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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
03/12/2020 |
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Actualizado : |
03/12/2020 |
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Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
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Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
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Título : |
Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
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DOI : |
10.1007/s11240-020-01891-6 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
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Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
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Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 03007naa a2200301 a 4500
001 1061539
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11240-020-01891-6$2DOI
100 1 $aNARANCIO, R.
245 $aCorrection to$bApplication of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6).$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020.
520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V.
653 $aAgrobacterium-mediated transformation
653 $aDelayed leaf senescence
653 $aMultigene
653 $aT-DNA
700 1 $aDING, Y.-L.
700 1 $aLIN, Y.-H.
700 1 $aSAHAB, S.
700 1 $aPANTER, S.
700 1 $aHAYES, M.
700 1 $aJOHN, U.
700 1 $aANDERSON, H.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
12/04/2024 |
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Actualizado : |
12/04/2024 |
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Tipo de producción científica : |
Folletos |
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Autor : |
INIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA); UNIVERSIDAD DE LA REPÚBLICA (UDELAR)/ FACULTAD DE AGRONOMÍA |
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Título : |
Guayabo del país (Acca sellowiana (Berg.) Burret). Primeras selecciones registradas en Uruguay. [actualización 2024, folleto]. |
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Fecha de publicación : |
2024 |
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Fuente / Imprenta : |
Montevideo (UY): INIA. |
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Idioma : |
Español |
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Contenido : |
En el año 2000 la Facultad de Agronomía (Udelar), el Instituto Nacional de Investigación Agropecuaria (INIA) y el Ministerio de Ganadería, Agricultura y Pesca (MGAP) comenzaron un programa de selección de frutas nativas con posible potencial comercial. A este trabajo se unieron más tarde otras instituciones y actores sociales. A partir de las evaluaciones que se realizan, se seleccionaron cuatro materiales de guayabo del país que han sido inscriptos en el Registro Nacional de Cultivares de INASE con los nombres fantasía "INIA Fagro Isleña", "INIA Fagro Cerrillana", "INIA Fagro Artillera" e "INIA Fagro Armonía". Todas ellas tienen un potencial superior por sus características de sabor, productividad, estabilidad en la producción, tamaño de fruto y capacidad de propagación vegetativa. Además, se complementan en sus fechas de cosecha y en la posibilidad de su uso como polinizadores, debiéndose usar al menos dos de ellos en los cuadros comerciales a instalarse. |
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Palabras claves : |
INIA Fagro Armonía; INIA Fagro Artillera; INIA Fagro Cerrillana; INIA Fagro Isleña; SELECCIÓN DE VARIEDADES; SISTEMA VEGETAL INTENSIVO - INIA. |
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Thesagro : |
ACCA SELLOWIANA; GUAYABO DEL PAÍS; URUGUAY; VARIEDADES. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17571/1/Guayabo-del-pais-folleto-2024.pdf
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Marc : |
LEADER 01843nam a2200241 a 4500
001 1064553
005 2024-04-12
008 2024 bl uuuu u0uu1 u #d
100 1 $aINIA (INSTITUTO NACIONAL DE INVESTIGACIÓN AGROPECUARIA)
245 $aGuayabo del país (Acca sellowiana (Berg.) Burret). Primeras selecciones registradas en Uruguay. [actualización 2024, folleto].$h[electronic resource]
260 $aMontevideo (UY): INIA.$c2024
520 $aEn el año 2000 la Facultad de Agronomía (Udelar), el Instituto Nacional de Investigación Agropecuaria (INIA) y el Ministerio de Ganadería, Agricultura y Pesca (MGAP) comenzaron un programa de selección de frutas nativas con posible potencial comercial. A este trabajo se unieron más tarde otras instituciones y actores sociales. A partir de las evaluaciones que se realizan, se seleccionaron cuatro materiales de guayabo del país que han sido inscriptos en el Registro Nacional de Cultivares de INASE con los nombres fantasía "INIA Fagro Isleña", "INIA Fagro Cerrillana", "INIA Fagro Artillera" e "INIA Fagro Armonía". Todas ellas tienen un potencial superior por sus características de sabor, productividad, estabilidad en la producción, tamaño de fruto y capacidad de propagación vegetativa. Además, se complementan en sus fechas de cosecha y en la posibilidad de su uso como polinizadores, debiéndose usar al menos dos de ellos en los cuadros comerciales a instalarse.
650 $aACCA SELLOWIANA
650 $aGUAYABO DEL PAÍS
650 $aURUGUAY
650 $aVARIEDADES
653 $aINIA Fagro Armonía
653 $aINIA Fagro Artillera
653 $aINIA Fagro Cerrillana
653 $aINIA Fagro Isleña
653 $aSELECCIÓN DE VARIEDADES
653 $aSISTEMA VEGETAL INTENSIVO - INIA
700 1 $aUNIVERSIDAD DE LA REPÚBLICA (UDELAR)/ FACULTAD DE AGRONOMÍA
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