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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó. |
Fecha : |
01/12/2020 |
Actualizado : |
21/05/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PARODI, P.; CORBELLINI, L.G.; LEOTTI, V.B.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M.; ARMÚA-FERNÁNDEZ, M.T. |
Afiliación : |
PABLO ANDRÉS PARODI TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS G. CORBELLINI, Laboratório de Epidemiología Veterinária, Facultad de Veterinária, Departamento de Medicina Veterinária Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; VANESSA B. LEOTTI, Departamento de Estatística, Instituto de Matemática e Estatística, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; RODOLFO RIVERO, Laboratorio Regional Noroeste “Miguel C. Rubino”, División de Laboratorios Veterinarios “Miguel C. Rubino”, Paysandú, Uruguay; MÓNICA CECILIA MIRABALLES FERRER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ M. VENZAL, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay; MARÍA T. ARMÚA-FERNÁNDEZ, Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay. |
Título : |
Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report) |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742 |
DOI : |
10.1177/1040638720975742 |
Idioma : |
Inglés |
Notas : |
Article history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com |
Contenido : |
Abstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent. |
Palabras claves : |
ANAPLASMA MARGINALE; BABESIA SPP; CATTLE; ENFERMEDADES DE LOS ANIMALES; MULTIPLEX PCR; PLATAFORMA DE SALUD ANIMAL; SENSITIVITY; SPECIFICITY; TICK-BORNE DISEASES. |
Asunto categoría : |
E16 Enfermedades de los animales |
Marc : |
LEADER 02587naa a2200337 a 4500 001 1061695 005 2021-05-21 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1177/1040638720975742$2DOI 100 1 $aPARODI, P. 245 $aValidation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report)$h[electronic resource] 260 $c2021 500 $aArticle history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples. Corresponding author: Pablo Parodi: pabloparodi7@gmail.com 520 $aAbstract: Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent. 653 $aANAPLASMA MARGINALE 653 $aBABESIA SPP 653 $aCATTLE 653 $aENFERMEDADES DE LOS ANIMALES 653 $aMULTIPLEX PCR 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aSENSITIVITY 653 $aSPECIFICITY 653 $aTICK-BORNE DISEASES 700 1 $aCORBELLINI, L.G. 700 1 $aLEOTTI, V.B. 700 1 $aRIVERO, R. 700 1 $aMIRABALLES, C. 700 1 $aRIET-CORREA, F. 700 1 $aVENZAL, J.M. 700 1 $aARMÚA-FERNÁNDEZ, M.T. 773 $tJournal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742
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Registro original : |
INIA La Estanzuela (LE) |
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Registros recuperados : 30 | |
24. | | PARODI, P.; ARMÚA-FERNÁNDEZ, M.T.; CORBELLINI, L.G.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M. Description of bovine babesiosis and anaplasmosis outbreaks in northern Uruguay between 2016 and 2018. Veterinary Parasitology: Regional Studies and Reports, 2022, Volume 29, Article number 100700. doi: https://doi.org/10.1016/j.vprsr.2022.100700 Article history: Received 7 April 2021; Received in revised form 27 November 2021; Accepted 26 January 2022; Available online 2 February 2022.
Corresponding author: Parodi, P.; Instituto Nacional de Investigación Agropecuaria,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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25. | | LUCAS, M.; KROLOW, T.K.; RIET-CORREA, F.; BARROS, A.T.M.; KRÜGER, R.F.; SARAVIA, A.; MIRABALLES, C. Diversity and seasonality of horse flies (Diptera: Tabanidae) in Uruguay. Scientific Reports, 10, no. 401, 2020. OPEN ACCESS. Article history: Received24 October 2019 // Accepted29 December 2019 // Published15 January 2020. Corresponding author: cmiraballes@inia.org.uy // Acknowledgements: We would like to acknowledge Marcelo Alfonso for the help provided with...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Tacuarembó. |
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26. | | MIRABALLES, C.; SÁNCHEZ, J.; BARROS, A.T.M.; HITATEGUY, S.; MORENO, P.; SAPORITI, T.; RIET-CORREA, F. Influence of selective treatment of bulls on the infestation of Haematobia irritans on untreated cows. Veterinary Parasitology, 2018, v. 260, p. 58–62. Article history: Received 25 May 2018, Revised 23 August 2018, Accepted 24 August 2018, Available online 27 August 2018.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Tacuarembó. |
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27. | | MIRABALLES, C.; BARROS, A.T.M.; LUCAS, M.; KLAFKE, G.M.; DOMINGUES, L.N.; RIET-CORREA, F. Susceptibility of field populations of Haematobia irritans to fipronil in Uruguay. Pesquisa Veterinaria Brasileira, 2021, Volume 41, Article number e06821. OPEN ACCESS. Doi: https://doi.org/10.1590/1678-5150-PVB-6821 Article history: 1 Received on December 4, 2020. Accepted for publication on December 16, 2020. Acknowledgments.- We would like to acknowledge Gonzalo Escayola, the
ranchers, the staff of the slaughterhouse and the interns who...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Tacuarembó. |
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28. | | SCHILD, C.; OLIVEIRA, L.G.S.; MIRABALLES, C.; GIANNITTI, F.; CASAUX, M.L.; ARÁOZ, V.; BOABAID, F.M; RIET-CORREA, F. Baccharis coridifolia poisoning in livestock in Uruguay. Toxicon, December 2020, Volume 188, Pages 5-10. Doi: https://doi.org/10.1016/j.toxicon.2020.10.006 Article history: Received 14 August 2020/ Revised 29 September 2020/ Accepted 5 October 2020/ Available online 10 October 2020. Corresponding author. E-mail address: franklin.riet@pq.cnpq.br (F. Riet-Correa).Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela; INIA Tacuarembó. |
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29. | | MEDEIROS, M.A.DE; BARROS, A.T. M. DE; RIET-CORREA, F.; MARQUES, A.R.; LOPES, J.R.G.; VIEIRA, V.D.; MIRABALLES, C. Identification of Sindhi cows that are susceptible or resistant to Haematobia irritans. Braz. J. Vet. Parasitol., 2019. OPEN ACCESS. Article history: Received April 07, 2019; Accepted July 25, 2019.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Tacuarembó. |
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30. | | PARODI, P.; CORBELLINI, L.G.; LEOTTI, V.B.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M.; ARMÚA-FERNÁNDEZ, M.T. Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Full Scientific Report) Journal of Veterinary Diagnostic Investigation, Volume 33, Issue 1, January 2021, Pages 73-79. DOI: https://doi.org/10.1177/1040638720975742 Article history: Article first published online: November 30, 2020; Issue published: January 1, 2021. Acknowledgments: We thank Dra. María Angélica Solari (DILAVE-Montevideo), and the veterinarians and farmers who submitted samples....Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela; INIA Tacuarembó. |
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Registros recuperados : 30 | |
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