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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/08/2018 |
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Actualizado : |
06/12/2018 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
ACUÑA, H.; INOSTROZA, L.; PINOCHET, D.; BALOCCHI, O.; ORTEGA, F.; ROMERO, O.; LÓPEZ, I.; SEGUEL, I.; TAPIA, G.; TORRES, A.; TEUBER, N.; GALDAMES, R.; REBUFFO, M. |
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Afiliación : |
H. ACUÑA, Facultad de Agronomía, Universidad de Concepción, Chile; L. INOSTROZA, CRI Quilamapu, Instituto de Investigaciones Agropecuarias INIA, Chile; D. PINOCHET, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Chile; O. BALOCCHI, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Chile; F. ORTEGA, CRI Carillanca, INIA, Chile; O. ROMERO, CRI Carillanca, INIA, Chile; I. LÓPEZ, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Chile; I. SEGUEL, CRI Carillanca, INIA, Chile; G. TAPIA, CRI Quilamapu, Instituto de Investigaciones Agropecuarias INIA, Chile; A. TORRES, CRI Remehue, INIA, Chile; N. TEUBER, Departamento Técnico, COVEPA SpA, Chile; R. GALDAMES, CRI Carillanca, INIA, Chile; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
White clover (Trifolium repens L.) germplasm selection for abiotic stress tolerance from naturalized populations collected in the southern regions of Chile . |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
In: Roldán-Ruiz I., Baert J., Reheul D. (eds). Breeding in a World of Scarcity. Springer, Cham (Chap.12). |
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Páginas : |
pp 81-87. |
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ISBN : |
978-3-319-28930-4 // Online ISBN 978-3-319-28932-8 |
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DOI : |
10.1007/978-3-319-28932-8_12 |
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Idioma : |
Inglés |
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Notas : |
Chapter history: First Online 14 June 2016. |
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Contenido : |
ABSTRACT.
Nine white clover populations (WCP) naturalized in Chile were evaluated under controlled (greenhouse pot experiments) and field conditions for water and P stress. The objective of this work was to determine dry matter production and water use and P efficiencies to identify which naturalized white clover populations would be most useful for breeding programs. In the
field experiments the clover was grown mixed with perennial ryegrass, but in pots the clover was grown alone. The treatments were the factorial combinations of WCP by water and P levels; completely randomized and complete randomized block experimental designs were used for pot and field experiments, respectively. The population WCP 9-1-X was the most
efficient for using water under controlled conditions and was among the three with the highest density of stolons under non-irrigated field conditions. The naturalized WCP 8-1-X produced more clover DM at the low P level than at the high P level and reached the same DM yield as cultivar Huia, which surpassed all the naturalized populations. These genetic
materials have high potential to be included in white clover breeding programs whose objective is to generate cultivars for water- restricted and/or P-defi cient marginal soils.
© Springer International Publishing Switzerland 2016 |
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Palabras claves : |
P EFFICIENCY; POT AND FIELD EXPERIMENTS; SOIL P STRESS; WATER STRESS; WATER USE EFFICIENCY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02465naa a2200361 a 4500
001 1058932
005 2018-12-06
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1007/978-3-319-28932-8_12$2DOI
100 1 $aACUÑA, H.
245 $aWhite clover (Trifolium repens L.) germplasm selection for abiotic stress tolerance from naturalized populations collected in the southern regions of Chile .$h[electronic resource]
260 $c2016
300 $app 81-87.
500 $aChapter history: First Online 14 June 2016.
520 $aABSTRACT. Nine white clover populations (WCP) naturalized in Chile were evaluated under controlled (greenhouse pot experiments) and field conditions for water and P stress. The objective of this work was to determine dry matter production and water use and P efficiencies to identify which naturalized white clover populations would be most useful for breeding programs. In the field experiments the clover was grown mixed with perennial ryegrass, but in pots the clover was grown alone. The treatments were the factorial combinations of WCP by water and P levels; completely randomized and complete randomized block experimental designs were used for pot and field experiments, respectively. The population WCP 9-1-X was the most efficient for using water under controlled conditions and was among the three with the highest density of stolons under non-irrigated field conditions. The naturalized WCP 8-1-X produced more clover DM at the low P level than at the high P level and reached the same DM yield as cultivar Huia, which surpassed all the naturalized populations. These genetic materials have high potential to be included in white clover breeding programs whose objective is to generate cultivars for water- restricted and/or P-defi cient marginal soils. © Springer International Publishing Switzerland 2016
653 $aP EFFICIENCY
653 $aPOT AND FIELD EXPERIMENTS
653 $aSOIL P STRESS
653 $aWATER STRESS
653 $aWATER USE EFFICIENCY
700 1 $aINOSTROZA, L.
700 1 $aPINOCHET, D.
700 1 $aBALOCCHI, O.
700 1 $aORTEGA, F.
700 1 $aROMERO, O.
700 1 $aLÓPEZ, I.
700 1 $aSEGUEL, I.
700 1 $aTAPIA, G.
700 1 $aTORRES, A.
700 1 $aTEUBER, N.
700 1 $aGALDAMES, R.
700 1 $aREBUFFO, M.
773 $tIn: Roldán-Ruiz I., Baert J., Reheul D. (eds). Breeding in a World of Scarcity. Springer, Cham (Chap.12).
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
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Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
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Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
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ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
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DOI : |
10.1111/imb.12875 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
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Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
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Marc : |
LEADER 02928naa a2200313 a 4500
001 1064356
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0962-1075 (print); 1365-2583 (electronic).
024 7 $a10.1111/imb.12875$2DOI
100 1 $aNOVAS, R.
245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. --
520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aPartnership for the goals - Goal 17
653 $aPest control
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aScrewworm
653 $aSustainable Development Goals (SDGs)
653 $aU6 promoter
700 1 $aBASIKA, T.
700 1 $aWILLIAMSON, M. E.
700 1 $aFRESIA, P.
700 1 $aMENCHACA, A.
700 1 $aSCOTT, M. J.
773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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