upon experimental infection against H. contortus. The selection of resistant or susceptible animals from the Corriedale Breed has been based on Expected Progeny Differences for faecal egg counts per gram. Furthermore, animals from the resistant Corriedale line were inseminated with imported semen from Australian Rylington Merino rams. Thus, the objective of this work was to analyze the adaptive immune response in both susceptible and resistant obtained lambs. Our results indicate that there is a potent parasite-specific local and systemic immune response in resistant animals and that although susceptible lambs can produce high levels of IgA antibodies during the infection, their antibody response is delayed which, together with an impaired specific-Th2 response, does not contribute to initial parasite elimination. Our results shed light into the immune mechanisms that mediate resistance to H. contortus and could constitute important assets to sheep farmers, not only as a means to detect resistance, but also to enhance the efficiency of selection in stud flocks. © 2019, The Author(s).
653 $aHAEMONCHUS
653 $aSHEEP
700 1 $aSARAVIA, A.
700 1 $aCOSTA, M.
700 1 $aCASTELLS, D.
700 1 $aCIAPPESONI, G.
700 1 $aRIET-CORREA, F.
700 1 $aFREIRE, T.
773 $tScientific Reports, 1 December 2019, Volume 9, Issue 1, Article number 19579. OPEN ACCESS. Doi: https://doi.org/10.1038/s41598-019-55447-6
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
16/08/2019 |
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Actualizado : |
16/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
HERNÁNDEZ-RODRÍGUEZ, L.; RAMOS-GONZÁLEZ, P. L.; SISTACHS-VEGA, V.; ZAMORA-RODRÍGUEZ, V.; BATISTA-LE RIVEREND, L.; RAMOS-LEAL, M.; PEÑA-BÁRZAGA, I.; LLANES-ALVAREZ, Y. |
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Afiliación : |
LESTER HERNÁNDEZ RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; P. L. RAMOS GONZÁLEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba; Instituto Biológico de São Paulo, Brazil.; V. SISTACHS-VEGA, Statistics Department, Faculty of Mathematics, Universidad de La Habana, Cuba.; V. ZAMORA-RODRI?GUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; L. BATISTA-LE RIVEREND, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba; M. RAMOS-LEAL, Department, Faculty of Mathematics, Universidad de La Habana, Cuba.; I. PEN?A-BA?RZAGA, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; Y. LLANES-ALVAREZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. |
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Título : |
The viral complex associated with mealybug wilt disease of pineapple in Cuba. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Acta Horticulturae, 30 April 2019, Volume 1239, pages 203-212. |
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Serie : |
(Acta Horticulturae; 1239). |
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ISBN : |
978-94-62612-36-5 |
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ISSN : |
0567-7572 (print) // 2406-6168 (electronic) |
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DOI : |
10.17660/ActaHortic.2019.1239.25 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 30 april 2019. In: Acta Horticulturae (ISHS) 1239: IX International Pineapple Symposium. Editors: D.P. Bartholomew, D.H. Reinhardt, F.V. Duarte Souza. Convener: J. Valdés-Infante Herrero .
Funding text: Part of these results are included in the Ph.D. thesis document of Lester Hernández-Rodrı́guez, |
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Contenido : |
ABSTRACT.
Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease.
© 2019 International Society for Horticultural Science. All rights reserved. MenosABSTRACT.
Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease.
© 2019 International Society for Horticultural Science. ... Presentar Todo |
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Palabras claves : |
AMPELOVIRUS; ANANAS COMOSUS; BADNAVIRUS; CAULIMOVIRIDAE; CLOSTEROVIRIDAE; MWP; PBCOV; PMWaV. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02929naa a2200361 a 4500
001 1060012
005 2019-08-16
008 2019 bl uuuu u00u1 u #d
020 $a978-94-62612-36-5
022 $a0567-7572 (print) // 2406-6168 (electronic)
024 7 $a10.17660/ActaHortic.2019.1239.25$2DOI
100 1 $aHERNÁNDEZ-RODRÍGUEZ, L.
245 $aThe viral complex associated with mealybug wilt disease of pineapple in Cuba.$h[electronic resource]
260 $c2019
490 $a(Acta Horticulturae; 1239).
500 $aArticle history: Published 30 april 2019. In: Acta Horticulturae (ISHS) 1239: IX International Pineapple Symposium. Editors: D.P. Bartholomew, D.H. Reinhardt, F.V. Duarte Souza. Convener: J. Valdés-Infante Herrero . Funding text: Part of these results are included in the Ph.D. thesis document of Lester Hernández-Rodrı́guez,
520 $aABSTRACT. Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease. © 2019 International Society for Horticultural Science. All rights reserved.
653 $aAMPELOVIRUS
653 $aANANAS COMOSUS
653 $aBADNAVIRUS
653 $aCAULIMOVIRIDAE
653 $aCLOSTEROVIRIDAE
653 $aMWP
653 $aPBCOV
653 $aPMWaV
700 1 $aRAMOS-GONZÁLEZ, P. L.
700 1 $aSISTACHS-VEGA, V.
700 1 $aZAMORA-RODRÍGUEZ, V.
700 1 $aBATISTA-LE RIVEREND, L.
700 1 $aRAMOS-LEAL, M.
700 1 $aPEÑA-BÁRZAGA, I.
700 1 $aLLANES-ALVAREZ, Y.
773 $tActa Horticulturae, 30 April 2019, Volume 1239, pages 203-212.
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