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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
24/10/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
ISSN : |
1948-5948 |
DOI : |
10.4172/1948-5948.1000155 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500 001 1051279 005 2019-10-15 008 2014 bl uuuu u00u1 u #d 022 $a1948-5948 024 7 $a10.4172/1948-5948.1000155$2DOI 100 1 $aALEM, D. 245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource] 260 $c2014 520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. 650 $aACTIVIDAD ANTIMICROBIANA 650 $aALTERNARIA 650 $aBIOTECNOLOGÍA 650 $aPÉPTIDOS 700 1 $aDIAZ, P. 700 1 $aLEONI, C. 700 1 $aDE SIMONE, S.G. 700 1 $aCORREA, A. 700 1 $aOPPEZZO, P. 700 1 $aDALLA RIZZA, M. 773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
05/12/2018 |
Actualizado : |
05/12/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
QUERO, G.; BONNECARRERE, V.; FERNÁNDEZ, S.; SILVA, P.; SIMONDI, S.; BORSANI, O. |
Afiliación : |
GASTÓN QUERO CORRALLO, Universidad de la República (UdelaR)/ Facultad de Agronomía; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SEBASTIÁN FERNÁNDEZ, Universidad de la República (UdelaR)/ Facultad de Ingeniería; PEDRO SILVA, Universidad de la República (UdelaR)/ Facultad de Agronomía; SEBASTIÁN SIMONDI, Universidad Nacional de Cuyo (FCEN-UNCuyo), Argentina; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
Título : |
Light-use efficiency and energy partitioning in rice is cultivar dependent. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Photosynthesis Research, 2018. |
ISSN : |
0166-8595 |
DOI : |
10.1007/s11120-018-0605-x |
Idioma : |
Inglés |
Notas : |
Article history: Received 29 August 2018 // Accepted 12 November 2018 // First Online 17 November 2018.
Electronic supplementary material: The online version of this article contains supplementary material, which is available to authorized users.
Acknowledgements: The authors would like to acknowledge the Agencia Nacional de Investigación e Innovación (ANII) which funded the Doctoral Scholarship of Gaston Quero (POS_NAC_2012_1_8560). This research was also funded by an INIA Project named “Desarrollo de una plataforma de fenotipado como base para la mejora de la tolerancia a estrés ambiental de cultivos y ajuste de modelos de simulación” (L4_10_1_AZ_BT_GT1_8850), and by Grupo Comisión Sectorial de Investigación Científica (CSIC I+D 418). |
Contenido : |
ABSTRACT.
One of the main limitations of rice yield in regions of high productive performance is the light-use efficiency (LUE). LUE can be determined at the whole-plant level or at the photosynthetic apparatus level (quantum yield). Both vary according to the intensity and spectral quality of light. The aim of this study was to analyze the cultivar dependence regarding LUE at the plant level and quantum yield using four rice cultivars and four light environments. To achieve this, two in-house Light Systems were developed: Light System I which generates white light environments (spectral quality of 400?700 nm band) and Light System II which generates a blue-red light environment (spectral quality of 400?500 nm and 600?700 nm bands). Light environment conditioned the LUE and quantum yield in PSII of all evaluated cultivars. In white environments, LUE decreased when light intensity duplicated, while in blue-red environments no differences on LUE were observed. Energy partition in PSII was determined by the quantum yield of three de-excitation processes using chlorophyll fluorescence parameters. For this purpose, a quenching analysis followed by a relaxation analysis was performed. The damage of PSII was only increased by low levels of energy in white environments, leading to a decrease in photochemical processes due to the closure of the reaction centers. In conclusion, all rice cultivars evaluated in this study were sensible to low levels of radiation, but the response was cultivar dependent. There was not a clear genotypic relation between LUE and quantum yield.
© 2018, Springer Nature B.V. MenosABSTRACT.
One of the main limitations of rice yield in regions of high productive performance is the light-use efficiency (LUE). LUE can be determined at the whole-plant level or at the photosynthetic apparatus level (quantum yield). Both vary according to the intensity and spectral quality of light. The aim of this study was to analyze the cultivar dependence regarding LUE at the plant level and quantum yield using four rice cultivars and four light environments. To achieve this, two in-house Light Systems were developed: Light System I which generates white light environments (spectral quality of 400?700 nm band) and Light System II which generates a blue-red light environment (spectral quality of 400?500 nm and 600?700 nm bands). Light environment conditioned the LUE and quantum yield in PSII of all evaluated cultivars. In white environments, LUE decreased when light intensity duplicated, while in blue-red environments no differences on LUE were observed. Energy partition in PSII was determined by the quantum yield of three de-excitation processes using chlorophyll fluorescence parameters. For this purpose, a quenching analysis followed by a relaxation analysis was performed. The damage of PSII was only increased by low levels of energy in white environments, leading to a decrease in photochemical processes due to the closure of the reaction centers. In conclusion, all rice cultivars evaluated in this study were sensible to low levels of radiation, but the response was cu... Presentar Todo |
Palabras claves : |
ENERGY DISSIPATION; QUANTUM YIELDS; QUENCHING ANALYSES; RELAXATION ANALYSES. |
Asunto categoría : |
-- |
Marc : |
LEADER 03097naa a2200265 a 4500 001 1059334 005 2018-12-05 008 2018 bl uuuu u00u1 u #d 022 $a0166-8595 024 7 $a10.1007/s11120-018-0605-x$2DOI 100 1 $aQUERO, G. 245 $aLight-use efficiency and energy partitioning in rice is cultivar dependent.$h[electronic resource] 260 $c2018 500 $aArticle history: Received 29 August 2018 // Accepted 12 November 2018 // First Online 17 November 2018. Electronic supplementary material: The online version of this article contains supplementary material, which is available to authorized users. Acknowledgements: The authors would like to acknowledge the Agencia Nacional de Investigación e Innovación (ANII) which funded the Doctoral Scholarship of Gaston Quero (POS_NAC_2012_1_8560). This research was also funded by an INIA Project named “Desarrollo de una plataforma de fenotipado como base para la mejora de la tolerancia a estrés ambiental de cultivos y ajuste de modelos de simulación” (L4_10_1_AZ_BT_GT1_8850), and by Grupo Comisión Sectorial de Investigación Científica (CSIC I+D 418). 520 $aABSTRACT. One of the main limitations of rice yield in regions of high productive performance is the light-use efficiency (LUE). LUE can be determined at the whole-plant level or at the photosynthetic apparatus level (quantum yield). Both vary according to the intensity and spectral quality of light. The aim of this study was to analyze the cultivar dependence regarding LUE at the plant level and quantum yield using four rice cultivars and four light environments. To achieve this, two in-house Light Systems were developed: Light System I which generates white light environments (spectral quality of 400?700 nm band) and Light System II which generates a blue-red light environment (spectral quality of 400?500 nm and 600?700 nm bands). Light environment conditioned the LUE and quantum yield in PSII of all evaluated cultivars. In white environments, LUE decreased when light intensity duplicated, while in blue-red environments no differences on LUE were observed. Energy partition in PSII was determined by the quantum yield of three de-excitation processes using chlorophyll fluorescence parameters. For this purpose, a quenching analysis followed by a relaxation analysis was performed. The damage of PSII was only increased by low levels of energy in white environments, leading to a decrease in photochemical processes due to the closure of the reaction centers. In conclusion, all rice cultivars evaluated in this study were sensible to low levels of radiation, but the response was cultivar dependent. There was not a clear genotypic relation between LUE and quantum yield. © 2018, Springer Nature B.V. 653 $aENERGY DISSIPATION 653 $aQUANTUM YIELDS 653 $aQUENCHING ANALYSES 653 $aRELAXATION ANALYSES 700 1 $aBONNECARRERE, V. 700 1 $aFERNÁNDEZ, S. 700 1 $aSILVA, P. 700 1 $aSIMONDI, S. 700 1 $aBORSANI, O. 773 $tPhotosynthesis Research, 2018.
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