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1. | | ASTRUC, J.M.; BRITO, L.; BAPST, B.; BRUNI, G.; BURKE, M.; CIAPPESONI, G.; CONINGTON, J.; GARCÍA-BACCINO, C.; LARROQUE, H.; LEGARRA, A.; O'BRIEN, A.; POSTA, J.; ROBERT-GRANIÉ, C.; TEISSIER, M.; UGARTE, E.; MORENO-ROMIEUX, C.; BERRY, D. Selection tools to benefit from international cooperation in small ruminants: a comprehensive work package of the SMARTER project. [conference + oral presentation + video). In: Proceedings of the 44th ICAR Annual Conference virtually held from Leeuwarden, NL, 26-30 April 2021. "Circular farming and its impact on animal genetics, animal recording of data and cattle / herd management". Editors: G. de Jong, K. de Koning, H. van den Bijgaart, M. Burke and C. Mosconi. (ICAR Technical Serie s no. 25). Corresponding Author:Jean-Michel.Astruc@idele.frBiblioteca(s): INIA Las Brujas. |
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2. | | BRITO, L.F; BERRY, D.; LARROQUE, H.; SCHENKEI, F.S.; CIAPPESONI, G.; O’BRIEN, A.; TORTEREAU, F.; UGARTE, E.; PALHIERE, I.; BAPST, B.; JAKOBSEN, J.; ANTONAKOS, G.; KOMINAKIS, A.; CLEMENT, V.; BRUNI, G.; LOYWYCK, V.; MASSENDER, E.; OLIVEIRA, H.R.; POSTA, J.; ASTRUC, J.M. Genetic evaluation systems and breeding programs in sheep and goats: an international perspective. Annual Meeting of the European Federation of Animal Science, 17., No. 26, 2020. Virtual Meeting 1-4 December, 2020. DOI: DOI: 10.3920/978-90-8686-900-8. p. 560Biblioteca(s): INIA Tacuarembó. |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
30/09/2014 |
Actualizado : |
11/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
A - 2 |
Autor : |
ANTÚNEZ , K.; MENDOZA, Y.; SANTOS, E.; INVERNIZZI, C. |
Afiliación : |
YAMANDU MENDOZA SPINA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Differential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection. |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
Journal of Apicultural Research, v. 52, n. 5, p. 277-234, 2013. |
ISSN : |
0021-8839. |
DOI : |
10.3896/IBRA.1.52.5.09 |
Idioma : |
Inglés |
Notas : |
Article history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013. |
Contenido : |
Summary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of
honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae.
Resumen:
Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una
enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas
ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N.
ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas
fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del
traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y
menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron
artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina,
mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa
deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de
vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon
abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su
expresión diferencial podría estar asociada a la resistencia a N. ceranae. MenosSummary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a cen... Presentar Todo |
Palabras claves : |
NOSEMA CERANAE; NOSEMOSIS; PCR EN TIEMPO REAL; RESPUESTA INMUNE; VITELOGENINA. |
Thesagro : |
APICULTURA; APIS MELLIFERA; ENFERMEDADES DE LAS ABEJAS. |
Asunto categoría : |
L73 Enfermedades de los animales |
Marc : |
LEADER 04399naa a2200289 a 4500 001 1050723 005 2019-10-11 008 2013 bl uuuu u00u1 u #d 022 $a0021-8839. 024 7 $a10.3896/IBRA.1.52.5.09$2DOI 100 1 $aANTÚNEZ , K. 245 $aDifferential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection.$h[electronic resource] 260 $c2013 500 $aArticle history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013. 520 $aSummary: Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen. The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin, hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae. Resumen: Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N. ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina, mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su expresión diferencial podría estar asociada a la resistencia a N. ceranae. 650 $aAPICULTURA 650 $aAPIS MELLIFERA 650 $aENFERMEDADES DE LAS ABEJAS 653 $aNOSEMA CERANAE 653 $aNOSEMOSIS 653 $aPCR EN TIEMPO REAL 653 $aRESPUESTA INMUNE 653 $aVITELOGENINA 700 1 $aMENDOZA, Y. 700 1 $aSANTOS, E. 700 1 $aINVERNIZZI, C. 773 $tJournal of Apicultural Research$gv. 52, n. 5, p. 277-234, 2013.
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