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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/03/2023 |
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Actualizado : |
27/04/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CORONA, M.; BRANCHICCELA, B.; ALBURAKI, M.; PALMER-YOUNG , E.C.; MADELLA , S.; CHEN, Y.; EVANS, J.D. |
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Afiliación : |
MIGUEL CORONA, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MOHAMED ALBURAKI, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; EVAN C. PALMER-YOUNG, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; SHAYNE MADELLA, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; YANPING CHEN, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States; JAY D. EVANS, Bee Research Laboratory, United States Department of Agriculture, Beltsville, MD, United States. |
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Título : |
Decoupling the effects of nutrition, age, and behavioral caste on honey bee physiology, immunity, and colony health. |
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Complemento del título : |
Original Research article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Frontiers in Physiology, 2023, Volume 14, article 1149840. OPEN ACCESS. doi: https://doi.org/10.3389/fphys.2023.1149840 |
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ISSN : |
1664-042X (Online) |
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DOI : |
10.3389/fphys.2023.1149840 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 January 2023; Accepted 22 February 2023; Published 13 March 2023. -- Correspondence author: Miguel Corona, Email:Miguel.Corona@usda.gov -- Edited by: Zhiguo Li, Fujian Agriculture and Forestry University, China. -- Reviewed by: Chunsheng Hou, Institute of Bast Fiber Crops (CAAS), China, Petar Hristov, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria. -- This article is part of the Research Topic Biotic and Abiotic Stresses on Honeybee Physiology and and Colony Health (https://www.frontiersin.org/research-topics/41726/biotic-and-abiotic-stresses-on-honeybee-physiology-and-colony-health#articles ). -- LICENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (http://creativecommons.org/licenses/by/4.0/ ) |
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Contenido : |
Nutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the experimental variables on viral titers, including higher Deformed wing virus (DWV) titers associated with foraging and age-related decline. In addition, nutrition significantly affected DWV titers in young nurses, with higher titers induced by pollen ingestion. In contrast, higher levels of Black queen cell virus (BQCV) were associated with pollen restriction. Finally, correlation, PCA, and NMDS analyses proved that behavior had had the strongest effect on gene expression and viral titers, followed by age and nutrition. These analyses also support multiple interactions among genes and virus analyzed, including negative correlations between the expression of genes encoding storage proteins associated with pollen ingestion and nursing (vg and mrjp1) with the expression of immune genes and DWV titers. Our results provide new insights into the proximal mechanisms by which nutritional stress is associated with changes in honey bee physiology, immunity, and viral titers. Copyright © 2023 Corona, Branchiccela, Alburaki, Palmer-Young, Madella, Chen and Evans. MenosNutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the ex... Presentar Todo |
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Palabras claves : |
Dorsal; DWV; Immune genes; Insulin; MFE; MRJP1; Vitellogenin. |
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Thesagro : |
APICULTURA; APIS MELLIFERA. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://www.frontiersin.org/articles/10.3389/fphys.2023.1149840/pdf
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Marc : |
LEADER 04394naa a2200337 a 4500
001 1063997
005 2023-04-27
008 2023 bl uuuu u00u1 u #d
022 $a1664-042X (Online)
024 7 $a10.3389/fphys.2023.1149840$2DOI
100 1 $aCORONA, M.
245 $aDecoupling the effects of nutrition, age, and behavioral caste on honey bee physiology, immunity, and colony health.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 23 January 2023; Accepted 22 February 2023; Published 13 March 2023. -- Correspondence author: Miguel Corona, Email:Miguel.Corona@usda.gov -- Edited by: Zhiguo Li, Fujian Agriculture and Forestry University, China. -- Reviewed by: Chunsheng Hou, Institute of Bast Fiber Crops (CAAS), China, Petar Hristov, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, Bulgaria. -- This article is part of the Research Topic Biotic and Abiotic Stresses on Honeybee Physiology and and Colony Health (https://www.frontiersin.org/research-topics/41726/biotic-and-abiotic-stresses-on-honeybee-physiology-and-colony-health#articles ). -- LICENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (http://creativecommons.org/licenses/by/4.0/ )
520 $aNutritional stress, especially a dearth of pollen, has been linked to honey bee colony losses. Colony-level experiments are critical for understanding the mechanisms by which nutritional stress affects individual honey bee physiology and pushes honey bee colonies to collapse. In this study, we investigated the impact of pollen restriction on key markers of honey bee physiology, main elements of the immune system, and predominant honey bee viruses. To achieve this objective, we uncoupled the effects of behavior, age, and nutritional conditions using a new colony establishment technique designed to control size, demography, and genetic background. Our results showed that the expression of storage proteins, including vitellogenin (vg) and royal jelly major protein 1 (mrjp1), were significantly associated with nursing, pollen ingestion, and older age. On the other hand, genes involved in hormonal regulation including insulin-like peptides (ilp1 and ilp2) and methyl farnesoate epoxidase (mfe), exhibited higher expression levels in young foragers from colonies not experiencing pollen restriction. In contrast, pollen restriction induced higher levels of insulin-like peptides in old nurses. On the other hand, we found a strong effect of behavior on the expression of all immune genes, with higher expression levels in foragers. In contrast, the effects of nutrition and age were significant only the expression of the regulatory gene dorsal. We also found multiple interactions of the experimental variables on viral titers, including higher Deformed wing virus (DWV) titers associated with foraging and age-related decline. In addition, nutrition significantly affected DWV titers in young nurses, with higher titers induced by pollen ingestion. In contrast, higher levels of Black queen cell virus (BQCV) were associated with pollen restriction. Finally, correlation, PCA, and NMDS analyses proved that behavior had had the strongest effect on gene expression and viral titers, followed by age and nutrition. These analyses also support multiple interactions among genes and virus analyzed, including negative correlations between the expression of genes encoding storage proteins associated with pollen ingestion and nursing (vg and mrjp1) with the expression of immune genes and DWV titers. Our results provide new insights into the proximal mechanisms by which nutritional stress is associated with changes in honey bee physiology, immunity, and viral titers. Copyright © 2023 Corona, Branchiccela, Alburaki, Palmer-Young, Madella, Chen and Evans.
650 $aAPICULTURA
650 $aAPIS MELLIFERA
653 $aDorsal
653 $aDWV
653 $aImmune genes
653 $aInsulin
653 $aMFE
653 $aMRJP1
653 $aVitellogenin
700 1 $aBRANCHICCELA, B.
700 1 $aALBURAKI, M.
700 1 $aPALMER-YOUNG , E.C.
700 1 $aMADELLA , S.
700 1 $aCHEN, Y.
700 1 $aEVANS, J.D.
773 $tFrontiers in Physiology, 2023, Volume 14, article 1149840. OPEN ACCESS. doi: https://doi.org/10.3389/fphys.2023.1149840
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
30/08/2022 |
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Actualizado : |
07/10/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MORALES-PIÑEYRUA, J.; DAMIÁN, J.P.; BANCHERO, G.; SANT`ANNA, A.C. |
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Afiliación : |
JESSICA TATIANA MORALES PIÑEYRUA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN P. DAMIÁN, Departamento de Biociencias Veterinaria, Facultad de Veterinaria, Universidad de la República. Alberto Lasplaces 1620, Montevideo, 11600, Uruguay.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALINE C SANT`ANNA, Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, 36036-900, Brazil. CNPq Researcher. |
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Título : |
The effects of heat stress on milk production and the grazing behavior of dairy Holstein cows milked by an automatic milking system. (Short Communication). |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Journal of Animal Science, june 2022, skac225. Doi: https://doi.org/10.1093/jas/skac225 |
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ISSN : |
1525-3163 |
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DOI : |
10.1093/jas/skac225. |
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Idioma : |
Inglés |
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Notas : |
Article history: Published 23 June 2022; Received 15 February 2022; Published 23 June 2022.
Corresponding author: Morales-Piñeyrúa, J.T.; Programa Nacional de Producción de Leche, Estación Experimental INIA La Estanzuela, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50 km 11, 70000, Colonia, Uruguay, email: jmorales@inia.org.uy -- |
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Contenido : |
Abstract:
The objective of the present study was to evaluate the productive performance and grazing behavior of 25 primiparous and 44 multiparous Holstein cows in a pasture-based automatic milking system (AMS) while experiencing heat stress (defined as a temperature-humidity index-THI ? 68). Productive traits were analyzed according to the THI from days 0, -1, -2, and -3 in relation to the milking day, and grazing behaviors (expressed as the % of daily observation time) were related to the average THI only on the day of observation. Milk yield was not associated with the THI on day 0, but a significant linear relationship was found with the THI on the three previous days, decreasing approximately 0.18 kg (primiparous) and 0.40 kg (multiparous) per THI unit increment. In contrast, for multiparous cows only, the milking frequency was positively associated with the THI on the day of evaluation but not on the previous days, increasing 0.01 milking/THI unit increments. Additionally, for each unit of THI increment, cows spent 0.14% more time standing, whereas they exhibited a decrease in grazing, lying, and ruminating behaviors time by 0.30%, 0.04%, and 0.70%, respectively, for both parities. In conclusion, milk loss was related to heat stress conditions from the previous days, but not milking frequency, which increased with the THI of the same milking day. Lower grazing, lying, and ruminating activities and greater standing behavior were observed due to heat stress. © The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. MenosAbstract:
The objective of the present study was to evaluate the productive performance and grazing behavior of 25 primiparous and 44 multiparous Holstein cows in a pasture-based automatic milking system (AMS) while experiencing heat stress (defined as a temperature-humidity index-THI ? 68). Productive traits were analyzed according to the THI from days 0, -1, -2, and -3 in relation to the milking day, and grazing behaviors (expressed as the % of daily observation time) were related to the average THI only on the day of observation. Milk yield was not associated with the THI on day 0, but a significant linear relationship was found with the THI on the three previous days, decreasing approximately 0.18 kg (primiparous) and 0.40 kg (multiparous) per THI unit increment. In contrast, for multiparous cows only, the milking frequency was positively associated with the THI on the day of evaluation but not on the previous days, increasing 0.01 milking/THI unit increments. Additionally, for each unit of THI increment, cows spent 0.14% more time standing, whereas they exhibited a decrease in grazing, lying, and ruminating behaviors time by 0.30%, 0.04%, and 0.70%, respectively, for both parities. In conclusion, milk loss was related to heat stress conditions from the previous days, but not milking frequency, which increased with the THI of the same milking day. Lower grazing, lying, and ruminating activities and greater standing behavior were observed due to heat stress. © The Author(... Presentar Todo |
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Palabras claves : |
Animal welfare; AUTOMATIC MILKING SYSTEM; DAIRY COW; HOLSTEIN; Milking visits; Pasture; Summer. |
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Thesagro : |
VACAS LECHERAS. |
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Asunto categoría : |
L02 Alimentación animal |
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Marc : |
LEADER 02878naa a2200289 a 4500
001 1063550
005 2022-10-07
008 2022 bl uuuu u00u1 u #d
022 $a1525-3163
024 7 $a10.1093/jas/skac225.$2DOI
100 1 $aMORALES-PIÑEYRUA, J.
245 $aThe effects of heat stress on milk production and the grazing behavior of dairy Holstein cows milked by an automatic milking system. (Short Communication).$h[electronic resource]
260 $c2022
500 $aArticle history: Published 23 June 2022; Received 15 February 2022; Published 23 June 2022. Corresponding author: Morales-Piñeyrúa, J.T.; Programa Nacional de Producción de Leche, Estación Experimental INIA La Estanzuela, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50 km 11, 70000, Colonia, Uruguay, email: jmorales@inia.org.uy --
520 $aAbstract: The objective of the present study was to evaluate the productive performance and grazing behavior of 25 primiparous and 44 multiparous Holstein cows in a pasture-based automatic milking system (AMS) while experiencing heat stress (defined as a temperature-humidity index-THI ? 68). Productive traits were analyzed according to the THI from days 0, -1, -2, and -3 in relation to the milking day, and grazing behaviors (expressed as the % of daily observation time) were related to the average THI only on the day of observation. Milk yield was not associated with the THI on day 0, but a significant linear relationship was found with the THI on the three previous days, decreasing approximately 0.18 kg (primiparous) and 0.40 kg (multiparous) per THI unit increment. In contrast, for multiparous cows only, the milking frequency was positively associated with the THI on the day of evaluation but not on the previous days, increasing 0.01 milking/THI unit increments. Additionally, for each unit of THI increment, cows spent 0.14% more time standing, whereas they exhibited a decrease in grazing, lying, and ruminating behaviors time by 0.30%, 0.04%, and 0.70%, respectively, for both parities. In conclusion, milk loss was related to heat stress conditions from the previous days, but not milking frequency, which increased with the THI of the same milking day. Lower grazing, lying, and ruminating activities and greater standing behavior were observed due to heat stress. © The Author(s) 2022. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.
650 $aVACAS LECHERAS
653 $aAnimal welfare
653 $aAUTOMATIC MILKING SYSTEM
653 $aDAIRY COW
653 $aHOLSTEIN
653 $aMilking visits
653 $aPasture
653 $aSummer
700 1 $aDAMIÁN, J.P.
700 1 $aBANCHERO, G.
700 1 $aSANT`ANNA, A.C.
773 $tJournal of Animal Science, june 2022, skac225. Doi: https://doi.org/10.1093/jas/skac225
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