| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha : |
18/12/2020 |
|
Actualizado : |
18/12/2020 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
DORSCH, M.; REGIDOR-CERRILLO, J.; ORTEGA-MORA, L.M.; SCIOLI, M.V.; MORRELL, E.L.; CANTÓN, G.J.; MOORE, D.P.; HECKER, Y.P. |
|
Afiliación : |
MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay;Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina; JAVIER REGIDOR-CERRILLO, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; LUIS M. ORTEGA-MORA, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; MARÍA V. SCIOLI, María V. Scioli National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; ELEONORA L. MORRELL, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; GERMÁN J. CANTÓN, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; DADÍN P. MOORE, Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina.; YANINA P. HECKER, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina. |
|
Título : |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina. |
|
Fecha de publicación : |
2020 |
|
Fuente / Imprenta : |
Parasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x |
|
DOI : |
10.1007/s00436-020-06922-x |
|
Idioma : |
Inglés |
|
Notas : |
Article history:Received: 12 August 2020 /Accepted: 5 October 2020 |
|
Contenido : |
Abstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. |
|
Palabras claves : |
ABORTION; BOVINE; MICROSATELLITE MARKERS; NEOSPORA CANINUM; PLATAFORMA SALUD ANIMAL. |
|
Thesagro : |
ARGENTINA. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 02492naa a2200301 a 4500
001 1061592
005 2020-12-18
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s00436-020-06922-x$2DOI
100 1 $aDORSCH, M.
245 $aMicrosatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 12 August 2020 /Accepted: 5 October 2020
520 $aAbstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.
650 $aARGENTINA
653 $aABORTION
653 $aBOVINE
653 $aMICROSATELLITE MARKERS
653 $aNEOSPORA CANINUM
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aREGIDOR-CERRILLO, J.
700 1 $aORTEGA-MORA, L.M.
700 1 $aSCIOLI, M.V.
700 1 $aMORRELL, E.L.
700 1 $aCANTÓN, G.J.
700 1 $aMOORE, D.P.
700 1 $aHECKER, Y.P.
773 $tParasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
15/04/2021 |
|
Actualizado : |
02/09/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
CASTELLI, L.; BALBUENA, S.; BRANCHICCELA, B.; ZUNINO, P.; LIBERTI, J.; ENGEL, P.; ANTÚNEZ, K. |
|
Afiliación : |
LORELEY CASTELLI, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE).; SOFÍA BALBUENA, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE).; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE).; JOANITO LIBERTI, Department of Fundamental Microbiology, University of Lausanne.; PHILIPP ENGEL, Department of Fundamental Microbiology, University of Lausanne.; KARINA ANTÚNEZ, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE). |
|
Título : |
Impact of chronic exposure to sublethal doses of glyphosate on honey bee immunity, gut microbiota and infection by pathogens. |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
Microorganisms, April 2021, Volume 9; Issue 4, Article number 845. OPEN ACCESS. https://doi.org/10.3390/microorganisms9040845 |
|
DOI : |
10.3390/microorganisms9040845 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 16 March 2021/Accepted: 31 March 2021/Published: 15 April 2021. |
|
Contenido : |
Abstract: Glyphosate is the most used pesticide around the world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts on behavior, cognitive, sensory and developmental abilities, its use continues to grow. Recent studies have shown that it also alters the composition of the honey bee gut microbiota. In this study we explored the impact of chronic exposure to sublethal doses of glyphosate on the honey bee gut microbiota and its effects on the immune response, infection by Nosema ceranae and Deformed wing virus (DWV) and honey bee survival. Glyphosate combined with N. ceranae infection altered the structure and composition of the honey bee gut microbiota, for example by decreasing the relative abundance of the core members Snodgrassella alvi and Lactobacillus apis. Glyphosate increased the expression of some immune genes, possibly representing a physiological response to mitigate its negative effects. However, this response was not sufficient to maintain honey bee health, as glyphosate promoted the replication of DWV and decreased the expression of vitellogenin, which were accompanied by a reduced life span. Infection by N. ceranae also alters honey bee immunity although no synergistic effect with glyphosate was observed. These results corroborate previous findings suggesting deleterious effects of widespread use of glyphosate on honey bee health, and they contribute to elucidate the physiological mechanisms underlying a global decline of pollination services. MenosAbstract: Glyphosate is the most used pesticide around the world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts on behavior, cognitive, sensory and developmental abilities, its use continues to grow. Recent studies have shown that it also alters the composition of the honey bee gut microbiota. In this study we explored the impact of chronic exposure to sublethal doses of glyphosate on the honey bee gut microbiota and its effects on the immune response, infection by Nosema ceranae and Deformed wing virus (DWV) and honey bee survival. Glyphosate combined with N. ceranae infection altered the structure and composition of the honey bee gut microbiota, for example by decreasing the relative abundance of the core members Snodgrassella alvi and Lactobacillus apis. Glyphosate increased the expression of some immune genes, possibly representing a physiological response to mitigate its negative effects. However, this response was not sufficient to maintain honey bee health, as glyphosate promoted the replication of DWV and decreased the expression of vitellogenin, which were accompanied by a reduced life span. Infection by N. ceranae also alters honey bee immunity although no synergistic effect with glyphosate was observed. These results corroborate previous findings suggesting deleterious effects of widespread use of glyphosate on honey bee health, and they contribute to elucidate the physiological mechanisms underlying a g... Presentar Todo |
|
Palabras claves : |
DEFORMED WING VIRUS; GLYPHOSATE; HONEY BEE GUT MICROBIOTA; HONEY BEE HEALTH; HONEY BEE IMMUNE RESPONSE; NOSEMA CERANAE; PESTICIDES. |
|
Thesagro : |
ABEJAS; APICULTURA. |
|
Asunto categoría : |
-- |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16668/1/microorganisms-09-00845-v2.pdf
https://www.mdpi.com/2076-2607/9/4/845
|
|
Marc : |
LEADER 02651naa a2200325 a 4500
001 1061984
005 2022-09-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/microorganisms9040845$2DOI
100 1 $aCASTELLI, L.
245 $aImpact of chronic exposure to sublethal doses of glyphosate on honey bee immunity, gut microbiota and infection by pathogens.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 16 March 2021/Accepted: 31 March 2021/Published: 15 April 2021.
520 $aAbstract: Glyphosate is the most used pesticide around the world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts on behavior, cognitive, sensory and developmental abilities, its use continues to grow. Recent studies have shown that it also alters the composition of the honey bee gut microbiota. In this study we explored the impact of chronic exposure to sublethal doses of glyphosate on the honey bee gut microbiota and its effects on the immune response, infection by Nosema ceranae and Deformed wing virus (DWV) and honey bee survival. Glyphosate combined with N. ceranae infection altered the structure and composition of the honey bee gut microbiota, for example by decreasing the relative abundance of the core members Snodgrassella alvi and Lactobacillus apis. Glyphosate increased the expression of some immune genes, possibly representing a physiological response to mitigate its negative effects. However, this response was not sufficient to maintain honey bee health, as glyphosate promoted the replication of DWV and decreased the expression of vitellogenin, which were accompanied by a reduced life span. Infection by N. ceranae also alters honey bee immunity although no synergistic effect with glyphosate was observed. These results corroborate previous findings suggesting deleterious effects of widespread use of glyphosate on honey bee health, and they contribute to elucidate the physiological mechanisms underlying a global decline of pollination services.
650 $aABEJAS
650 $aAPICULTURA
653 $aDEFORMED WING VIRUS
653 $aGLYPHOSATE
653 $aHONEY BEE GUT MICROBIOTA
653 $aHONEY BEE HEALTH
653 $aHONEY BEE IMMUNE RESPONSE
653 $aNOSEMA CERANAE
653 $aPESTICIDES
700 1 $aBALBUENA, S.
700 1 $aBRANCHICCELA, B.
700 1 $aZUNINO, P.
700 1 $aLIBERTI, J.
700 1 $aENGEL, P.
700 1 $aANTÚNEZ, K.
773 $tMicroorganisms, April 2021, Volume 9; Issue 4, Article number 845. OPEN ACCESS. https://doi.org/10.3390/microorganisms9040845
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
