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| Registros recuperados : 7 | |
| 1. |  | DINI, M.; FRANZON, R.C.; RASEIRA, M.C.B; UENO, B.; MARCHI, P.M.; VIZZOTTO, M. Blossom blight resistance in peach: phenotyping and antioxidants content in petals. Brazilian Archives of Biology and Technology, 2023, Volume 66, e23220730. https://doi.org/10.1590/1678-4324-2023220730 -- OPEN ACCESS. Article history: Received 15 September 2022, Accepted 31 May 2023, Publication in this collection 30 October 2023, Date of issue 2023. -- Document type: Article Gold Open Access. -- Correspondence: Dini, M.; Universidade Federal de...| Biblioteca(s): INIA Las Brujas. |
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| 3. | | DINI, M.; RASEIRA, M. DO C. B.; SCARIOTTO, S.; MARCHI, P. M.; MELLO-FARIAS, P. Peach phenological characters: heritability, maternal effect and correlation with brown rot. Genetics and Molecular Research, 2021, Volume 20, Issue 1, Article number gmr18684, Pages 1-12. OPEN ACCESS. DOI: https://doi.org/10.4238/gmr18684 Article history: Received November 19, 2020; Accepted December 15, 2020; Published January 31, 2021.
This study was partially funded by the Agencia Nacional de Investigación e Innovación -Uruguay (ANII) in the first year and by the...| Biblioteca(s): INIA Las Brujas. |
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| 5. | | ABREU, E. S.; DINI, M.; CARRA, B.; MARCHI, P. M.; HERTER, F. G.; MELLO-FARIAS, P. C. Bud break promoters following different chilling hour accumulation of 'Hosui' pear. [Conference paper]. Acta Horticulturae, February 2021, N°1303, p. 299-304. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.42 Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein.| Biblioteca(s): INIA Las Brujas. |
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| 6. | | MARCHI, P. M.; CARVALHO, R.; HÖHN, D.; CARRA, B.; SZARESKI, V. J.; DINI, M.; VIZZOTTO, M.; ANTUNES, L. E. C. Genetic parameters prediction (REML/BLUP) for morphological, physical-chemical, phytochemical, and mineral components of primocane-fruiting raspberries. Genetics and Molecular Research, 2019, Volume 18, Issue 2, 2019, Article number gmr18254. OPEN ACCESS. DOI: http://dx.doi.org/10.4238/gmr18254 Article history: Received February 04, 2019; Accepted May 17, 2019; Published May 24, 2019.| Biblioteca(s): INIA Las Brujas. |
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| 7. | | MARCHI, P. M.; CARVALHO, I. R; DINI, M.; SZARESKI, V. J.; PELEGRIN, A. J. DE; PEREIRA, I. S.; ANTUNES, L. E. C. Canonical correlations among morphological and yield traits in primocane-fruiting raspberries. Genetics and Molecular Research, 30 June 2020, Volume 19, Issue 2, Article number gmr18500, Pages 1-7. OPEN ACCESS. DOI: http://dx.doi.org/10.4238/gmr18500 Article history: Received October 11, 2019; Accepted May 28, 2020; Published June 30, 2020.
Corresponding author: Carvalho, I.R.; Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Ijuí, RS, Brazil;...| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 7 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
13/07/2023 |
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Actualizado : |
13/07/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DOS SANTOS-NETO, P.C.; CUADRO, F.; SOUZA-NEVES, M.; CRISPO, M.; MENCHACA, A. |
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Afiliación : |
P.C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; F. CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; M. SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Uruguay; M. CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Refinements in embryo manipulation applied to CRISPR technology in livestock. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Theriogenology. 2023, Volume 208, Pages 142-148. https://doi.org/10.1016/j.theriogenology.2023.05.028 |
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ISSN : |
0093-691X |
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DOI : |
10.1016/j.theriogenology.2023.05.028 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 14 April 2023; Received in revised form 29 May 2023; Accepted 29 May 2023; Available online 9 June 2023. -- Correspondence author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; email:menchaca.alejo@gmail.com -- FUNDING: This study was financially supported by Fundación IRAUy, FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11, and the Uruguayan National Research Agency (ANII, Uruguay). PCdSN received a scholarship from the National Council for Scientific Technological Development (CNPq, Brazil). AM, MC, and PCdSN are fellows of Sistema Nacional de Investigadores (SNI, ANII) of Uruguay and PEDECIBA. -- |
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Contenido : |
The implementation of CRISPR technology in large animals requires further improvements in embryo manipulation and transfer to be applied with commercial purposes. In this study we report (a) developmental competence of CRISPR/Cas microinjected zygotes subjected to in vitro culture in large scale programs in sheep; (b) pregnancy outcomes after early-stage (2-8-cell) embryo transfer into the oviduct or the uterine horn; and (c) embryo survival and birth rate after vitrification/warming of CRISPR/Cas microinjected zygotes. Experiment 1 consisted of a retrospective analysis to evaluate embryo developmental rate of in vitro produced zygotes subjected to CRISPR/Cas microinjection (n = 7,819) compared with a subset of non-microinjected zygotes (n = 701). Development rates to blastocyst on Day 6 were 20.0% for microinjected zygotes and 44.9% for non-injected zygotes (P < 0.05). In Experiment 2, CRISPR/Cas microinjected zygotes were transferred on Day 2 after in vitro fertilization (2-8 cell embryos) into the oviductal ampulla (n = 262) or into the uterine horn (n = 276) in synchronized recipient ewes at prefixed time (i.e., approximately two days after ovulation). Pregnant/transferred recipients (24.0% vs. 25.0%), embryo survival/transferred embryos (6.9% vs. 6.2%), and born lambs/pregnant embryos (72.2% vs. 100.0%) did not differ significantly in the two groups. In Experiment 3, CRISPR/Cas microinjected zygotes were maintained under in vitro culture until blastocyst stage (Day 6), and subjected to vitrification/warming via the Cryotop method (n = 474), while a subset of embryos were left fresh as control group (n = 75). Embryos were transferred into the uterine horn of recipient females at prefixed time 8.5 days after the estrous synchronization treatment (i.e., approximately six days after ovulation). Pregnancy rate (30.8% vs. 48.0%), embryo survival rate (14.8% vs. 21.3%), and birth rate (85.7% vs. 75.0%) were not different (P[dbnd]NS) between vitrified and fresh embryos, respectively. In conclusion, the current study in sheep embryos reports (a) suitable developmental rate after CRISPR/Cas microinjection (i.e., 20%), even though it was lower than non-microinjected zygotes; (b) similar outcomes when Day 2-embryos were placed into the uterine horn instead of the oviduct, avoiding both time-consuming and invasive oviduct manipulation, and extended in vitro culture during one week; (c) promising pregnancy and birth rates obtained with vitrification of CRISPR/Cas microinjected embryos. This knowledge on in vitro embryo development, timing of embryo transfer, and cryopreservation of CRISPR/Cas microinjected zygotes have practical implications for the implementation of genome editing technology in large animals. © 2023 Elsevier Inc. MenosThe implementation of CRISPR technology in large animals requires further improvements in embryo manipulation and transfer to be applied with commercial purposes. In this study we report (a) developmental competence of CRISPR/Cas microinjected zygotes subjected to in vitro culture in large scale programs in sheep; (b) pregnancy outcomes after early-stage (2-8-cell) embryo transfer into the oviduct or the uterine horn; and (c) embryo survival and birth rate after vitrification/warming of CRISPR/Cas microinjected zygotes. Experiment 1 consisted of a retrospective analysis to evaluate embryo developmental rate of in vitro produced zygotes subjected to CRISPR/Cas microinjection (n = 7,819) compared with a subset of non-microinjected zygotes (n = 701). Development rates to blastocyst on Day 6 were 20.0% for microinjected zygotes and 44.9% for non-injected zygotes (P < 0.05). In Experiment 2, CRISPR/Cas microinjected zygotes were transferred on Day 2 after in vitro fertilization (2-8 cell embryos) into the oviductal ampulla (n = 262) or into the uterine horn (n = 276) in synchronized recipient ewes at prefixed time (i.e., approximately two days after ovulation). Pregnant/transferred recipients (24.0% vs. 25.0%), embryo survival/transferred embryos (6.9% vs. 6.2%), and born lambs/pregnant embryos (72.2% vs. 100.0%) did not differ significantly in the two groups. In Experiment 3, CRISPR/Cas microinjected zygotes were maintained under in vitro culture until blastocyst stage (Day 6), ... Presentar Todo |
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Palabras claves : |
Early embryo transfer; Gene editing; In vitro embryo production; Minimum volume vitrification. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
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100 1 $aDOS SANTOS-NETO, P.C.
245 $aRefinements in embryo manipulation applied to CRISPR technology in livestock.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 14 April 2023; Received in revised form 29 May 2023; Accepted 29 May 2023; Available online 9 June 2023. -- Correspondence author: Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; email:menchaca.alejo@gmail.com -- FUNDING: This study was financially supported by Fundación IRAUy, FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11, and the Uruguayan National Research Agency (ANII, Uruguay). PCdSN received a scholarship from the National Council for Scientific Technological Development (CNPq, Brazil). AM, MC, and PCdSN are fellows of Sistema Nacional de Investigadores (SNI, ANII) of Uruguay and PEDECIBA. --
520 $aThe implementation of CRISPR technology in large animals requires further improvements in embryo manipulation and transfer to be applied with commercial purposes. In this study we report (a) developmental competence of CRISPR/Cas microinjected zygotes subjected to in vitro culture in large scale programs in sheep; (b) pregnancy outcomes after early-stage (2-8-cell) embryo transfer into the oviduct or the uterine horn; and (c) embryo survival and birth rate after vitrification/warming of CRISPR/Cas microinjected zygotes. Experiment 1 consisted of a retrospective analysis to evaluate embryo developmental rate of in vitro produced zygotes subjected to CRISPR/Cas microinjection (n = 7,819) compared with a subset of non-microinjected zygotes (n = 701). Development rates to blastocyst on Day 6 were 20.0% for microinjected zygotes and 44.9% for non-injected zygotes (P < 0.05). In Experiment 2, CRISPR/Cas microinjected zygotes were transferred on Day 2 after in vitro fertilization (2-8 cell embryos) into the oviductal ampulla (n = 262) or into the uterine horn (n = 276) in synchronized recipient ewes at prefixed time (i.e., approximately two days after ovulation). Pregnant/transferred recipients (24.0% vs. 25.0%), embryo survival/transferred embryos (6.9% vs. 6.2%), and born lambs/pregnant embryos (72.2% vs. 100.0%) did not differ significantly in the two groups. In Experiment 3, CRISPR/Cas microinjected zygotes were maintained under in vitro culture until blastocyst stage (Day 6), and subjected to vitrification/warming via the Cryotop method (n = 474), while a subset of embryos were left fresh as control group (n = 75). Embryos were transferred into the uterine horn of recipient females at prefixed time 8.5 days after the estrous synchronization treatment (i.e., approximately six days after ovulation). Pregnancy rate (30.8% vs. 48.0%), embryo survival rate (14.8% vs. 21.3%), and birth rate (85.7% vs. 75.0%) were not different (P[dbnd]NS) between vitrified and fresh embryos, respectively. In conclusion, the current study in sheep embryos reports (a) suitable developmental rate after CRISPR/Cas microinjection (i.e., 20%), even though it was lower than non-microinjected zygotes; (b) similar outcomes when Day 2-embryos were placed into the uterine horn instead of the oviduct, avoiding both time-consuming and invasive oviduct manipulation, and extended in vitro culture during one week; (c) promising pregnancy and birth rates obtained with vitrification of CRISPR/Cas microinjected embryos. This knowledge on in vitro embryo development, timing of embryo transfer, and cryopreservation of CRISPR/Cas microinjected zygotes have practical implications for the implementation of genome editing technology in large animals. © 2023 Elsevier Inc.
653 $aEarly embryo transfer
653 $aGene editing
653 $aIn vitro embryo production
653 $aMinimum volume vitrification
700 1 $aCUADRO, F.
700 1 $aSOUZA-NEVES, M.
700 1 $aCRISPO, M.
700 1 $aMENCHACA, A.
773 $tTheriogenology. 2023, Volume 208, Pages 142-148. https://doi.org/10.1016/j.theriogenology.2023.05.028
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