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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2016 |
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Actualizado : |
05/12/2016 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ROCHA, B. P.; REIS, M. O.; DRIEMEIER, D.; COOK, D.; CAMARGO, L. M.; RIET-CORREA, F.; EVENCIO-NETO, J; MENDONCA, F. |
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Afiliación : |
BRENA P. ROCHA, Universidad Federal de Pernambuco (UFPE); MATHEUS O. REIS, Universidad Federal de Rio Grande Do Sul (UFRGS); DAVID DRIEMEIER, Universidad Federal de Rio Grande Do Sul (UFRGS); DANIEL COOK, USDA-ARS (Agricultural Research Service); LÁZARO M. CAMARGO, Faculdade de Medicina Veterinária, Universidade de Cuiabá; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOAQUIM EVENCIO-NETO, Universidad Federal de Pernambuco (UFPE); FABIO S. MENDONCA, Universidade Federal l de Pernambuco (UFPE). |
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Título : |
Biópsia hepática como método diagnóstico para intoxicação por plantas que contém swainsonina. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Pesquisa Veterinaria Brasileira, 2016, v.36 (5): 373-377. |
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DOI : |
10.1590/S0100-736X2016000500003 |
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Idioma : |
Portugués |
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Notas : |
OPEN ACCESS |
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Contenido : |
ABSTRACT.-
With the aim to investigate the use of hepatic biopsies for the diagnosis of poisoning by swainsonine-containing plants, dry leaves of Ipomoea marcellia containing 0.02% of swainsonine were administered to goats. Group I, with six goats, ingested 4g/kg of dry plant (0.8mg of swainsonina/kg) daily until the observation of the first neurologic signs. Two goats that did not receive the plant were used as control (Group II). Hepatic biopsies with the Menghini needle were performed by the percutaneous technique at day zero and at weekly intervals after the start of the administration of I. marcellia. Biopsy samples were fixed in 10% formaline, processed routinely, and stained by hematoxilin-eosin and by lectins histochemistry. Hepatocellular vacuolization similar to those described in cases of lysosomal storage disease were identified in all goats of Group I from the seven day of plant consumption in the samples satained with hematoxylin-eosin. Using lectin histochemistry, consistent labellings were observed with Concanavalia ensiformis (Con-A) e Triticum vulgaris (WGA). It is concluded that routinely histological evaluation of liver biopsies can be used in the diagnosis of poisoning by swainsonine containing plants, even in goats without clinical signs, and lectin histochemistry which can be used as supplementary diagnostic method.
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RESUMO.
Neste trabalho objetivou-se avaliar a técnica de biópsia hepática como um teste de valor diagnóstico para intoxicações por plantas que contém swainsonina. Para isso, reproduziu-se experimentalmente a doença com as folhas secas de Ipomoea marcellia contendo 0,02% de swainsonina em caprinos. O Grupo I foi constituído por 6 caprinos que receberam a planta misturada a ração na dose de 4g/kg (0,8mg de swainsonina/kg) até a observação dos primeiros sinais clínicos neurológicos. Outros dois caprinos que não receberam a planta na dieta constituíram o grupo controle (Grupo II). Foram realizadas biópsias hepáticas pela técnica percutânea cega com agulha de Menghini, no dia zero e com intervalos semanais nos caprinos do experimento. As biópsias hepáticas foram fixadas em formol tamponado 10%, processadas rotineiramente, coradas pela hematoxilina-eosina e histoquímica de lectinas. Vacuolização hepatocelular similar àquelas descritas em caso de doença de depósito lisossomal foram identificadas em todos os caprinos do Grupo I no 7º dia de experimento nas amostras coradas pela hematoxilina-eosina. Em relação à histoquímica de lectinas, marcações consistentes foram obtidas com as lectinas Concanavalia ensiformis (Con-A) e Triticum vulgaris (WGA). Concluiu-se que a avaliação histológica rotineira de biópsias hepáticas pode ser usada no diagnóstico de intoxicações por plantas que contem swainsonina, mesmo em caprinos que não apresentam sinais clínicos, e que a histoquímica de lectinas pode ser usada como método diagnóstico complementar. MenosABSTRACT.-
With the aim to investigate the use of hepatic biopsies for the diagnosis of poisoning by swainsonine-containing plants, dry leaves of Ipomoea marcellia containing 0.02% of swainsonine were administered to goats. Group I, with six goats, ingested 4g/kg of dry plant (0.8mg of swainsonina/kg) daily until the observation of the first neurologic signs. Two goats that did not receive the plant were used as control (Group II). Hepatic biopsies with the Menghini needle were performed by the percutaneous technique at day zero and at weekly intervals after the start of the administration of I. marcellia. Biopsy samples were fixed in 10% formaline, processed routinely, and stained by hematoxilin-eosin and by lectins histochemistry. Hepatocellular vacuolization similar to those described in cases of lysosomal storage disease were identified in all goats of Group I from the seven day of plant consumption in the samples satained with hematoxylin-eosin. Using lectin histochemistry, consistent labellings were observed with Concanavalia ensiformis (Con-A) e Triticum vulgaris (WGA). It is concluded that routinely histological evaluation of liver biopsies can be used in the diagnosis of poisoning by swainsonine containing plants, even in goats without clinical signs, and lectin histochemistry which can be used as supplementary diagnostic method.
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RESUMO.
Neste trabalho objetivou-se avaliar a técnica de biópsia hepática como um teste de valor ... Presentar Todo |
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Palabras claves : |
LIVER BIOPSY; LYSOSOMAL STORAGE DISEASE; PLANT POISONING; POISONOUS PLANTS; SWAINSONINA. |
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Asunto categoría : |
-- |
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URL : |
http://130.211.64.33/pdf_artigos/26-05-2016_20-56Vet%202037%20pvb-4398%20LD.pdf
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Marc : |
LEADER 03859naa a2200289 a 4500
001 1056203
005 2016-12-05
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-736X2016000500003$2DOI
100 1 $aROCHA, B. P.
245 $aBiópsia hepática como método diagnóstico para intoxicação por plantas que contém swainsonina.$h[electronic resource]
260 $c2016
500 $aOPEN ACCESS
520 $aABSTRACT.- With the aim to investigate the use of hepatic biopsies for the diagnosis of poisoning by swainsonine-containing plants, dry leaves of Ipomoea marcellia containing 0.02% of swainsonine were administered to goats. Group I, with six goats, ingested 4g/kg of dry plant (0.8mg of swainsonina/kg) daily until the observation of the first neurologic signs. Two goats that did not receive the plant were used as control (Group II). Hepatic biopsies with the Menghini needle were performed by the percutaneous technique at day zero and at weekly intervals after the start of the administration of I. marcellia. Biopsy samples were fixed in 10% formaline, processed routinely, and stained by hematoxilin-eosin and by lectins histochemistry. Hepatocellular vacuolization similar to those described in cases of lysosomal storage disease were identified in all goats of Group I from the seven day of plant consumption in the samples satained with hematoxylin-eosin. Using lectin histochemistry, consistent labellings were observed with Concanavalia ensiformis (Con-A) e Triticum vulgaris (WGA). It is concluded that routinely histological evaluation of liver biopsies can be used in the diagnosis of poisoning by swainsonine containing plants, even in goats without clinical signs, and lectin histochemistry which can be used as supplementary diagnostic method. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMO. Neste trabalho objetivou-se avaliar a técnica de biópsia hepática como um teste de valor diagnóstico para intoxicações por plantas que contém swainsonina. Para isso, reproduziu-se experimentalmente a doença com as folhas secas de Ipomoea marcellia contendo 0,02% de swainsonina em caprinos. O Grupo I foi constituído por 6 caprinos que receberam a planta misturada a ração na dose de 4g/kg (0,8mg de swainsonina/kg) até a observação dos primeiros sinais clínicos neurológicos. Outros dois caprinos que não receberam a planta na dieta constituíram o grupo controle (Grupo II). Foram realizadas biópsias hepáticas pela técnica percutânea cega com agulha de Menghini, no dia zero e com intervalos semanais nos caprinos do experimento. As biópsias hepáticas foram fixadas em formol tamponado 10%, processadas rotineiramente, coradas pela hematoxilina-eosina e histoquímica de lectinas. Vacuolização hepatocelular similar àquelas descritas em caso de doença de depósito lisossomal foram identificadas em todos os caprinos do Grupo I no 7º dia de experimento nas amostras coradas pela hematoxilina-eosina. Em relação à histoquímica de lectinas, marcações consistentes foram obtidas com as lectinas Concanavalia ensiformis (Con-A) e Triticum vulgaris (WGA). Concluiu-se que a avaliação histológica rotineira de biópsias hepáticas pode ser usada no diagnóstico de intoxicações por plantas que contem swainsonina, mesmo em caprinos que não apresentam sinais clínicos, e que a histoquímica de lectinas pode ser usada como método diagnóstico complementar.
653 $aLIVER BIOPSY
653 $aLYSOSOMAL STORAGE DISEASE
653 $aPLANT POISONING
653 $aPOISONOUS PLANTS
653 $aSWAINSONINA
700 1 $aREIS, M. O.
700 1 $aDRIEMEIER, D.
700 1 $aCOOK, D.
700 1 $aCAMARGO, L. M.
700 1 $aRIET-CORREA, F.
700 1 $aEVENCIO-NETO, J
700 1 $aMENDONCA, F.
773 $tPesquisa Veterinaria Brasileira, 2016$gv.36 (5): 373-377.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
16/03/2020 |
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Actualizado : |
21/04/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MACHADO, D.N.; COSTA, E.C.; GUEDES, J.V.C.; BARBOSA, L.R.; MARTÍNEZ, G.; MAYORGA, S.I.; RAMOS, S.O.; BRANCO, M.; GARCÍA, A.; VANEGAS-RICO, J.M.; JIMÉNEZ-QUIROZ, E.; LAUDONIA, S.; NOVOSELSKY, T.; HODEL, D.R.; ARAKLIAN, G.; SILVA, H.; PERINI, C.R.; VALMORBIDA, I.; UGALDE, G.A.; ARNEMANN, J.A. |
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Afiliación : |
DAYANNA DO N. MACHADO, Doutoranda pelo Programa de Pós-Graduação em Engenharia Florestal, Universidade Federal de Santa Maria, Santa Maria, Brazil; Departamento de Defesa Fitossanitária, Santa Maria, Rio Grande do Sul, Brazil; ERVANDIL C. COSTA, Departamento de Defesa Fitossanitária, Santa Maria, Rio Grande do Sul, Brazil; JERSON V. C. GUEDES, Departamento de Defesa Fitossanitária, Santa Maria, Rio Grande do Sul, Brazil; LEONARDO R. BARBOSA, Empresa Brasileira de Pesquisa Agropecuária – Embrapa Florestas, Colombo, Paraná, Brazil; GONZALO ANIBAL MARTINEZ CROSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANDRA I. MAYORGA, Servicio Agrícola y Ganadero (SAG), Santiago, Chile; SERGIO O. RAMOS, Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Yuquerí, Concordia, Entre Ríos, Argentina; MANUELA BRANCO, Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal; ANDRÉ GARCIA, Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa, Portugal; JUAN MANUEL VANEGAS-RICO, Laboratorio de Control de Plagas, Unidad de Morfología y Función (UMF), Facultad de Estudios Superiores Iztacala, UNAM. Tlalnepantla de Baz, Mexico; EDUARDO JIMÉNEZ-QUIROZ, Laboratorio de Análisis y Referencia en Sanidad Forestal, Ciudad de México, Coyoacán, Mexico; STEFANIA LAUDONIA, Dipartimento di Agraria, Università degli Studi di Napoli Federico II, Portici, Italy; TANIA NOVOSELSKY, The Steinhardt Museum of Natural History, Israel National Center for Biodiversity Studies, Tel Aviv University, Tel Aviv, Israel; DONALD R. HODEL, University of California, Cooperative Extension, Alhambra, CA, United States; GEVORK ARAKELIAN, Entomologist, Los Angeles County Agricultural Commissioner, South Gate, CA, United States; HORACIO SILVA, Facultad de Agronomía Universidad de la República Uruguay, Paysandú, Uruguay; CLÉRISON R. PERINI, Departamento de Defesa Fitossanitária, Santa Maria, Rio Grande do Sul, Brazil; IVAIR VALMORBIDA, Department of Entomology, Iowa State University, Ames, IA, United States; GUSTAVO A. UGALDE, Departamento de Defesa Fitossanitária, Santa Maria, Rio Grande do Sul, Brazil; JONAS A. ARNEMANN, Departamento de Defesa Fitossanitária, Santa Maria, Rio Grande do Sul, Brazil. |
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Título : |
One maternal lineage leads the expansion of Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae) in the New and Old Worlds. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Scientific Reports, 1 December 2020, Volume 10, Issue 1, Article number 3487. OPEN ACCESS. Doi: https://doi.org/10.1038/s41598-020-60236-7 |
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ISSN : |
2045-2322 |
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DOI : |
10.1038/s41598-020-60236-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 11 July 2019 / Accepted 05 February 2020 / Published 26 February 2020.
Corresponding author: Machado, D.N. - email:dayanasmac@gmail.com |
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Contenido : |
ABSTRACT.
The bronze bug, Thaumastocoris peregrinus, an Australian native insect, has become a nearly worldwide invasive pest in the last 16 years and has been causing significant damage to eucalypts (Myrtaceae), including Eucalyptus spp. and Corymbia spp. Its rapid expansion leads to new questions about pathways and routes that T. peregrinus used to invade other continents and countries. We used mtDNA to characterize specimens of T. peregrinus collected from 10 countries where this species has become established, including six recently invaded countries: Chile, Israel, Mexico, Paraguay, Portugal, and the United States of America. We then combined our mtDNA data with previous data available from South Africa, Australia, and Europe to construct a world mtDNA network of haplotypes. Haplotype A was the most common present in all specimens of sites sampled in the New World, Europe, and Israel, however from Australia second more frequently. Haplotype D was the most common one from native populations in Australia. Haplotype A differs from the two major haplotypes found in South Africa (D and G), confirming that at least two independent invasions occurred, one from Australia to South Africa, and the other one from Australia to South America (A). In conclusion, Haplotype A has an invasion success over many countries in the World. Additionally, analyzing data from our work and previous reports, it is possible to suggest some invasive routes of T. peregrinus to predict such events and support preventive control measures. © 2020, The Author(s). MenosABSTRACT.
The bronze bug, Thaumastocoris peregrinus, an Australian native insect, has become a nearly worldwide invasive pest in the last 16 years and has been causing significant damage to eucalypts (Myrtaceae), including Eucalyptus spp. and Corymbia spp. Its rapid expansion leads to new questions about pathways and routes that T. peregrinus used to invade other continents and countries. We used mtDNA to characterize specimens of T. peregrinus collected from 10 countries where this species has become established, including six recently invaded countries: Chile, Israel, Mexico, Paraguay, Portugal, and the United States of America. We then combined our mtDNA data with previous data available from South Africa, Australia, and Europe to construct a world mtDNA network of haplotypes. Haplotype A was the most common present in all specimens of sites sampled in the New World, Europe, and Israel, however from Australia second more frequently. Haplotype D was the most common one from native populations in Australia. Haplotype A differs from the two major haplotypes found in South Africa (D and G), confirming that at least two independent invasions occurred, one from Australia to South Africa, and the other one from Australia to South America (A). In conclusion, Haplotype A has an invasion success over many countries in the World. Additionally, analyzing data from our work and previous reports, it is possible to suggest some invasive routes of T. peregrinus to predict such events and... Presentar Todo |
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Palabras claves : |
Thaumastocoris peregrinus. |
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Asunto categoría : |
K01 Ciencias forestales - Aspectos generales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14304/1/s41598-020-60236-7.pdf
https://www.nature.com/articles/s41598-020-60236-7.pdf
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Marc : |
LEADER 02932naa a2200397 a 4500
001 1060919
005 2020-04-21
008 2020 bl uuuu u00u1 u #d
022 $a2045-2322
024 7 $a10.1038/s41598-020-60236-7$2DOI
100 1 $aMACHADO, D.N.
245 $aOne maternal lineage leads the expansion of Thaumastocoris peregrinus (Hemiptera$bThaumastocoridae) in the New and Old Worlds.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 11 July 2019 / Accepted 05 February 2020 / Published 26 February 2020. Corresponding author: Machado, D.N. - email:dayanasmac@gmail.com
520 $aABSTRACT. The bronze bug, Thaumastocoris peregrinus, an Australian native insect, has become a nearly worldwide invasive pest in the last 16 years and has been causing significant damage to eucalypts (Myrtaceae), including Eucalyptus spp. and Corymbia spp. Its rapid expansion leads to new questions about pathways and routes that T. peregrinus used to invade other continents and countries. We used mtDNA to characterize specimens of T. peregrinus collected from 10 countries where this species has become established, including six recently invaded countries: Chile, Israel, Mexico, Paraguay, Portugal, and the United States of America. We then combined our mtDNA data with previous data available from South Africa, Australia, and Europe to construct a world mtDNA network of haplotypes. Haplotype A was the most common present in all specimens of sites sampled in the New World, Europe, and Israel, however from Australia second more frequently. Haplotype D was the most common one from native populations in Australia. Haplotype A differs from the two major haplotypes found in South Africa (D and G), confirming that at least two independent invasions occurred, one from Australia to South Africa, and the other one from Australia to South America (A). In conclusion, Haplotype A has an invasion success over many countries in the World. Additionally, analyzing data from our work and previous reports, it is possible to suggest some invasive routes of T. peregrinus to predict such events and support preventive control measures. © 2020, The Author(s).
653 $aThaumastocoris peregrinus
700 1 $aCOSTA, E.C.
700 1 $aGUEDES, J.V.C.
700 1 $aBARBOSA, L.R.
700 1 $aMARTÍNEZ, G.
700 1 $aMAYORGA, S.I.
700 1 $aRAMOS, S.O.
700 1 $aBRANCO, M.
700 1 $aGARCÍA, A.
700 1 $aVANEGAS-RICO, J.M.
700 1 $aJIMÉNEZ-QUIROZ, E.
700 1 $aLAUDONIA, S.
700 1 $aNOVOSELSKY, T.
700 1 $aHODEL, D.R.
700 1 $aARAKLIAN, G.
700 1 $aSILVA, H.
700 1 $aPERINI, C.R.
700 1 $aVALMORBIDA, I.
700 1 $aUGALDE, G.A.
700 1 $aARNEMANN, J.A.
773 $tScientific Reports, 1 December 2020, Volume 10, Issue 1, Article number 3487. OPEN ACCESS. Doi: https://doi.org/10.1038/s41598-020-60236-7
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