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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
20/11/2020 |
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Actualizado : |
21/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
KASPARY, T. E.; CUTTI, L.; SCHWALM RAFAELI, R.; ANDREA DELATORRE, C.; MEROTTO JR, A. |
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Afiliación : |
TIAGO EDU KASPARY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUAN CUTTI, Department of Crop Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.; RAFAEL SCHWALM RAFAELI, Department of Crop Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.; CARLA ANDREA DELATORRE, Department of Crop Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.; ALDO MEROTTO JR, Department of Crop Sciences, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. |
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Título : |
Genes related to flooding tolerance during germination and early growth of weedy rice. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Weed Research. 2020, vol. 60, p.435?449. DOI:https://doi.org/10.1111/wre.12448 |
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DOI : |
10.1111/wre.12448 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 10 June 2019/ Accepted: 6 July 2020. |
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Contenido : |
Abstract: Evolution of flooding tolerance in weedy rice has occurred in several rice-growing regions, but the genes related to this process and the environmental effects are unknown. The objective of this study was to analyse the expression of genes related to flooding tolerance in response to temperature and flooding during the initial establishment of weedy rice. The experiments were carried out with rice cultivars IRGA 417 and Nipponbare, which are sensitive to flooding, and weedy rice ITJ03 and AV04 genotypes that have high and intermediate tolerance to flooding, respectively. The expression of genes related to reserve mobilisation, anaerobic respiration, escape and quiescence strategies was analysed at periods up to 24 days after sowing. The flooding tolerance of weedy rice genotype ITJ03 was associated with the expression of RAmy3D and OsTPP7, which are involved in the mobilisation of carbohydrate reserves, ADH1 and ADH2, which participate in anaerobic respiration, and SNRKL1 that triggers rapid elongation of the coleoptile and emergence. Although the genes PDC1,SUS3 and SUB1 are important for flooding tolerance in cultivated rice, their expression was not directly related to flooding tolerance in weedy rice. A temperature of 20°C reduced levels of expression of the RAmy3D, ADH2 and SNRKL1 genes and low temperature had a negative effect on the establishment of weedy rice. Breeding of rice genotypes with tolerance of low temperatures and anaerobic conditions may be a viable strategy to improve the control of weedy rice in paddy fields. MenosAbstract: Evolution of flooding tolerance in weedy rice has occurred in several rice-growing regions, but the genes related to this process and the environmental effects are unknown. The objective of this study was to analyse the expression of genes related to flooding tolerance in response to temperature and flooding during the initial establishment of weedy rice. The experiments were carried out with rice cultivars IRGA 417 and Nipponbare, which are sensitive to flooding, and weedy rice ITJ03 and AV04 genotypes that have high and intermediate tolerance to flooding, respectively. The expression of genes related to reserve mobilisation, anaerobic respiration, escape and quiescence strategies was analysed at periods up to 24 days after sowing. The flooding tolerance of weedy rice genotype ITJ03 was associated with the expression of RAmy3D and OsTPP7, which are involved in the mobilisation of carbohydrate reserves, ADH1 and ADH2, which participate in anaerobic respiration, and SNRKL1 that triggers rapid elongation of the coleoptile and emergence. Although the genes PDC1,SUS3 and SUB1 are important for flooding tolerance in cultivated rice, their expression was not directly related to flooding tolerance in weedy rice. A temperature of 20°C reduced levels of expression of the RAmy3D, ADH2 and SNRKL1 genes and low temperature had a negative effect on the establishment of weedy rice. Breeding of rice genotypes with tolerance of low temperatures and anaerobic conditions may be a vi... Presentar Todo |
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Palabras claves : |
ADAPTABILITY; ESCAPE; HYPOXIA; INVASIVENESS; ORYZA SATIVA L; QUIESCENCE. |
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Thesagro : |
ARROZ; MALEZAS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02437naa a2200289 a 4500
001 1061513
005 2021-05-21
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1111/wre.12448$2DOI
100 1 $aKASPARY, T. E.
245 $aGenes related to flooding tolerance during germination and early growth of weedy rice.$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 10 June 2019/ Accepted: 6 July 2020.
520 $aAbstract: Evolution of flooding tolerance in weedy rice has occurred in several rice-growing regions, but the genes related to this process and the environmental effects are unknown. The objective of this study was to analyse the expression of genes related to flooding tolerance in response to temperature and flooding during the initial establishment of weedy rice. The experiments were carried out with rice cultivars IRGA 417 and Nipponbare, which are sensitive to flooding, and weedy rice ITJ03 and AV04 genotypes that have high and intermediate tolerance to flooding, respectively. The expression of genes related to reserve mobilisation, anaerobic respiration, escape and quiescence strategies was analysed at periods up to 24 days after sowing. The flooding tolerance of weedy rice genotype ITJ03 was associated with the expression of RAmy3D and OsTPP7, which are involved in the mobilisation of carbohydrate reserves, ADH1 and ADH2, which participate in anaerobic respiration, and SNRKL1 that triggers rapid elongation of the coleoptile and emergence. Although the genes PDC1,SUS3 and SUB1 are important for flooding tolerance in cultivated rice, their expression was not directly related to flooding tolerance in weedy rice. A temperature of 20°C reduced levels of expression of the RAmy3D, ADH2 and SNRKL1 genes and low temperature had a negative effect on the establishment of weedy rice. Breeding of rice genotypes with tolerance of low temperatures and anaerobic conditions may be a viable strategy to improve the control of weedy rice in paddy fields.
650 $aARROZ
650 $aMALEZAS
653 $aADAPTABILITY
653 $aESCAPE
653 $aHYPOXIA
653 $aINVASIVENESS
653 $aORYZA SATIVA L
653 $aQUIESCENCE
700 1 $aCUTTI, L.
700 1 $aSCHWALM RAFAELI, R.
700 1 $aANDREA DELATORRE, C.
700 1 $aMEROTTO JR, A.
773 $tWeed Research. 2020, vol. 60, p.435?449. DOI:https://doi.org/10.1111/wre.12448
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PÉREZ-RUCHEL, A.; BRITOS, A.; ALVARADO, A.; FERNÁNDEZ-CIGANDA, S.; GADEYNE, F.; BUSTOS, M.; ZUNINO, P.; CAJARVILLE, C. |
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Afiliación : |
ANALÍA PÉREZ-RUCHEL, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; ALEJANDRO BRITOS, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; AMAYRI ALVARADO, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; FREDERIK GADEYNE, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; MARIANA BUSTOS, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; CECILIA CAJARVILLE, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay. |
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Título : |
Impact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Animal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763 |
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ISSN : |
0377-8401. |
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DOI : |
10.1016/j.anifeedsci.2023.115763 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com -- |
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Contenido : |
This work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V. MenosThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearanc... Presentar Todo |
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Palabras claves : |
Gas production; Microbial protein synthesis; Microbiome profile; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Protein degradation. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 04021naa a2200301 a 4500
001 1064355
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0377-8401.
024 7 $a10.1016/j.anifeedsci.2023.115763$2DOI
100 1 $aPÉREZ-RUCHEL, A.
245 $aImpact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com --
520 $aThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V.
653 $aGas production
653 $aMicrobial protein synthesis
653 $aMicrobiome profile
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aProtein degradation
700 1 $aBRITOS, A.
700 1 $aALVARADO, A.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aGADEYNE, F.
700 1 $aBUSTOS, M.
700 1 $aZUNINO, P.
700 1 $aCAJARVILLE, C.
773 $tAnimal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763
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