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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
04/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RAVAGNOLO, O.; MISZTAL, I. |
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Afiliación : |
OLGA RAVAGNOLO GUMILA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; I. MISZTAL, Dept. of Animal and Dairy Science, University of Georgia, Athens, GA, United States. |
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Título : |
Studies on genetics of heat tolerance in dairy cattle with reduced weather information via cluster analysis. |
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Fecha de publicación : |
2002 |
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Fuente / Imprenta : |
Journal of Dairy Science, June 2002, Volume 85, Issue 6, Pages 1586-1589. OPEN ACCESS. DOI: https://doi.org/10.3168/jds.S0022-0302(02)74228-8 |
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ISSN : |
0022-0302 |
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DOI : |
10.3168/jds.S0022-0302(02)74228-8 |
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Idioma : |
Inglés |
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Notas : |
Article history: |
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Contenido : |
ABSTRACT.
The objective of this study was to explore the possibility of reducing the number of weather stations for studies on genetics of heat tolerance in dairy cattle. The similarity of information from 21 Georgia weather stations was analyzed by cluster analysis. Two major clusters have been found, separating Georgia along the NE and SW line. One weather station was selected for each of the clusters based on the minimal distance to all the remaining weather stations and on completeness of the weather information. The production dataset consisted of 114,751 first-parity test-day records for milk on 14,297 Holsteins from 120 herds in Georgia. Analyses using a model for daily milk yield with temperature-humidity index classes and several other fixed effects showed no increase in error sum of squares when using only two weather stations. The threshold of heat stress was different for each of the two regions but the rate of decline after the threshold was similar. After accounting for different thresholds, the genetic component of heat tolerance for milk was higher with the two-station model. Genetic studies on or evaluation for heat tolerance based on information from a few carefully selected weather stations can be as accurate as those based on information from numerous such stations. |
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Palabras claves : |
Dairy; Heat stress; Random regressions. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.journalofdairyscience.org/article/S0022-0302(02)74228-8/pdf
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Marc : |
LEADER 02029naa a2200205 a 4500
001 1012856
005 2020-02-04
008 2002 bl uuuu u00u1 u #d
022 $a0022-0302
024 7 $a10.3168/jds.S0022-0302(02)74228-8$2DOI
100 1 $aRAVAGNOLO, O.
245 $aStudies on genetics of heat tolerance in dairy cattle with reduced weather information via cluster analysis.$h[electronic resource]
260 $c2002
500 $aArticle history:
520 $aABSTRACT. The objective of this study was to explore the possibility of reducing the number of weather stations for studies on genetics of heat tolerance in dairy cattle. The similarity of information from 21 Georgia weather stations was analyzed by cluster analysis. Two major clusters have been found, separating Georgia along the NE and SW line. One weather station was selected for each of the clusters based on the minimal distance to all the remaining weather stations and on completeness of the weather information. The production dataset consisted of 114,751 first-parity test-day records for milk on 14,297 Holsteins from 120 herds in Georgia. Analyses using a model for daily milk yield with temperature-humidity index classes and several other fixed effects showed no increase in error sum of squares when using only two weather stations. The threshold of heat stress was different for each of the two regions but the rate of decline after the threshold was similar. After accounting for different thresholds, the genetic component of heat tolerance for milk was higher with the two-station model. Genetic studies on or evaluation for heat tolerance based on information from a few carefully selected weather stations can be as accurate as those based on information from numerous such stations.
653 $aDairy
653 $aHeat stress
653 $aRandom regressions
700 1 $aMISZTAL, I.
773 $tJournal of Dairy Science, June 2002, Volume 85, Issue 6, Pages 1586-1589. OPEN ACCESS. DOI: https://doi.org/10.3168/jds.S0022-0302(02)74228-8
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Registro original : |
INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
30/06/2021 |
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Actualizado : |
30/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. |
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Afiliación : |
ELEONOR CASTRO JANER, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; ANDRÉ DÍAZ, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA FONTES, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA BARAIBAR, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; TATIANA SAPORITI NOGUEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA ERNESTINA OLHAGARAY, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay. |
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Título : |
Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 |
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ISSN : |
1877-959X |
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DOI : |
10.1016/j.ttbdis.2021.101747 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35).
Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com |
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Contenido : |
ABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 MenosABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals an... Presentar Todo |
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Palabras claves : |
Acaricides; Ixodidae; Reaxys retrieves substances; Rhipicephalus microplus. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 03773naa a2200265 a 4500
001 1062207
005 2021-06-30
008 2021 bl uuuu u00u1 u #d
022 $a1877-959X
024 7 $a10.1016/j.ttbdis.2021.101747$2DOI
100 1 $aCASTRO JANER, E.
245 $aMolecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021. The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35). Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com
520 $aABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021
653 $aAcaricides
653 $aIxodidae
653 $aReaxys retrieves substances
653 $aRhipicephalus microplus
700 1 $aDÍAZ, A.
700 1 $aFONTES, F.
700 1 $aBARAIBAR, F.
700 1 $aSAPORITI, T.
700 1 $aOLHAGARAY, M. E.
773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747
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