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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha : |
07/06/2019 |
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Actualizado : |
18/11/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IRISARRI, P.; CARDOZO, G.; TARTAGLIA, C.; REYNO, R.; GUTIÉRREZ, P.; LATTANZI, F.; REBUFFO, M.; MONZA, J. |
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Afiliación : |
PILAR IRISARRI, Laboratorio de Microbiología, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA TARTAGLIA, Laboratorio de Bioquimica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, Uruguay.; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAMELA GUTIÉRREZ, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY. |
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Título : |
Selection of competitive and efficient rhizobia strains for white clover. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Frontiers in Microbiology, 23 April 2019, v. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768 |
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Páginas : |
11 p. |
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DOI : |
10.3389/fmicb.2019.00768 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds. |
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Contenido : |
The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains. MenosThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupan... Presentar Todo |
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Palabras claves : |
BIOLOGICAL NITROGEN FIXATION; INOCULANT; NATIVE-NATURALIZED RHIZOBIA STRAINS; NODULE OCCUPANCY; R. LEGUMINOSARUM SV. TRIFOLII; WHITE CLOVER. |
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Thesagro : |
FIJACION BIOLOGICA DEL NITRÓGENO; NODULACION; TREBOL BLANCO; TRIFOLIUM REPENS. |
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Asunto categoría : |
F60 Fisiología y bioquímica de la planta |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12797/1/Cardozo-Frontier-Micro-2019.pdf
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Marc : |
LEADER 03468naa a2200361 a 4500
001 1059873
005 2021-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2019.00768$2DOI
100 1 $aIRISARRI, P.
245 $aSelection of competitive and efficient rhizobia strains for white clover.$h[electronic resource]
260 $c2019
300 $a11 p.
500 $aArticle history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds.
520 $aThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains.
650 $aFIJACION BIOLOGICA DEL NITRÓGENO
650 $aNODULACION
650 $aTREBOL BLANCO
650 $aTRIFOLIUM REPENS
653 $aBIOLOGICAL NITROGEN FIXATION
653 $aINOCULANT
653 $aNATIVE-NATURALIZED RHIZOBIA STRAINS
653 $aNODULE OCCUPANCY
653 $aR. LEGUMINOSARUM SV. TRIFOLII
653 $aWHITE CLOVER
700 1 $aCARDOZO, G.
700 1 $aTARTAGLIA, C.
700 1 $aREYNO, R.
700 1 $aGUTIÉRREZ, P.
700 1 $aLATTANZI, F.
700 1 $aREBUFFO, M.
700 1 $aMONZA, J.
773 $tFrontiers in Microbiology, 23 April 2019$gv. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768
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INIA Tacuarembó (TBO) |
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| Registros recuperados : 71 | |
| 2. |  | CAZZULI, F. INIA Tacuarembó: Unidad Experimental Glencoe. ln: INIA Tacuarembó. Unidad Experimental Glencoe. Día de Campo Unidad Experimental Glencoe, 2012, [Colonia Fernando Baccaro, Guarapirú, Paysandú]. Propuestas tecnológicas para sistemas ganaderos de Basalto. Tacuarembó (Uruguay): INIA, 2012. p. 1-3 (INIA Serie Actividades de Difusión ; 693)| Biblioteca(s): INIA Tacuarembó. |
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| 7. | | MONTOSSI, F.; CAZZULI, F. Avances en la construcción de un modelo de intensificación sostenible de la ganadería del Uruguay. En: NÚÑEZ DOMÍNGUEZ, R.; RAMÍREZ VALVERDE, R.; FERNÁNDEZ RIVERA, S.; ARAÚJO FEBRES, O.; GARCÍA WINDER, M.; DÍAZ MUÑOZ, T. E. La ganadería en América Latina y el Caribe: alternativas para la producción competitiva, sustentable e incluyente de alimentos de origen animal. México: Biblioteca Básica de Agricultura, 2015. p. 607-622| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| 8. | | MONTOSSI, F.; CAZZULI, F. Beef marketing and quality in Uruguay. In: Simpósio de Produção de Gado de Corte (10o., Viçosa, Universidade Federal de Viçosa). Simpósio de Produção de Gado de Corte e Internacional Symposium of Beef Cattle Production, 6o. / Simpósio de Produção de Gado de Corte, 10., e Internacional Symposium of Beef Cattle Production, 6o., 26 a 28 de maio de 2016, Viçosa, MG ; editores Sebastião de Campos Valadares Filho … [et al.]. Anais. Viçosa, MG : UFV, DZO, 2016. 412 p. p. 41-66.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Tacuarembó. |
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| 10. | | MONTOSSI, F.; CAZZULI, F. From farm to fork: the uruguayan experience. In: Simpósio Internacional de Ciência da Carne (2o.Pirassununga, SP)Anais; Saulo da Luz e Silva et al. (Orgs). Pirassununga : Faculdade de Zootecnia e Engenharia de
Alimentos/Faculdade de Medicina Veterinária e Zootecnia, 2015. p. 22-33| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Tacuarembó. |
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| 11. | | MONTOSSI, F.; CAZZULI, F. Ejemplos empresariales y asociativos de marcas de calidad: Uruguay [Associative business examples and quality brands: Uruguay]. In: TEIXEIRA, A.; SAÑUDO, C. (Coord.). Marcas de calidad de carne en Iberoamerica: Caribe, Sudamerica y Peninsula Iberica. Congreso Iberoamericano de Marcas de Calidad de Carne y de Productos Cárnicos, 24-25 de octubre, Bragança, Portugal, 2019. Bragança (Portugal): RED MARCARNE, 2019. p. 181-190| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| 13. | | CAZZULI, F.; MONTOSSI, F. Introducción. In: MONTOSSI, F. (Ed.). Sistemas de suplementación mediante auto-suministro para la mejora de la recría invernal de terneros sobre campo natural en el norte del Uruguay. Montevideo (UY): INIA, 2017. p. 1-4 (INIA Serie Técnica; 234)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| 18. | | CAZZULI, F.; LAGOMARSINO, X.; MONTOSSI, F. Antecedentes. In: Montossi, F.; Cazzuli, F.; Lagomarsino, X. (Eds.). Sistemas de engorde de novillos sobre verdeos anuales estivales en la región de basalto. Montevideo (UY): INIA, 2017. p. 5-24. (INIA Serie Técnica; 230)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| 19. | | LAGOMARSINO, X.; CAZZULI, F.; MONTOSSI, F. Antecedentes. In: Montossi, F. (Ed.). Propuestas tecnológicas para el engorde de vacas de descarte en las regiones ganaderas de areniscas y basalto de Uruguay. Montevideo (UY): INIA, 2017. p. 9-34 (INIA Serie Técnica; 236) Anexos: p. 109-117.| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| Registros recuperados : 71 | |
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