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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
13/11/2015 |
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Actualizado : |
25/09/2016 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LADO, J.; RODRIGO, M.J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Transcriptional regulation of ethylene metabolism in 'Marsh' and 'Star Ruby' grapefruits under chilling conditions. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Acta Horticulturae, 2015, no.1079, p. 501-506. |
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ISBN : |
978-946261071-2 |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2015.1079.66 |
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Idioma : |
Inglés |
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Notas : |
Proc. Vth International Conference Postharvest Unlimited. Eds.: Toivonen P.M.,Kalaitzis P.,Manganaris G.A. |
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Contenido : |
ABSTRACT.
Grapefruits are highly susceptible to developing chilling injury (CI) symptoms during postharvest storage at low temperatures. The role of ethylene in the response of citrus fruits to low temperature is still controversial and whether the hormone is a defense response to cope with the cold-induced damage is not fully understood. Moreover, little is known about the ethylene signaling cascade in coldsensitive citrus fruit under chilling conditions. Therefore, the aim of this work was to study the transcriptional regulation of ethylene biosynthesis, perception and response during storage at 2°C of the yellow ?Marsh? and the red ?Star Ruby? grapefruits. To better understand the regulation of cold- or chilling-induced ethylene production, the effect of the ethylene action inhibitor, 1-MCP, on the expression of ethylene biosynthetic and perception genes was also analyzed. Cold
storage markedly stimulated the expression of ethylene biosynthetic genes; ACC synthase 1 and 2 (ACS1 and ACS2) and ACC oxidase (ACO) in the peel of both cultivars. Expression of ACS1, ACS2 and ACO were overstimulated in the peel of 1-MCP-treated fruit, indicating a negative feedback control of ethylene on the process. Expression of three members of the ETR gene family was also affected by low temperature, but expression of ETR3 gene experienced the major induction by cold stress in the peel of fruit of both cultivars. Pre-treatment with 1-MCP did not show a clear and consistent effect on the expression of the ETR genes. These results illustrate the complex regulation of the different members of ethylene biosynthetic, signaling and response pathway by cold stress in the peel of grapefruits. MenosABSTRACT.
Grapefruits are highly susceptible to developing chilling injury (CI) symptoms during postharvest storage at low temperatures. The role of ethylene in the response of citrus fruits to low temperature is still controversial and whether the hormone is a defense response to cope with the cold-induced damage is not fully understood. Moreover, little is known about the ethylene signaling cascade in coldsensitive citrus fruit under chilling conditions. Therefore, the aim of this work was to study the transcriptional regulation of ethylene biosynthesis, perception and response during storage at 2°C of the yellow ?Marsh? and the red ?Star Ruby? grapefruits. To better understand the regulation of cold- or chilling-induced ethylene production, the effect of the ethylene action inhibitor, 1-MCP, on the expression of ethylene biosynthetic and perception genes was also analyzed. Cold
storage markedly stimulated the expression of ethylene biosynthetic genes; ACC synthase 1 and 2 (ACS1 and ACS2) and ACC oxidase (ACO) in the peel of both cultivars. Expression of ACS1, ACS2 and ACO were overstimulated in the peel of 1-MCP-treated fruit, indicating a negative feedback control of ethylene on the process. Expression of three members of the ETR gene family was also affected by low temperature, but expression of ETR3 gene experienced the major induction by cold stress in the peel of fruit of both cultivars. Pre-treatment with 1-MCP did not show a clear and consistent effect on the expr... Presentar Todo |
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Thesagro : |
CITRUS; ESTRÉS DE FRÍO; ETILENO; TECNOLOGIA DE POSTCOSECHA; TRASTORNOS FUNCIONALES. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02533nga a2200253 a 4500
001 1053876
005 2016-09-25
008 2015 bl uuuu u00u1 u #d
020 $a978-946261071-2
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2015.1079.66$2DOI
100 1 $aLADO, J.
245 $aTranscriptional regulation of ethylene metabolism in 'Marsh' and 'Star Ruby' grapefruits under chilling conditions.
260 $c2015
500 $aProc. Vth International Conference Postharvest Unlimited. Eds.: Toivonen P.M.,Kalaitzis P.,Manganaris G.A.
520 $aABSTRACT. Grapefruits are highly susceptible to developing chilling injury (CI) symptoms during postharvest storage at low temperatures. The role of ethylene in the response of citrus fruits to low temperature is still controversial and whether the hormone is a defense response to cope with the cold-induced damage is not fully understood. Moreover, little is known about the ethylene signaling cascade in coldsensitive citrus fruit under chilling conditions. Therefore, the aim of this work was to study the transcriptional regulation of ethylene biosynthesis, perception and response during storage at 2°C of the yellow ?Marsh? and the red ?Star Ruby? grapefruits. To better understand the regulation of cold- or chilling-induced ethylene production, the effect of the ethylene action inhibitor, 1-MCP, on the expression of ethylene biosynthetic and perception genes was also analyzed. Cold storage markedly stimulated the expression of ethylene biosynthetic genes; ACC synthase 1 and 2 (ACS1 and ACS2) and ACC oxidase (ACO) in the peel of both cultivars. Expression of ACS1, ACS2 and ACO were overstimulated in the peel of 1-MCP-treated fruit, indicating a negative feedback control of ethylene on the process. Expression of three members of the ETR gene family was also affected by low temperature, but expression of ETR3 gene experienced the major induction by cold stress in the peel of fruit of both cultivars. Pre-treatment with 1-MCP did not show a clear and consistent effect on the expression of the ETR genes. These results illustrate the complex regulation of the different members of ethylene biosynthetic, signaling and response pathway by cold stress in the peel of grapefruits.
650 $aCITRUS
650 $aESTRÉS DE FRÍO
650 $aETILENO
650 $aTECNOLOGIA DE POSTCOSECHA
650 $aTRASTORNOS FUNCIONALES
700 1 $aRODRIGO, M.J.
700 1 $aZACARÍAS, L.
773 $tActa Horticulturae, 2015, no.1079, p. 501-506.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
30/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
MORON, A.; COZZOLINO, D. |
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Afiliación : |
DAVID ALEJANDRO MORON YACOEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL COZZOLINO GÓMEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Determination of macro elements in alfalfa and white clover by near-infrared reflectance spectroscopy. |
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Fecha de publicación : |
2002 |
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Fuente / Imprenta : |
Journal of Agricultural Science, December 2002, Volume 139, Issue 4, pages 413-423. |
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DOI : |
10.1017/S0021859602002605 |
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Idioma : |
Inglés |
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Notas : |
Article history: Revised MS received 19 August 2002. |
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Contenido : |
Summary: Near-infrared reflectance spectroscopy was used to assess the mineral composition of both alfalfa (Medicago sativa L.) and white clover (Trifolium repens L.). Alfalfa (n=230) and white clover (n=97) plant samples from different locations in Uruguay representing a wide range of soil types were analysed. The samples were scanned for reflectance in a NIRSystems 6500 monochromator (NIRSystems, Silver Spring, MD, USA). Predictive equations were developed using modified partial least squares (MPLS) with cross validation to avoid overfitting. The coefficients of determination in calibration (R_{\rm cal}^{2}) and the standard errors in cross validation (SECV) were 0·93 (SECV: 1·6), 0·95 (SECV: 1·3), 0·93 (SECV: 1·9), 0·88 (SECV: 2·7), 0·82 (SECV: 0·3) and 0·75 (SECV: 4·7) for alfalfa and 0·98 (SECV: 0·8), 0·52 (SECV: 0·8), 0·97 (SECV: 2·7), 0·83 (SECV: 3·1), 0·82 (SECV: 1·9) and 0·45 (SECV: 2·6) for white clover, for N, Ca, K, P, Mg and S in g/kg on a dry weight respectively. Calcium, nitrogen and potassium were well predicted by NIRS in both alfalfa and white clover samples. |
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Palabras claves : |
MAGNESIUM; MINERAL CONTENT. |
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Thesagro : |
CALCIUM; MEDICAGO SATIVA; NIRS; POTASSIUM. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 01852naa a2200229 a 4500
001 1035490
005 2019-09-30
008 2002 bl uuuu u00u1 u #d
024 7 $a10.1017/S0021859602002605$2DOI
100 1 $aMORON, A.
245 $aDetermination of macro elements in alfalfa and white clover by near-infrared reflectance spectroscopy.$h[electronic resource]
260 $c2002
500 $aArticle history: Revised MS received 19 August 2002.
520 $aSummary: Near-infrared reflectance spectroscopy was used to assess the mineral composition of both alfalfa (Medicago sativa L.) and white clover (Trifolium repens L.). Alfalfa (n=230) and white clover (n=97) plant samples from different locations in Uruguay representing a wide range of soil types were analysed. The samples were scanned for reflectance in a NIRSystems 6500 monochromator (NIRSystems, Silver Spring, MD, USA). Predictive equations were developed using modified partial least squares (MPLS) with cross validation to avoid overfitting. The coefficients of determination in calibration (R_{\rm cal}^{2}) and the standard errors in cross validation (SECV) were 0·93 (SECV: 1·6), 0·95 (SECV: 1·3), 0·93 (SECV: 1·9), 0·88 (SECV: 2·7), 0·82 (SECV: 0·3) and 0·75 (SECV: 4·7) for alfalfa and 0·98 (SECV: 0·8), 0·52 (SECV: 0·8), 0·97 (SECV: 2·7), 0·83 (SECV: 3·1), 0·82 (SECV: 1·9) and 0·45 (SECV: 2·6) for white clover, for N, Ca, K, P, Mg and S in g/kg on a dry weight respectively. Calcium, nitrogen and potassium were well predicted by NIRS in both alfalfa and white clover samples.
650 $aCALCIUM
650 $aMEDICAGO SATIVA
650 $aNIRS
650 $aPOTASSIUM
653 $aMAGNESIUM
653 $aMINERAL CONTENT
700 1 $aCOZZOLINO, D.
773 $tJournal of Agricultural Science, December 2002, Volume 139, Issue 4, pages 413-423.
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