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| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
26/09/2014 |
Actualizado : |
11/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ABREO, E.; MARTINEZ, S.; BETTUCCI, L.; LUPO, S. |
Afiliación : |
SEBASTIAN MARTINEZ KOPP, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Phaeomoniella chlamydospora and Phaeoacremonium ssp. in gravevines from Uruguay. |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
Phytopathologia Mediterranea. 2011. v.50 (Supplement), p.77-85. |
ISSN : |
1593-2095 |
DOI : |
10.14601/Phytopathol_Mediterr-8682 |
Idioma : |
Inglés |
Notas : |
Article history: accepted for publication: February 15, 2010; published 2011.
http://dx.doi.org/10.14601/Phytopathol_Mediterr-8682 |
Contenido : |
Summary
Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected.
Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other fungi frequently isolated from diseased vine tissues. F2bt/R1bt and Pmo1f/ Pmo2r were selected, and were used in a nested PCR to detect Phaeoacremonium spp. and Pa. chlamydospora in asymptomatic canes of nursery mother grapevines. Nine out of ten sampled grapevines tested positive for one
of the fungi. Molecular diagnosis is potentially a useful method to assess the health of mother grapevines. MenosSummary
Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected.
Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other ... Presentar Todo |
Palabras claves : |
ASYMPTOMATIC CANES; ESCA; MONITORING; PETRI DISEASE; SPECIFIC PRIMERS. |
Thesagro : |
ENFERMEDADES DE LA VID; ENFERMEDADES DE LAS PLANTAS; URUGUAY. |
Asunto categoría : |
H20 Enfermedades de las plantas |
Marc : |
LEADER 02850naa a2200289 a 4500 001 1050566 005 2019-10-11 008 2011 bl uuuu u00u1 u #d 022 $a1593-2095 024 7 $a10.14601/Phytopathol_Mediterr-8682$2DOI 100 1 $aABREO, E. 245 $aPhaeomoniella chlamydospora and Phaeoacremonium ssp. in gravevines from Uruguay.$h[electronic resource] 260 $c2011 500 $aArticle history: accepted for publication: February 15, 2010; published 2011. http://dx.doi.org/10.14601/Phytopathol_Mediterr-8682 520 $aSummary Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected. Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other fungi frequently isolated from diseased vine tissues. F2bt/R1bt and Pmo1f/ Pmo2r were selected, and were used in a nested PCR to detect Phaeoacremonium spp. and Pa. chlamydospora in asymptomatic canes of nursery mother grapevines. Nine out of ten sampled grapevines tested positive for one of the fungi. Molecular diagnosis is potentially a useful method to assess the health of mother grapevines. 650 $aENFERMEDADES DE LA VID 650 $aENFERMEDADES DE LAS PLANTAS 650 $aURUGUAY 653 $aASYMPTOMATIC CANES 653 $aESCA 653 $aMONITORING 653 $aPETRI DISEASE 653 $aSPECIFIC PRIMERS 700 1 $aMARTINEZ, S. 700 1 $aBETTUCCI, L. 700 1 $aLUPO, S. 773 $tPhytopathologia Mediterranea. 2011.$gv.50 (Supplement), p.77-85.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
26/02/2024 |
Actualizado : |
26/02/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
JÁUREGUI, J.M.; MICHELINI, D.F.; SEVILLA, G.H.; BERHONGARAY, G.; BERONE, G.D.; BAUDRACCO, J.; CHILIBROSTE, P.; AGNUSDEI, M.G.; LATTANZI, F. |
Afiliación : |
J. M JÁUREGUI, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Argentina; Livestock Division, Datamars SA, Lamone, Switzerland; D. F. MICHELINI, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Salto, Uruguay; G. H. SEVILLA, Estación Experimental Agropecuaria Concepción de Uruguay, Instituto Nacional de Tecnología Agropecuaria, Entre Ríos, Concepción del Uruguay, Argentina; G. BERHONGARAY, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Argentina; ICiAgro Litoral, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina; G. D. BERONE, Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Balcarce, Argentina; Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina; J. BAUDRACCO, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Argentina; ICiAgro Litoral, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina; PABLO CHILIBROSTE SYMONDS, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; M. G. AGNUSDEI, Estación Experimental Agropecuaria Balcarce, Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Balcarce, Argentina; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Tall fescue tiller survival over summer in a subtropical environment: The role of the size and depth of root systems. |
Fecha de publicación : |
2024 |
Fuente / Imprenta : |
Journal of Agronomy and Crop Science. 2024, Volume 210, Issue 1, article e12682. https://doi.org/10.1111/jac.12682 |
ISSN : |
0931-2250 |
DOI : |
10.1111/jac.12682 |
Idioma : |
Inglés |
Notas : |
Article history: Received 5 May 2023; Revised 20 October 2023; Accepted 1 November 2023. -- Correspondence: J. M. Jáuregui, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Argentina. Email: josemartinjauregui@gmail.com -- Funding information:
Consejo Nacional de Investigaciones Científicas y Técnicas. -- |
Contenido : |
ABSTRACT.- Pasture persistence is a key determinant of the economic and environmental performance of pastoral animal production systems. Large and deep root systems that help resist summer water stress have been proposed as a relevant trait for vegetative persistence of perennial temperate forage species growing in subtropical climates or under future climatically challenging scenarios. In a previous study [Jauregui et al., 2017. Persistence of tall fescue in a subtropical environment: Tiller survival over summer in response to flowering control and nitrogen supply. Grass and Forage Science 72, 454-466] we have shown that nitrogen fertilization and grazing management aimed at 'control flowering' increased the survival of tall fescue tillers during harsh summers in Uruguay (lat. 32°S). Here we assessed: (i) to what extent tiller survival is mediated by root system size in spring and (ii) what consequences tiller survival entails for root mass, depth and morphology the following autumn. In two field experiments, significant increases in tiller survival in response to nitrogen fertilization and grazing management (+60% and +80% in 2011/12 and 2012/13, respectively) were not related to concomitant effects on the size or depth of the root system in spring (p >.10). Even when six-fold within-treatment variation in root mass was observed, within-treatment variation in summer tiller survival was little affected (<15%, p =.08). In turn, differences in tiller survival over summer affected little root system characteristics the following autumn. Therefore, we found scant support for the hypothesis that large and deep root systems contribute to survival of tall fescue tillers in this subtropical humid climate. Except for soils with less than 30 mm of plant available water holding capacity, summer water deficits did not induce severe tiller mortality in tall fescue in this climate. © 2024 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd. MenosABSTRACT.- Pasture persistence is a key determinant of the economic and environmental performance of pastoral animal production systems. Large and deep root systems that help resist summer water stress have been proposed as a relevant trait for vegetative persistence of perennial temperate forage species growing in subtropical climates or under future climatically challenging scenarios. In a previous study [Jauregui et al., 2017. Persistence of tall fescue in a subtropical environment: Tiller survival over summer in response to flowering control and nitrogen supply. Grass and Forage Science 72, 454-466] we have shown that nitrogen fertilization and grazing management aimed at 'control flowering' increased the survival of tall fescue tillers during harsh summers in Uruguay (lat. 32°S). Here we assessed: (i) to what extent tiller survival is mediated by root system size in spring and (ii) what consequences tiller survival entails for root mass, depth and morphology the following autumn. In two field experiments, significant increases in tiller survival in response to nitrogen fertilization and grazing management (+60% and +80% in 2011/12 and 2012/13, respectively) were not related to concomitant effects on the size or depth of the root system in spring (p >.10). Even when six-fold within-treatment variation in root mass was observed, within-treatment variation in summer tiller survival was little affected (<15%, p =.08). In turn, differences in tiller survival over summer affe... Presentar Todo |
Palabras claves : |
Forage; Grazing management; Heat stress; Nitrogen fertilisation; Partnership for the goals - Goal 17; Pasture persistence; Sustainable Development Goals (SDGs). |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 03371naa a2200337 a 4500 001 1064473 005 2024-02-26 008 2024 bl uuuu u00u1 u #d 022 $a0931-2250 024 7 $a10.1111/jac.12682$2DOI 100 1 $aJÁUREGUI, J.M. 245 $aTall fescue tiller survival over summer in a subtropical environment$bThe role of the size and depth of root systems.$h[electronic resource] 260 $c2024 500 $aArticle history: Received 5 May 2023; Revised 20 October 2023; Accepted 1 November 2023. -- Correspondence: J. M. Jáuregui, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Argentina. Email: josemartinjauregui@gmail.com -- Funding information: Consejo Nacional de Investigaciones Científicas y Técnicas. -- 520 $aABSTRACT.- Pasture persistence is a key determinant of the economic and environmental performance of pastoral animal production systems. Large and deep root systems that help resist summer water stress have been proposed as a relevant trait for vegetative persistence of perennial temperate forage species growing in subtropical climates or under future climatically challenging scenarios. In a previous study [Jauregui et al., 2017. Persistence of tall fescue in a subtropical environment: Tiller survival over summer in response to flowering control and nitrogen supply. Grass and Forage Science 72, 454-466] we have shown that nitrogen fertilization and grazing management aimed at 'control flowering' increased the survival of tall fescue tillers during harsh summers in Uruguay (lat. 32°S). Here we assessed: (i) to what extent tiller survival is mediated by root system size in spring and (ii) what consequences tiller survival entails for root mass, depth and morphology the following autumn. In two field experiments, significant increases in tiller survival in response to nitrogen fertilization and grazing management (+60% and +80% in 2011/12 and 2012/13, respectively) were not related to concomitant effects on the size or depth of the root system in spring (p >.10). Even when six-fold within-treatment variation in root mass was observed, within-treatment variation in summer tiller survival was little affected (<15%, p =.08). In turn, differences in tiller survival over summer affected little root system characteristics the following autumn. Therefore, we found scant support for the hypothesis that large and deep root systems contribute to survival of tall fescue tillers in this subtropical humid climate. Except for soils with less than 30 mm of plant available water holding capacity, summer water deficits did not induce severe tiller mortality in tall fescue in this climate. © 2024 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd. 653 $aForage 653 $aGrazing management 653 $aHeat stress 653 $aNitrogen fertilisation 653 $aPartnership for the goals - Goal 17 653 $aPasture persistence 653 $aSustainable Development Goals (SDGs) 700 1 $aMICHELINI, D.F. 700 1 $aSEVILLA, G.H. 700 1 $aBERHONGARAY, G. 700 1 $aBERONE, G.D. 700 1 $aBAUDRACCO, J. 700 1 $aCHILIBROSTE, P. 700 1 $aAGNUSDEI, M.G. 700 1 $aLATTANZI, F. 773 $tJournal of Agronomy and Crop Science. 2024, Volume 210, Issue 1, article e12682. https://doi.org/10.1111/jac.12682
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