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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
17/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERREIRA, V.; PIANZZOLA, M.J.; VILARÓ, F.; GALVÁN, G.; TONDO, M.L.; RODRÍGUEZ, M.V.; ORELLANO, E.G.; VALSS, M.; SIRI, M.I. |
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Afiliación : |
VIRGINIA FERREIRA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARÍA J. PIANZZOLA, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur, Facultad de Agronomía, Universidad de la República, Canelones, Uruguay; MARÍA L. TONDO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARÍA V. RODRÍGUEZ, Área Biología Vegetal (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; ELENA G. ORELLANO, Instituto de Biología Molecular y Celular de Rosario (CONICET-UNR), Rosario, Argentina; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; MARC VALSS, Center for Research in Agricultural Genomics, CSIC, IRTA, Barcelona, Spain; Department of Genetics, Universitat de Barcelona, Barcelona, Spain; MARÍA I. SIRI, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS. |
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ISSN : |
1664-462X |
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DOI : |
10.3389/fpls.2017.01424 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017. |
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Contenido : |
ABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.
© 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri. MenosABSTRACT.
Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typic... Presentar Todo |
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Palabras claves : |
Bacterial wilt; Disease resistance; Latent infections; Plant breeding; Potato; Ralstonia solanacearum; Solanum commersonii. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03620naa a2200337 a 4500
001 1012685
005 2019-10-17
008 2017 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2017.01424$2DOI
100 1 $aFERREIRA, V.
245 $aInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 30 June 2017 / Accepted: 02 August 2017 / Published: 28 August 2017.
520 $aABSTRACT. Potato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato. © 2017 Ferreira, Pianzzola, Vilaró, Galván, Tondo, Rodriguez, Orellano, Valls and Siri.
653 $aBacterial wilt
653 $aDisease resistance
653 $aLatent infections
653 $aPlant breeding
653 $aPotato
653 $aRalstonia solanacearum
653 $aSolanum commersonii
700 1 $aPIANZZOLA, M.J.
700 1 $aVILARÓ, F.
700 1 $aGALVÁN, G.
700 1 $aTONDO, M.L.
700 1 $aRODRÍGUEZ, M.V.
700 1 $aORELLANO, E.G.
700 1 $aVALSS, M.
700 1 $aSIRI, M.I.
773 $tFrontiers in Plant Science, 28 August 2017, volume 8, 1424. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/12/2023 |
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Actualizado : |
21/12/2023 |
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Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
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Autor : |
PEREYRA, S.; CASTRO, M.; VERO, S.; SILVA, P.; CAL, A.; TISCORNIA, G.; GONZALEZ, N.; BENTOS, D.; ALVAREZ, W.; RABAZA, S.; SEVILLANO, L.; BRANCATTI, G.; FRANCIA, C.; RAFFO, M.A.; GERMAN, S.; PÉREZ, C.; GARMENDIA, G.; PAREJA, L.; RODRÍGUEZ, A.; PENDAS, C.; QUINCKE, M.; VÁZQUEZ, D.; RODRIGUEZ, M. |
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Afiliación : |
SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARINA CASTRO DERENYI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVANA VERO, Facultad de Química - Udelar; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIAN TABARE CAL ALVAREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUADALUPE TISCORNIA TOSAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NESTOR RICARDO GONZALEZ PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DAHIANA BENTOS MOYANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WILLIAM HORACIO ALVAREZ FIGUEROA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SAMUEL RABAZA SOULIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEYDI GIOVANNA SEVILLANO BONILLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GIANELLA BRANCATTI, Facultad de Química - Udelar; CAROLINA FRANCIA, Facultad de Agronomía, Udelar; MIGUEL ANGEL RAFFO BUSCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS PÉREZ, Facultad de Agronomía, Udelar; GABRIELA GARMENDIA, Facultad de Química - Udelar; LUCÍA PAREJA, CENUR-Litoral Norte, Udelar; ALEJANDRA RODRÍGUEZ, IPTP, Facultad de Química, Udelar; CINTHIA PENDAS, IPTP, Facultad de Química, Udelar; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL VÁZQUEZ PEYRONEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCELO JULIAN RODRIGUEZ ALONZO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Fusariosis de la espiga en trigo y micotoxinas asociadas: contribuyendo a reducir su riesgo. |
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Complemento del título : |
Cultivos. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Revista INIA Uruguay, Diciembre 2023, no.75 p.54-58. |
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Serie : |
(Revista INIA; 75). |
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ISSN : |
1510-9011 |
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Idioma : |
Español |
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Contenido : |
Los avances de la investigación en los últimos 20 años generando conocimientos en la biología y epidemiología de la FE en nuestros sistemas de producción, en la diversidad de las poblaciones de Fusarium y sus toxinas asociadas, y herramientas disponibles para el manejo integrado, asisten en minimizar el riesgo de niveles de micotoxinas y residuos de fungicidas en los granos de trigo por encima de los permitidos. |
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Palabras claves : |
AGROALIMENTOS - INIA; SISTEMA AGRÍCOLA-GANADERO - INIA; SISTEMAS DE INFORMACIÓN Y TRANSFORMACIÓN DIGITAL - INIA. |
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Thesagro : |
FUSARIOSIS DE LA ESPIGA DE TRIGO; TRIGO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17456/1/Revista-INIA-75-dic-2023-13.pdf
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Marc : |
LEADER 01719naa a2200469 a 4500
001 1064418
005 2023-12-21
008 2023 bl uuuu u00u1 u #d
022 $a1510-9011
100 1 $aPEREYRA, S.
245 $aFusariosis de la espiga en trigo y micotoxinas asociadas$bcontribuyendo a reducir su riesgo.$h[electronic resource]
260 $c2023
490 $a(Revista INIA; 75).
520 $aLos avances de la investigación en los últimos 20 años generando conocimientos en la biología y epidemiología de la FE en nuestros sistemas de producción, en la diversidad de las poblaciones de Fusarium y sus toxinas asociadas, y herramientas disponibles para el manejo integrado, asisten en minimizar el riesgo de niveles de micotoxinas y residuos de fungicidas en los granos de trigo por encima de los permitidos.
650 $aFUSARIOSIS DE LA ESPIGA DE TRIGO
650 $aTRIGO
653 $aAGROALIMENTOS - INIA
653 $aSISTEMA AGRÍCOLA-GANADERO - INIA
653 $aSISTEMAS DE INFORMACIÓN Y TRANSFORMACIÓN DIGITAL - INIA
700 1 $aCASTRO, M.
700 1 $aVERO, S.
700 1 $aSILVA, P.
700 1 $aCAL, A.
700 1 $aTISCORNIA, G.
700 1 $aGONZALEZ, N.
700 1 $aBENTOS, D.
700 1 $aALVAREZ, W.
700 1 $aRABAZA, S.
700 1 $aSEVILLANO, L.
700 1 $aBRANCATTI, G.
700 1 $aFRANCIA, C.
700 1 $aRAFFO, M.A.
700 1 $aGERMAN, S.
700 1 $aPÉREZ, C.
700 1 $aGARMENDIA, G.
700 1 $aPAREJA, L.
700 1 $aRODRÍGUEZ, A.
700 1 $aPENDAS, C.
700 1 $aQUINCKE, M.
700 1 $aVÁZQUEZ, D.
700 1 $aRODRIGUEZ, M.
773 $tRevista INIA Uruguay, Diciembre 2023, no.75 p.54-58.
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