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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
30/06/2021 |
Actualizado : |
30/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. |
Afiliación : |
ELEONOR CASTRO JANER, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; ANDRÉ DÍAZ, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA FONTES, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA BARAIBAR, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; TATIANA SAPORITI NOGUEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA ERNESTINA OLHAGARAY, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay. |
Título : |
Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 |
ISSN : |
1877-959X |
DOI : |
10.1016/j.ttbdis.2021.101747 |
Idioma : |
Inglés |
Notas : |
Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35).
Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com |
Contenido : |
ABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 MenosABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals an... Presentar Todo |
Palabras claves : |
Acaricides; Ixodidae; Reaxys retrieves substances; Rhipicephalus microplus. |
Asunto categoría : |
L73 Enfermedades de los animales |
Marc : |
LEADER 03773naa a2200265 a 4500 001 1062207 005 2021-06-30 008 2021 bl uuuu u00u1 u #d 022 $a1877-959X 024 7 $a10.1016/j.ttbdis.2021.101747$2DOI 100 1 $aCASTRO JANER, E. 245 $aMolecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.$h[electronic resource] 260 $c2021 500 $aArticle history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021. The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35). Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com 520 $aABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 653 $aAcaricides 653 $aIxodidae 653 $aReaxys retrieves substances 653 $aRhipicephalus microplus 700 1 $aDÍAZ, A. 700 1 $aFONTES, F. 700 1 $aBARAIBAR, F. 700 1 $aSAPORITI, T. 700 1 $aOLHAGARAY, M. E. 773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747
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Registros recuperados : 12 | |
1. | | CASTELLI, L.; BRANCHICCELA, B.; ZUNINO, P.; ANTÚNEZ, K. Efecto del glufosinato de amonio y sulfoxaflor en la microbiota intestinal, inmunidad y supervivencia de abejas melíferas. 131. (resúmen) Áreas temáticas: Microbiología. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.182-183. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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6. | | CASTELLI, L.; BALBUENA, S.; BRANCHICCELA, B.; ZUNINO, P.; LIBERTI, J.; ENGEL, P.; ANTÚNEZ, K. Impact of chronic exposure to sublethal doses of glyphosate on honey bee immunity, gut microbiota and infection by pathogens. Microorganisms, April 2021, Volume 9; Issue 4, Article number 845. OPEN ACCESS. https://doi.org/10.3390/microorganisms9040845 Article history: Received: 16 March 2021/Accepted: 31 March 2021/Published: 15 April 2021.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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7. | | MENDOZA, Y.; TOMASCO , I.H.; ANTÚNEZ, K.; CASTELLI, L.; BRANCHICCELA, B.; SANTOS, E.; INVERNIZZI, C. Unraveling honey bee-varroa destructor interaction: Multiple factors involved in differential resistance between two uruguayan populations. Veterinary Sciences, September 2020, Volume 7, Issue 3, Article number 116. OPEN ACCESS. Doi: https://doi.org/10.3390/vetsci7030116 Article history: Received: 26 July 2020/ Accepted: 17 August 2020/ Published: 20 August 2020./This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA), grant number PF10, and the Agencia Nacional de...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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8. | | BRANCHICCELA, B.; ANTÚNEZ, K.; ARREDONDO, D.; CASTELLI, L.; DIAZ, S.; JURI, P.; NOGUEIRA, E.; SILVA, C.; RAMALLO, G.; INVERNIZZI, C. Abejas tolerantes y susceptibles a Varroa destructor: consecuencias a nivel poblacional, sanitario y productivo. Sustentabilidad. Revista INIA Uruguay, Junio 2023, no.73, p.78-81. (Revista INIA; 73).Tipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA Las Brujas. |
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9. | | BRANCHICCELA, B.; CASTELLI, L.; DIAZ, S.; INVERNIZZI, C; MENDOZA, Y.; SANTOS, E.; SILVA, C.; ZUNINO, P.; ANTUNEZ, K, Can pollen supplementation mitigate the impact of nutritional stress on honey bee colonies? Journal of Apicultural Research, 2023, Volume 62, Issue 2, pp. 294-302. Doi: https://doi.org/10.1080/00218839.2021.1888537 Article history:Received 27 May 2020/ Accepted 08 Dec 2020/ Published online: 11 Mar 2021.Corresponding author. Email: bbranchiccela@inia.org.uy. This work was supported by the University of the Republic,Uruguay; under grant CSIC C624 and...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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10. | | ANTÚNEZ, K.; ARREDONDO, D.; CASTELLI, L.; SILVA, F.; AÑON, G.; HARRIET, J.; CAMPÁ, J.; INVERNIZZI, C.; ZUNINO, P. Desarrollo de una estrategia natural para el control de Varroa destructor, integrando el uso de probióticos y productos orgánicos. Montevideo (UY): INIA, 2019. 31 p. (Serie FPTA-INIA; 75). Proyecto FPTA-329: Desarrollo de una estrategia natural para el control de Varroa destructor, integrando el uso de probióticos y productos orgánicos. Periodo de ejecución: Febrero 2014 ? Junio 2017. Institución Ejecutora: MEC/ IIBCE...Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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11. | | INVERNIZZI, C.; ANTÚNEZ, K.; ARREDONDO, D.; BRANCHICCELA, B.; CASTELLI, L.; JURI, P.; MENDOZA, Y.; NOGUEIRA, E.; SALVARREY, S.; SANTOS, E. Situación sanitaria de las abejas melíferas en Uruguay: novedades de la última década. [Sanitary situation of honey bees in Uruguay: novelties of the last decade.]. Sección: Revisión. Veterinaria (Montev.), 2022, vol. 58, n. 217, e20225821704. https://doi.org/10.29155/VET.58.217.4 --- OPEN ACCESS. Article history: Recibido 05 Agosto 2021, Aceptado 23 Febrero 2022, Publicado 20 Mayo 2022. -- Autor para correspondencia: ciro@fcien.edu.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
Biblioteca(s): INIA Las Brujas. |
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12. | | BRANCHICCELA, B.; CASTELLI, L.; CORONA , M.; DIAZ-CETTI, S.; INVERNIZZI, C.; MARTÍNEZ DE LA ESCALERA, G; MENDOZA, Y.; SANTOS, E.; SILVA, C.; ZUNINO, P.; ANTÚNEZ, K. Impact of nutritional stress on the honeybee colony health. Scientific Reports, November 2019, volume 9, issue 1. OPEN ACCESS Article history: Received 28 January 2019/Accepted 20 June 2019/Published 12 July 2019.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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Registros recuperados : 12 | |
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