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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2016 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
VERDES, J. M.; MÁRQUEZ, M.; CALLIARI, A.; BATES, D.; MORAÑA, J. A.; GIMENO, E. J.; ODRIOZOLA, E.; GIANNITTI, F.; GUERRERO, F.; FIDALGO, L. E.; PUMAROLA, M. |
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Afiliación : |
JOSÉ MANUEL VERDES, Universidad de la República (UdelaR)/ Facultad de Veterinaria; MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MERCEDES MÁRQUEZ, Universidad de Santiago de Compostela (USC); ALDO CALLIARI, Universidad de la República (UdelaR)/ Facultad de Veterinaria; MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); DANIEL BATES, Universidad de la República (UdelaR)/ Facultad de Veterinaria; JOSÉ ANTONIO MORAÑA, Universidad de la República (UdelaR)/ Facultad de Veterinaria; EDUARDO JUAN GIMENO, Universidad Nacional de la Plata (UNLP); ERNESTO ODRIOZOLA, INTA (Instituto Nacional de Tecnología Agropecuaria)- Balcarce; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENTINA GUERRERO, Universidad de Santiago de Compostela (USC); LUIS EUSEBIO FIDALGO, Universidad de Santiago de Compostela (USC); MARTÍ PUMAROLA, Universitat Autònoma de Barcelona. |
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Título : |
A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle. (Full Scientific Report) |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, 2015, v.27 (3): 278-286. |
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DOI : |
10.1177/1040638715582048 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Published April 21, 2015. |
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Contenido : |
ABSTRACT.
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
© 2015 The Author(s). MenosABSTRACT.
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on th... Presentar Todo |
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Palabras claves : |
BOVINE; CEREBELLAR DEGENERATION; CYTOSKELETAL DYSFUNCTION; PURKINJE CELLS; SOLANUM BONARIENSE. |
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Thesagro : |
BOVINOS. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02961naa a2200337 a 4500
001 1056202
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638715582048$2DOI
100 1 $aVERDES, J. M.
245 $aA novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle. (Full Scientific Report)$h[electronic resource]
260 $c2015
500 $aArticle history: First Published April 21, 2015.
520 $aABSTRACT. Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death. © 2015 The Author(s).
650 $aBOVINOS
653 $aBOVINE
653 $aCEREBELLAR DEGENERATION
653 $aCYTOSKELETAL DYSFUNCTION
653 $aPURKINJE CELLS
653 $aSOLANUM BONARIENSE
700 1 $aMÁRQUEZ, M.
700 1 $aCALLIARI, A.
700 1 $aBATES, D.
700 1 $aMORAÑA, J. A.
700 1 $aGIMENO, E. J.
700 1 $aODRIOZOLA, E.
700 1 $aGIANNITTI, F.
700 1 $aGUERRERO, F.
700 1 $aFIDALGO, L. E.
700 1 $aPUMAROLA, M.
773 $tJournal of Veterinary Diagnostic Investigation, 2015$gv.27 (3): 278-286.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
03/09/2020 |
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Actualizado : |
03/09/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
ERRECART, P,M.; MARINO, M.A.; AGNUSDEI, M.G.; LATTANZI, F.; DURAND, J-L |
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Afiliación : |
PEDRO MANUEL ERRECART, Unidad Integrada Balcarce (Instituto Nacional de Tecnología Agropecuaria EEA Balcarce-Facultad de Ciencias Agrarias Universidad Nacional de Mar del Plata), Ruta Nacional 226 km 73.5, Balcarce, Argentina.; MARÍA ALEJANDRA MARINO, Unidad Integrada Balcarce (Instituto Nacional de Tecnología Agropecuaria EEA Balcarce-Facultad de Ciencias Agrarias Universidad Nacional de Mar del Plata), Ruta Nacional 226 km 73.5, Balcarce, Argentina.; MÓNICA GRACIELA AGNUSDEI, Unidad Integrada Balcarce (Instituto Nacional de Tecnología Agropecuaria EEA Balcarce-Facultad de Ciencias Agrarias Universidad Nacional de Mar del Plata), Ruta Nacional 226 km 73.5, Balcarce, Argentina.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JEAN-LOUIS DURAND, Unité de Recherche Pluridisciplinaire Prairies Plantes Fourragères (URP3F), INRA, Route de Saintes, Lusignan, France. |
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Título : |
The intensity of water stress dictates whether the N status of temperate-type perennial grass swards is affected by drought. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Field Crops Research, 15 October 2020, Volume 257, Article number 107928. Doi: https://doi.org/10.1016/j.fcr.2020.107928 |
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DOI : |
10.1016/j.fcr.2020.107928 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 February 2020/ Received in revised form 23 July 2020/ Accepted 27 July 2020. Corresponding author:
E-mail address: errecart.pedro@inta.gob.ar (P.M. Errecart). |
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Contenido : |
Abstract:Temperate-type, summer-active cool-season perennial grasses are frequently exposed to dry spells during the warm season due to the high atmospheric demand and fast soil desiccation. Since water availability is essential for sward nitrogen (N) uptake and most mineral N is located in the topsoil, water stress may hinder sward N nutrition if it reduces N supply. In hydro-halomorphic soils, imbalances between soil N supply and sward N demand during summer could be further aggravated by, first, the limited water holding capacity of the topsoil, and second, potential availability of water from deep, N-poor soil horizons that are kept moist by a shallow water table. The aim of this work was to quantify the effect of N and water availability on N status and productivity of temperate-type perennial swards growing in a hydro-halomorphic soil of the Flooding Pampa. During two consecutive years, shoot and root dynamics, as well as radiation capture, N nutrition index (NNI), soil water availability and leaf water potential were followed over six weeks long mid-summer regrowths of old stands (>9 years) of temperate-type tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] sown on a typical Natraqualf soil in Balcarce, Argentina. Swards were either rainfed or irrigated and fertilized with either 40 or 200 kg de N ha?1. Root systems were large (5.8?9.3 Mg DM roots ha?1), but concentrated mostly in the 0.2 m topsoil (66%?75% of root biomass or length). Despite invariably high available water below 0.4 m throughout both experimental periods, rainfed swards faced transient episodes of water stress whenever the upper soil layer dried out. Tall fescue swards were strongly co-limited by water and N during summer, showing comparable, very high responses to both N addition and irrigation. Further, forage yield showed synergistic increases when swards received both supplemental water and N simultaneously. This was mainly due to consistent synergistic responses in radiation use efficiency and leaf lifespan. Sward NNI was depressed only by an historically severe drought but was not altered when water stress intensity was lower, close to average for the season in the area. Therefore, the major causes of typically low summer productivity of temperate-type tall fescue growing in hydro-halomorphic soils of the Flooding Pampa are the intrinsic effects of low N and low water availability, with only uncommonly extreme water stress episodes reducing yield indirectly through an aggravation of crop N status. MenosAbstract:Temperate-type, summer-active cool-season perennial grasses are frequently exposed to dry spells during the warm season due to the high atmospheric demand and fast soil desiccation. Since water availability is essential for sward nitrogen (N) uptake and most mineral N is located in the topsoil, water stress may hinder sward N nutrition if it reduces N supply. In hydro-halomorphic soils, imbalances between soil N supply and sward N demand during summer could be further aggravated by, first, the limited water holding capacity of the topsoil, and second, potential availability of water from deep, N-poor soil horizons that are kept moist by a shallow water table. The aim of this work was to quantify the effect of N and water availability on N status and productivity of temperate-type perennial swards growing in a hydro-halomorphic soil of the Flooding Pampa. During two consecutive years, shoot and root dynamics, as well as radiation capture, N nutrition index (NNI), soil water availability and leaf water potential were followed over six weeks long mid-summer regrowths of old stands (>9 years) of temperate-type tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] sown on a typical Natraqualf soil in Balcarce, Argentina. Swards were either rainfed or irrigated and fertilized with either 40 or 200 kg de N ha?1. Root systems were large (5.8?9.3 Mg DM roots ha?1), but concentrated mostly in the 0.2 m topsoil (66%?75% of root biomass or length). Despite invariably high availa... Presentar Todo |
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Thesagro : |
ESTRES HIDRICO; NITROGEN. |
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NAL Tesauro : |
FORAGE YIELD; NITROGEN NUTRITION INDEX; RESOURCE CO-LIMITATION; ROOTS; WATER-NITROGEN INTERACTION. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03615naa a2200277 a 4500
001 1061293
005 2020-09-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.fcr.2020.107928$2DOI
100 1 $aERRECART, P,M.
245 $aThe intensity of water stress dictates whether the N status of temperate-type perennial grass swards is affected by drought.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 28 February 2020/ Received in revised form 23 July 2020/ Accepted 27 July 2020. Corresponding author: E-mail address: errecart.pedro@inta.gob.ar (P.M. Errecart).
520 $aAbstract:Temperate-type, summer-active cool-season perennial grasses are frequently exposed to dry spells during the warm season due to the high atmospheric demand and fast soil desiccation. Since water availability is essential for sward nitrogen (N) uptake and most mineral N is located in the topsoil, water stress may hinder sward N nutrition if it reduces N supply. In hydro-halomorphic soils, imbalances between soil N supply and sward N demand during summer could be further aggravated by, first, the limited water holding capacity of the topsoil, and second, potential availability of water from deep, N-poor soil horizons that are kept moist by a shallow water table. The aim of this work was to quantify the effect of N and water availability on N status and productivity of temperate-type perennial swards growing in a hydro-halomorphic soil of the Flooding Pampa. During two consecutive years, shoot and root dynamics, as well as radiation capture, N nutrition index (NNI), soil water availability and leaf water potential were followed over six weeks long mid-summer regrowths of old stands (>9 years) of temperate-type tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] sown on a typical Natraqualf soil in Balcarce, Argentina. Swards were either rainfed or irrigated and fertilized with either 40 or 200 kg de N ha?1. Root systems were large (5.8?9.3 Mg DM roots ha?1), but concentrated mostly in the 0.2 m topsoil (66%?75% of root biomass or length). Despite invariably high available water below 0.4 m throughout both experimental periods, rainfed swards faced transient episodes of water stress whenever the upper soil layer dried out. Tall fescue swards were strongly co-limited by water and N during summer, showing comparable, very high responses to both N addition and irrigation. Further, forage yield showed synergistic increases when swards received both supplemental water and N simultaneously. This was mainly due to consistent synergistic responses in radiation use efficiency and leaf lifespan. Sward NNI was depressed only by an historically severe drought but was not altered when water stress intensity was lower, close to average for the season in the area. Therefore, the major causes of typically low summer productivity of temperate-type tall fescue growing in hydro-halomorphic soils of the Flooding Pampa are the intrinsic effects of low N and low water availability, with only uncommonly extreme water stress episodes reducing yield indirectly through an aggravation of crop N status.
650 $aFORAGE YIELD
650 $aNITROGEN NUTRITION INDEX
650 $aRESOURCE CO-LIMITATION
650 $aROOTS
650 $aWATER-NITROGEN INTERACTION
650 $aESTRES HIDRICO
650 $aNITROGEN
700 1 $aMARINO, M.A.
700 1 $aAGNUSDEI, M.G.
700 1 $aLATTANZI, F.
700 1 $aDURAND, J-L
773 $tField Crops Research, 15 October 2020, Volume 257, Article number 107928. Doi: https://doi.org/10.1016/j.fcr.2020.107928
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