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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/01/2020 |
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Actualizado : |
24/02/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RODRIGO, M.J.; LADO, J.; ALÓS, E.; ALQUÉZAR, B.; DERY, O.; HIRSCHBERG, J.; ZACARÍAS, L. |
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Afiliación : |
MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; ENRIQUETA ALÓS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; BERTA ALQUÉZAR, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Spain; ORLY DERY, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; JOSEPH HIRSCHBERG, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain. |
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Título : |
A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
BMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2 |
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ISSN : |
1471-2229 |
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DOI : |
10.1186/s12870-019-2078-2 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019.
Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
Supplementary information accompanies this paper. |
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Contenido : |
Abstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits.
© 2019 The Author(s). MenosAbstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit an... Presentar Todo |
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Palabras claves : |
Carotenoid; GENE EXPRESSION; MUTANT; Pigmentation; PLATAFORMA AGROALIMENTOS; RIPENING; Zeta-carotene isomerase. |
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Thesagro : |
CAROTENOIDES; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14051/1/BMC-Plant-Pathology-2019-s12870-019-2078-2.pdf
https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-019-2078-2
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Marc : |
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001 1060661
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022 $a1471-2229
024 7 $a10.1186/s12870-019-2078-2$2DOI
100 1 $aRODRIGO, M.J.
245 $aA mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019. Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Supplementary information accompanies this paper.
520 $aAbstract. Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits. © 2019 The Author(s).
650 $aCAROTENOIDES
650 $aCITRUS
653 $aCarotenoid
653 $aGENE EXPRESSION
653 $aMUTANT
653 $aPigmentation
653 $aPLATAFORMA AGROALIMENTOS
653 $aRIPENING
653 $aZeta-carotene isomerase
700 1 $aLADO, J.
700 1 $aALÓS, E.
700 1 $aALQUÉZAR, B.
700 1 $aDERY, O.
700 1 $aHIRSCHBERG, J.
700 1 $aZACARÍAS, L.
773 $tBMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
02/06/2017 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
DÍAZ, M.T; ÁLVAREZ, I.; DE LA FUENTE, J.; SAÑUDO, C.; CAMPO, M.M; OLIVER, M.A.; FONT I FURNOLS, M.; MONTOSSI, F.; SAN JULIÁN, R.; BRANSCHEID, W.; NUTE, G. R.; CAÑEQUE, V. |
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Afiliación : |
FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO SAN JULIAN SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Fatty acid composition of lamb meat from Spain, Britain, Germany and Uruguay. |
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Fecha de publicación : |
2004 |
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Fuente / Imprenta : |
In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY (ICoMST), 50., 2004, Helsinki, Finland. Proceedings. Helsinki, 2004. |
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Idioma : |
Inglés |
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Contenido : |
The fatty acid composition of intramuscular fat can be influenced by factors such as diet (Rhee, 2000), breed (Robelin, 1986), age (Link et al., 1970) and the level of fatness of animals (Nürnberg et al., 1998). On the other hand, the fatty acid composition influences the nutritive value and the palatability of the meat. In
relation to the nutritive value, consumption of saturated fatty acids (SFA) has been associated with an increase of plasma cholesterol and plasma low density lipoprotein levels, which are linked at the same time to a major risk of coronary heart disease. Conjugated linoleic acid (CLA), which arises from microbial hydrogenation of dietary linoleic acid in the rumen, exhibits anticarcinogenic properties when is included in the diet at low levels (French et al., 2000). Additionally, meat flavour is influenced by the saturation rate of fatty acids (Purchas et al., 1979). The polyunsaturated fatty acids (PUFA) are more susceptible to oxidation than monounsaturated fatty acids (MUFA), their oxidation is primarily responsible for the oxidative meat flavour deterioration (Gatellier et al,, 2001). |
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Palabras claves : |
FATTY ACID COMPOSITION; LAMB MEAT. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6810/1/ICOMST-2004.pdf
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Marc : |
LEADER 01923nam a2200265 a 4500
001 1023403
005 2017-06-02
008 2004 bl uuuu u01u1 u #d
100 1 $aDÍAZ, M.T
245 $aFatty acid composition of lamb meat from Spain, Britain, Germany and Uruguay.
260 $aIn: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY (ICoMST), 50., 2004, Helsinki, Finland. Proceedings. Helsinki$c2004
520 $aThe fatty acid composition of intramuscular fat can be influenced by factors such as diet (Rhee, 2000), breed (Robelin, 1986), age (Link et al., 1970) and the level of fatness of animals (Nürnberg et al., 1998). On the other hand, the fatty acid composition influences the nutritive value and the palatability of the meat. In relation to the nutritive value, consumption of saturated fatty acids (SFA) has been associated with an increase of plasma cholesterol and plasma low density lipoprotein levels, which are linked at the same time to a major risk of coronary heart disease. Conjugated linoleic acid (CLA), which arises from microbial hydrogenation of dietary linoleic acid in the rumen, exhibits anticarcinogenic properties when is included in the diet at low levels (French et al., 2000). Additionally, meat flavour is influenced by the saturation rate of fatty acids (Purchas et al., 1979). The polyunsaturated fatty acids (PUFA) are more susceptible to oxidation than monounsaturated fatty acids (MUFA), their oxidation is primarily responsible for the oxidative meat flavour deterioration (Gatellier et al,, 2001).
653 $aFATTY ACID COMPOSITION
653 $aLAMB MEAT
700 1 $aÁLVAREZ, I.
700 1 $aDE LA FUENTE, J.
700 1 $aSAÑUDO, C.
700 1 $aCAMPO, M.M
700 1 $aOLIVER, M.A.
700 1 $aFONT I FURNOLS, M.
700 1 $aMONTOSSI, F.
700 1 $aSAN JULIÁN, R.
700 1 $aBRANSCHEID, W.
700 1 $aNUTE, G. R.
700 1 $aCAÑEQUE, V.
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INIA Tacuarembó (TBO) |
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