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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
03/02/2020 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MONTENEGRO, M.C.; PERAZA, P.; BALEMIAN, N.; CARBALLO, C.; BARLOCCO, N.; BARRIOS, P.G.; MERNIES, B.; SAADOUN, A.; CASTRO, G.; GUIMARÃES, S.F.; LLAMBÍ, S. |
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Afiliación : |
M. C. MONTENEGRO, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; PABLO PERAZA DOS SANTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. BALEMIAN, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; C. CARBALLO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; N. BARLOCCO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; P. G. BARRIOS, Departamento de Biometría, Estadística y Cómputo, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; B. MERNIES, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; A. SAADOUN, Sección Fisiología y Nutrición, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; G. CASTRO, Departamento Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; S.F. GUIMARÃES, Departamento de Zootecnia, Universidade Federal de Viçosa, Minas Gerais, Brasil; S. LLAMBÍ, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Gene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Genetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307 |
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ISSN : |
1676-5680 |
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DOI : |
10.4238/gmr18307 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019.
Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com |
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Contenido : |
ABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits. MenosABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regu... Presentar Todo |
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Palabras claves : |
Candidate genes; Meat quality; Nutrigenomics; Skeletal muscle; Transcriptomic. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://www.geneticsmr.com/sites/default/files/articles/year2019/vol18-4/pdf/gmr18307_-_gene-expression-analysis-rna-sequencing-longissimus-dorsi-muscle-pigs-fed-diets-differing.pdf
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Marc : |
LEADER 03091naa a2200337 a 4500
001 1060743
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008 2019 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/gmr18307$2DOI
100 1 $aMONTENEGRO, M.C.
245 $aGene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents.$h[electronic resource]
260 $c2019
500 $aArticle history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019. Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com
520 $aABSTRACT. We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments. These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits.
653 $aCandidate genes
653 $aMeat quality
653 $aNutrigenomics
653 $aSkeletal muscle
653 $aTranscriptomic
700 1 $aPERAZA, P.
700 1 $aBALEMIAN, N.
700 1 $aCARBALLO, C.
700 1 $aBARLOCCO, N.
700 1 $aBARRIOS, P.G.
700 1 $aMERNIES, B.
700 1 $aSAADOUN, A.
700 1 $aCASTRO, G.
700 1 $aGUIMARÃES, S.F.
700 1 $aLLAMBÍ, S.
773 $tGenetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307
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| 3. |  | SANTOS, E.; INVERNIZZI, C.; GARCÍA, E.; CABRERA, C.; DI LANDRO, R.; SAADOUN, A.; DANERS, G. Contenido de proteína cruda del polen de las principales especies botánicas utilizadas por las abejas melíferas en Uruguay. Agrociencia, 2009, v. 13, no. 2, p. 9-13| Biblioteca(s): INIA Tacuarembó. |
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| 4. | | CABRERA, M.C.; OLIVERO, R.; DEL PUERTO, M.; SAADOUN, A.; NAYA, D.; ZUNINI, P.; GUERRA, H. Características digestivas de la nutria en la etapa de engorde y su importancia en la regulación de la ingesta de alimento, utilización de la proteína, fibra y energía alimentaria Las Brujas, Canelones (Uruguay): INIA, 2002. p. 1-3 (INIA Serie Actividades de Difusión ; 290)| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas. |
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| 8. | | ROVIRA, P.J.; BRUGNINI , G.; RODRIGUEZ, J.; CABRERA, M.C.; SAADOUN, A.; DE SOUZA, G.; LUZARDO, S.; RUFO, C. Microbiological changes during long-storage of beef meat under different temperature and vacuum-packaging conditions. Foods, 2023, volume 12, issue 4, article 694. OPEN ACCESS. doi: https://doi.org/10.3390/foods12040694 Article history: Received 28 December 2022; Revised 26 January 2023; Accepted 30 January 2023; Published 6 February 2023. -- Correspondence: Santiago Luzardo, mail: sluzardo@inia.org.uy (S.L.); Caterina Rufo, mail: crufo@fq.edu.uy (C.R.)...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 9. | | LUZARDO, S.; SAADOUN, A.; CABRERA, M. C.; TEREVINTO, A.; BRUGNINI, G.; RODRIGUEZ, J.; DE SOUZA, G.; ROVIRA, P.J.; RUFO, C. Effect of beef long-storage under different temperatures and vacuum-packaging conditions on meat quality, oxidation processes and microbial growth. Journal of the Science of Food and Agriculture, 2023. https://doi.org/10.1002/jsfa.12999 -- OPEN ACCESS. Article history: Manuscript received 04 June 2023, Manuscript revised 09 August 2023, Manuscript accepted 19 September 2023, Accepted manuscript online 22 September 2023, Version of Record online 06 October 2023. -- Correspondence...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 10. | | MONTENEGRO, M.C.; PERAZA, P.; BALEMIAN, N.; CARBALLO, C.; BARLOCCO, N.; BARRIOS, P.G.; MERNIES, B.; SAADOUN, A.; CASTRO, G.; GUIMARÃES, S.F.; LLAMBÍ, S. Gene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents. Genetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307 Article history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019.
Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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