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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
19/10/2023 |
Actualizado : |
19/10/2023 |
Tipo de producción científica : |
Poster |
Autor : |
GIAMBIASI, M.; BRITOS, A.; CASTILLO, A.; BERTALMIO, A.; ROLON, R.; BERTONI, E.; GONCALVEZ, L.; LAXAGUE, J.; ADALID, E.; RIVAS, F. |
Afiliación : |
MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LUCIA BRITOS SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO ROQUE ROLON RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ESTEFANY BERTONI CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LUCIA GONCALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE IGNACIO LAXAGUE BARNECHE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELBIO ADALID GUTIÉRREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Obtención de poliploides de mandarinas para la producción de frutas sin semillas. [poster]. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
In: IX Congreso Argentino de Citricultura, Tucumán (Argentina), 11 - 14 de junio 2019. |
Idioma : |
Español |
Contenido : |
La producción de cítricos de Uruguay está orientada al consumo en fresco y exportación a mercados exigentes como EEUU y Europa. La presencia de semillas en los frutos es un factor que limita la competitividad al causar una importante desvalorización del producto. El Programa de Mejoramiento Genético de Citrus de Uruguay ha orientado esfuerzos hacia la obtención de variedades de mandarinas triploides (3x) las cuales no producen semillas. Para ello es necesario realizar cruzamientos interploides entre plantas tetraploides (4x) y diploides (2x), con el posterior rescate de embriones 3x mediante cultivo in vitro. El objetivo del trabajo fue obtener genotipos parentales 4x para la producción de genotipos 3x originales. |
Thesagro : |
CITRUS; MANDARINAS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17392/1/Giambiasi-etal-2019-IXCongresoCitricultura-poster.pdf
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Marc : |
LEADER 01444nam a2200241 a 4500 001 1064349 005 2023-10-19 008 2019 bl uuuu u00u1 u #d 100 1 $aGIAMBIASI, M. 245 $aObtención de poliploides de mandarinas para la producción de frutas sin semillas. [poster].$h[electronic resource] 260 $aIn: IX Congreso Argentino de Citricultura, Tucumán (Argentina), 11 - 14 de junio 2019.$c2019 520 $aLa producción de cítricos de Uruguay está orientada al consumo en fresco y exportación a mercados exigentes como EEUU y Europa. La presencia de semillas en los frutos es un factor que limita la competitividad al causar una importante desvalorización del producto. El Programa de Mejoramiento Genético de Citrus de Uruguay ha orientado esfuerzos hacia la obtención de variedades de mandarinas triploides (3x) las cuales no producen semillas. Para ello es necesario realizar cruzamientos interploides entre plantas tetraploides (4x) y diploides (2x), con el posterior rescate de embriones 3x mediante cultivo in vitro. El objetivo del trabajo fue obtener genotipos parentales 4x para la producción de genotipos 3x originales. 650 $aCITRUS 650 $aMANDARINAS 700 1 $aBRITOS, A. 700 1 $aCASTILLO, A. 700 1 $aBERTALMIO, A. 700 1 $aROLON, R. 700 1 $aBERTONI, E. 700 1 $aGONCALVEZ, L. 700 1 $aLAXAGUE, J. 700 1 $aADALID, E. 700 1 $aRIVAS, F.
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
19/04/2023 |
Actualizado : |
19/04/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
BOK, M.; VEGA, C.G.; CASTELLS, M.; COLINA, R.; WIGDOROVITZ, A.; PARREÑO, V. |
Afiliación : |
MARINA BOK, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina; CELINA G. VEGA, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina; MATÍAS CASTELLS BAUER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Molecular Virology Laboratory, CENUR North Litoral, Salto University Centre, University of the Republic, Salto, 50000, Uruguay; RODNEY COLINA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Molecular Virology Laboratory, CENUR North Litoral, Salto University Centre, University of the Republic, Salto, 50000, Uruguay; ANDRÉS WIGDOROVITZ, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina; VIVIANA PARREÑO, INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, 1686, Argentina; CONICET, Buenos Aires, C1425FQB, Argentina; Bioinnovo S.A, Buenos Aires, 1686, Argentina. |
Título : |
Development of an IgY-based treatment to control bovine coronavirus diarrhea in dairy calves. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Viruses, 2023, volume 15, issue 3, article 708. OPEN ACCESS. doi: https://doi.org/10.3390/v15030708 |
ISSN : |
1999-4915 |
DOI : |
10.3390/v15030708 |
Idioma : |
Inglés |
Notas : |
Article history: Received 6 February 2023; Revised 5 March 2023; Accepted 6 March 2023; Published 9 March 2023. -- Academic Editor: Tomomi Takano. -- Correspondence author: Bok, M.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires 1686, Argentina; email:bok.marina@inta.gob.ar ; Parreño, V.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, Argentina; email:parreno.viviana@inta.gob.ar -- This article belongs to the Special Issue Animal Coronaviruses: Infection, Prevention, and Antivirals https://www.mdpi.com/journal/viruses/special_issues/5XDFOYZ724 ) -- Funding: This work was supported by The National Institute of Agricultural Technology (INTA), ANPCyT from the Argentinean Agricultural Ministry (PICT Start Up 2010-1532), and Vetanco S.A. (CVT 20794). -- |
Contenido : |
Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ELISA = 512; VN = 32) for 14 days as a passive treatment before a challenge with BCoV and were compared to calves fed milk with no supplementation. This is the first study with proof of efficacy of a product based on egg powder manufactured at a scale that successfully prevents BCoV-associated neonatal calf diarrhea. © 2023 by the authors. MenosBovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ... Presentar Todo |
Palabras claves : |
Bovine coronavirus; Development; ELISA validation; IgY antibodies; Neonatal calf diarrhea. |
Asunto categoría : |
L20 Ecología animal |
URL : |
https://www.mdpi.com/1999-4915/15/3/708/pdf
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Marc : |
LEADER 03567naa a2200277 a 4500 001 1064043 005 2023-04-19 008 2023 bl uuuu u00u1 u #d 022 $a1999-4915 024 7 $a10.3390/v15030708$2DOI 100 1 $aBOK, M. 245 $aDevelopment of an IgY-based treatment to control bovine coronavirus diarrhea in dairy calves.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 6 February 2023; Revised 5 March 2023; Accepted 6 March 2023; Published 9 March 2023. -- Academic Editor: Tomomi Takano. -- Correspondence author: Bok, M.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires 1686, Argentina; email:bok.marina@inta.gob.ar ; Parreño, V.; INCUINTA, Virology Institute and Technology Innovations, National Institute of Agricultural Technology, Buenos Aires, Argentina; email:parreno.viviana@inta.gob.ar -- This article belongs to the Special Issue Animal Coronaviruses: Infection, Prevention, and Antivirals https://www.mdpi.com/journal/viruses/special_issues/5XDFOYZ724 ) -- Funding: This work was supported by The National Institute of Agricultural Technology (INTA), ANPCyT from the Argentinean Agricultural Ministry (PICT Start Up 2010-1532), and Vetanco S.A. (CVT 20794). -- 520 $aBovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific antibody (Ab) titers in serum and colostrum. For the prevention to be effective, calves need to suck maternal colostrum within the first six to twelve hours of life before gut closure to ensure a good level of passive immunity. The high rate of maternal Ab transfer failure resulting from this process posed the need to develop alternative local passive immunity strategies to strengthen the prevention and treatment of BCoV diarrhea. Immunoglobulin Y technology represents a promising tool to address this gap. In this study, 200 laying hens were immunized with BCoV to obtain spray-dried egg powder enriched in specific IgY Abs to BCoV on a large production scale. To ensure batch-to-batch product consistency, a potency assay was statistically validated. With a sample size of 241, the BCoV-specific IgY ELISA showed a sensitivity and specificity of 97.7% and 98.2%, respectively. ELISA IgY Abs to BCoV correlated with virus-neutralizing Ab titers (Pearson correlation, R2 = 0.92, p < 0.001). Most importantly, a pilot efficacy study in newborn calves showed a significant delay and shorter duration of BCoV-associated diarrhea and shedding in IgY-treated colostrum-deprived calves. Calves were treated with milk supplemented with egg powder (final IgY Ab titer to BCoV ELISA = 512; VN = 32) for 14 days as a passive treatment before a challenge with BCoV and were compared to calves fed milk with no supplementation. This is the first study with proof of efficacy of a product based on egg powder manufactured at a scale that successfully prevents BCoV-associated neonatal calf diarrhea. © 2023 by the authors. 653 $aBovine coronavirus 653 $aDevelopment 653 $aELISA validation 653 $aIgY antibodies 653 $aNeonatal calf diarrhea 700 1 $aVEGA, C.G. 700 1 $aCASTELLS, M. 700 1 $aCOLINA, R. 700 1 $aWIGDOROVITZ, A. 700 1 $aPARREÑO, V. 773 $tViruses, 2023, volume 15, issue 3, article 708. OPEN ACCESS. doi: https://doi.org/10.3390/v15030708
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