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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
21/02/2014 |
Actualizado : |
25/06/2019 |
Tipo de producción científica : |
Documentos |
Autor : |
PÉREZ GOMAR, E.; RISSO, D. |
Afiliación : |
ENRIQUE PEREZ GOMAR CAPURRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO FERNANDO RISSO RIET, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Riego de forrajeras en suelos de Basalto. |
Fecha de publicación : |
2001 |
Fuente / Imprenta : |
ln: INIA Tacuarembó; Unidad Experimental Glencoe. Producción animal y pasturas en Basalto. Día de campo, 15 noviembre, Paysandú, 2001. Tacuarembó (Uruguay): INIA, 2001. |
Páginas : |
p. 15 |
Serie : |
(INIA Serie Actividades de Difusión ; 271) |
Idioma : |
Español |
Palabras claves : |
ANIMAL PRODUCTION; PASTURE. |
Thesagro : |
PLANTAS FORRAJERAS; RIEGO; SUELO BASALTICO; URUGUAY. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9722/1/SAD-271p15.pdf
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Marc : |
LEADER 00703naa a2200217 a 4500 001 1025024 005 2019-06-25 008 2001 bl uuuu u00u1 u #d 100 1 $aPÉREZ GOMAR, E. 245 $aRiego de forrajeras en suelos de Basalto. 260 $c2001 300 $ap. 15 490 $a(INIA Serie Actividades de Difusión ; 271) 650 $aPLANTAS FORRAJERAS 650 $aRIEGO 650 $aSUELO BASALTICO 650 $aURUGUAY 653 $aANIMAL PRODUCTION 653 $aPASTURE 700 1 $aRISSO, D. 773 $tln: INIA Tacuarembó; Unidad Experimental Glencoe. Producción animal y pasturas en Basalto. Día de campo, 15 noviembre, Paysandú, 2001. Tacuarembó (Uruguay): INIA, 2001.
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Registro original : |
INIA Tacuarembó (TBO) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
24/10/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
B - 4 |
Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
ISSN : |
1948-5948 |
DOI : |
10.4172/1948-5948.1000155 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500 001 1051279 005 2019-10-15 008 2014 bl uuuu u00u1 u #d 022 $a1948-5948 024 7 $a10.4172/1948-5948.1000155$2DOI 100 1 $aALEM, D. 245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource] 260 $c2014 520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. 650 $aACTIVIDAD ANTIMICROBIANA 650 $aALTERNARIA 650 $aBIOTECNOLOGÍA 650 $aPÉPTIDOS 700 1 $aDIAZ, P. 700 1 $aLEONI, C. 700 1 $aDE SIMONE, S.G. 700 1 $aCORREA, A. 700 1 $aOPPEZZO, P. 700 1 $aDALLA RIZZA, M. 773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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