| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha : |
03/12/2020 |
|
Actualizado : |
03/12/2020 |
|
Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
|
Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
|
Título : |
Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). |
|
Fecha de publicación : |
2020 |
|
Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
|
DOI : |
10.1007/s11240-020-01891-6 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
|
Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
|
Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
|
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
|
Marc : |
LEADER 03007naa a2200301 a 4500
001 1061539
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11240-020-01891-6$2DOI
100 1 $aNARANCIO, R.
245 $aCorrection to$bApplication of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6).$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020.
520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V.
653 $aAgrobacterium-mediated transformation
653 $aDelayed leaf senescence
653 $aMultigene
653 $aT-DNA
700 1 $aDING, Y.-L.
700 1 $aLIN, Y.-H.
700 1 $aSAHAB, S.
700 1 $aPANTER, S.
700 1 $aHAYES, M.
700 1 $aJOHN, U.
700 1 $aANDERSON, H.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
No copia registrada de este documento.
|
Volver
|
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha actual : |
29/04/2015 |
|
Actualizado : |
06/05/2015 |
|
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
|
Autor : |
CIAPPESONI, G.; SAN JULIAN, R.; NAVAJAS, E.; GIMENO, D.; GUTIERREZ-ZAMIT, E.; GOLDBERG, V.; BRITO, G. |
|
Afiliación : |
CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO SAN JULIAN SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO GIMENO, SUL (Secretariado Uruguayo de la Lana).; LUIS EMILIO GUTIERREZ ZAMIT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA GOLDBERG BIANCHI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Genetic evaluation of the Texel breed in Uruguay: I. Carcass quality traits. |
|
Fecha de publicación : |
2014 |
|
Fuente / Imprenta : |
In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY (ICoMST), 60., 2014, Punta del Este, Uruguay. Proceedings... Montevideo: INIA, 2014. |
|
Páginas : |
4 p. |
|
Idioma : |
Inglés |
|
Contenido : |
ABSTRACT.
Due to the relevance of carcass and meat quality traits as breeding objectives of Texel as sire terminal breed, a progeny testing was implemented. In general, these difficult to measure traits have received less attention despite their economic relevance, particularly in more extensive environments in which sheep production is mainly located. Carcass traits were recorded on 424 female and male lambs: Hot Carcass Weight (HCW), most valuable cuts (French Rack, Shoulder and Leg weights), morphometrics traits (Carcass and Leg length, Leg Circumference) and GR (indicator of carcass fatness). Traits as Scanning weight, Rib Eye Area and Fat Thickness are routinely in vivo recorded in the genetic evaluation system and genetic parameters were estimated with 3.109 records by a multivariate analysis. Estimated Progeny Difference (EPD) for in vivo and post-mortem carcass traits was performed. Heritability estimates were of moderate to high magnitude, in agreement with other studies. Although results confirm that there is a scope for genetic improvement, these preliminary values should be interpreted with caution because of the low number of animals. All correlations between EPD were positive and favourable except for fatness traits that depend heavily on factors such as the production system and the target market. |
|
Thesagro : |
CALIDAD DE CARNE; GANADO DE CARNE; TEXEL. |
|
Asunto categoría : |
L01 Ganadería |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/4409/1/Ciappesoni.-1-canal-Texel-ICoMST-2014.pdf
|
|
Marc : |
LEADER 02063nam a2200229 a 4500
001 1052588
005 2015-05-06
008 2014 bl uuuu u01u1 u #d
100 1 $aCIAPPESONI, G.
245 $aGenetic evaluation of the Texel breed in Uruguay$bI. Carcass quality traits.$h[electronic resource]
260 $aIn: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY (ICoMST), 60., 2014, Punta del Este, Uruguay. Proceedings... Montevideo: INIA$c2014
300 $a4 p.
520 $aABSTRACT. Due to the relevance of carcass and meat quality traits as breeding objectives of Texel as sire terminal breed, a progeny testing was implemented. In general, these difficult to measure traits have received less attention despite their economic relevance, particularly in more extensive environments in which sheep production is mainly located. Carcass traits were recorded on 424 female and male lambs: Hot Carcass Weight (HCW), most valuable cuts (French Rack, Shoulder and Leg weights), morphometrics traits (Carcass and Leg length, Leg Circumference) and GR (indicator of carcass fatness). Traits as Scanning weight, Rib Eye Area and Fat Thickness are routinely in vivo recorded in the genetic evaluation system and genetic parameters were estimated with 3.109 records by a multivariate analysis. Estimated Progeny Difference (EPD) for in vivo and post-mortem carcass traits was performed. Heritability estimates were of moderate to high magnitude, in agreement with other studies. Although results confirm that there is a scope for genetic improvement, these preliminary values should be interpreted with caution because of the low number of animals. All correlations between EPD were positive and favourable except for fatness traits that depend heavily on factors such as the production system and the target market.
650 $aCALIDAD DE CARNE
650 $aGANADO DE CARNE
650 $aTEXEL
700 1 $aSAN JULIAN, R.
700 1 $aNAVAJAS, E.
700 1 $aGIMENO, D.
700 1 $aGUTIERREZ-ZAMIT, E.
700 1 $aGOLDBERG, V.
700 1 $aBRITO, G.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
