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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/03/2021 |
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Actualizado : |
25/03/2021 |
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Tipo de producción científica : |
Artículos Indexados |
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Autor : |
NASCIMENTO, D. C.; DINI, M.; SAMPAIO, N. V.; SCHUCH, M. W. |
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Afiliación : |
DANIELE CAMARGO NASCIMENTO, Universidade Federal do Pampa/UNIPAMPA, Dom Pedrito, RS, Brasil; MAXIMILIANO ANTONIO DINI VIÑOLY, Universidade Federal de Pelotas/UFPel, Capão do Leão, RS, Brasil; NORTON VICTOR SAMPAIO, Universidade Federal do Pampa/UNIPAMPA, Dom Pedrito, RS, Brasil; MÁRCIA WULFF SCHUCH, Universidade Federal de Pelotas/UFPel, Capão do Leão, RS, Brasil. |
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Título : |
In vitro multiplication and rooting of grapevine; culture media and plant growth regulators. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Plant Cell Culture & Micropropagation, 2019, volume 15, n. 1, p. 1-7. OPEN ACCESS. Doi: https://doi.org/10.46526/pccm.2019.v15i1.136 |
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ISSN : |
1808-9909 |
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DOI : |
10.46526/pccm.2019.v15i1.136 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received in July, 2019 and approved in April, 2020. |
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Contenido : |
ABSTRACT.
Micropropagation is a technique that allows to obtain quality plants, making it an important tool in the production of grapevine matrices. Composition of the culture media and plant growth regulators are commonly tested to adapt micropropagation protocols. The objective of this study was to test concentrations of Murashige & Skoog (MS) culture media and BAP (6-benzylaminopurine) and IBA (indolbutyric acid) plant growth regulators in the multiplication and in vitro rooting stages as part of the elaboration of a Chardonnay grapevine micropropagation protocol. In both experiments, multiplication and rooting, the experimental design was completely randomized, with a bifactorial scheme, testing culture media (MS and MS/2) and BAP concentrations (0; 5; 10 μM) in the multiplication and of IBA (0; 0.5; 1.0; 1.5 μM) in rooting. For multiplication, the best results for number of buds, leaves and shoots were obtained with 10 μM BAP, and the concentrations of the culture media had no significant effect for most of the variables. For rooting, the MS media provided better results. In relation to IBA, the number and average root length were influenced by the concentration used, with 0.5 and 1.0 μM providing the best results. Grapevine explants may be multiplied in MS/2 media supplemented with 10 μM BAP and subsequently rooted in MS media with 0.5 μM IBA. |
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Palabras claves : |
Auxin; Cytokinin; MICROPROPAGATION; TISSUE CULTURE. |
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Thesagro : |
VITIS VINIFERA. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://pccm.ufla.br/index.php/plantcellculturemicropropagation/article/view/136
http://www.ainfo.inia.uy/digital/bitstream/item/15384/1/Nascimento-et-al.-2019.-In-vitro-multiplication-Plant-Cell.pdf
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Marc : |
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008 2019 bl uuuu u00u1 u #d
022 $a1808-9909
024 7 $a10.46526/pccm.2019.v15i1.136$2DOI
100 1 $aNASCIMENTO, D. C.
245 $aIn vitro multiplication and rooting of grapevine; culture media and plant growth regulators.$h[electronic resource]
260 $c2019
500 $aArticle history: Received in July, 2019 and approved in April, 2020.
520 $aABSTRACT. Micropropagation is a technique that allows to obtain quality plants, making it an important tool in the production of grapevine matrices. Composition of the culture media and plant growth regulators are commonly tested to adapt micropropagation protocols. The objective of this study was to test concentrations of Murashige & Skoog (MS) culture media and BAP (6-benzylaminopurine) and IBA (indolbutyric acid) plant growth regulators in the multiplication and in vitro rooting stages as part of the elaboration of a Chardonnay grapevine micropropagation protocol. In both experiments, multiplication and rooting, the experimental design was completely randomized, with a bifactorial scheme, testing culture media (MS and MS/2) and BAP concentrations (0; 5; 10 μM) in the multiplication and of IBA (0; 0.5; 1.0; 1.5 μM) in rooting. For multiplication, the best results for number of buds, leaves and shoots were obtained with 10 μM BAP, and the concentrations of the culture media had no significant effect for most of the variables. For rooting, the MS media provided better results. In relation to IBA, the number and average root length were influenced by the concentration used, with 0.5 and 1.0 μM providing the best results. Grapevine explants may be multiplied in MS/2 media supplemented with 10 μM BAP and subsequently rooted in MS media with 0.5 μM IBA.
650 $aVITIS VINIFERA
653 $aAuxin
653 $aCytokinin
653 $aMICROPROPAGATION
653 $aTISSUE CULTURE
700 1 $aDINI, M.
700 1 $aSAMPAIO, N. V.
700 1 $aSCHUCH, M. W.
773 $tPlant Cell Culture & Micropropagation, 2019, volume 15$gn. 1, p. 1-7. OPEN ACCESS. Doi: https://doi.org/10.46526/pccm.2019.v15i1.136
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 3 | |
| 1. |  | NASCIMENTO, D. C.; DINI, M.; CARPENEDO, S.; RASEIRA, M. DO C. B. Germination and development of 'Precocinho' peach embryos: asepsis and use of PPM tm in culture medium. Journal of Experimental Agriculture International, 2018, v. 22, n. 6, p. 1-7. Article no.JEAI.41065. Doi: https://doi.org/10.9734/JEAI/2018/41065 Article history: Received 19 February 2018; Accepted 27 April 2018; Published 2 May 2018.
Article information: Editor(s): (1) Biljana Bojovic, Assistant Professor, Faculty of Science, Institute of Biology and Ecology, University of...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 2. | | NASCIMENTO, D. C.; DINI, M.; MOREIRA, R. M.; SAMPAIO, N. V.; SCHUCH, M. W. Minicutting of chardonnay grapevine and SO4 grapevine rootstock. Revista Científica Rural, Bagé-RS, 2019, volume 21, no 3, p. 270-281. OPEN ACCESS. DOI: https://doi.org/10.30945/rcr-v21i3.3050 Article history: Submitted June 20, 2019; Accepted November 14, 2019.| Tipo: Artículos Indexados |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | NASCIMENTO, D. C.; DINI, M.; SAMPAIO, N. V.; SCHUCH, M. W. In vitro multiplication and rooting of grapevine; culture media and plant growth regulators. Plant Cell Culture & Micropropagation, 2019, volume 15, n. 1, p. 1-7. OPEN ACCESS. Doi: https://doi.org/10.46526/pccm.2019.v15i1.136 Article history: Received in July, 2019 and approved in April, 2020.| Tipo: Artículos Indexados |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 3 | |
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