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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
05/12/2018 |
Actualizado : |
26/08/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
RUBIO, L.; HERNÁNDEZ, L.; BENÍTEZ, M. J.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R.; MAESO, D. |
Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA JOSÉ BENÍTEZ GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Journal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105. |
ISSN : |
1125-4653 // Online ISSN 2239-7264 |
DOI : |
10.1007/s42161-018-0149-0 |
Idioma : |
Inglés |
Notas : |
Article history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019.
Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República.
Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06). |
Contenido : |
ABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay.
© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018 MenosABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The su... Presentar Todo |
Palabras claves : |
BIOLOGICAL INDEXING; CITRUS TRISTEZA VIRUS (CTV); CLOSTEROVIRIDAE; CLOSTEROVIRUS; PHYLOGENETIC ANALYSIS. |
Thesagro : |
CITRUS. |
Asunto categoría : |
H20 Enfermedades de las plantas |
Marc : |
LEADER 03065naa a2200301 a 4500 001 1059343 005 2019-08-26 008 2019 bl uuuu u00u1 u #d 022 $a1125-4653 // Online ISSN 2239-7264 024 7 $a10.1007/s42161-018-0149-0$2DOI 100 1 $aRUBIO, L. 245 $aBiological and molecular characterization of Uruguayan citrus tristeza virus field isolates.$h[electronic resource] 260 $c2019 500 $aArticle history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019. Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República. Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06). 520 $aABSTRACT. Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018 650 $aCITRUS 653 $aBIOLOGICAL INDEXING 653 $aCITRUS TRISTEZA VIRUS (CTV) 653 $aCLOSTEROVIRIDAE 653 $aCLOSTEROVIRUS 653 $aPHYLOGENETIC ANALYSIS 700 1 $aHERNÁNDEZ, L. 700 1 $aBENÍTEZ, M. J. 700 1 $aARRUABARRENA, A. 700 1 $aRIVAS, F. 700 1 $aCOLINA, R. 700 1 $aMAESO, D. 773 $tJournal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
Fecha actual : |
12/01/2015 |
Actualizado : |
03/02/2018 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
DE BARBIERI, I.; NOLLA, M.; PLATERO, P.; LUZARDO, S.; VIÑOLES, C.; MONTOSSI, F. |
Afiliación : |
LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; M. NOLLA, Asesor privado; P. PLATERO, Asesor privado; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Evaluación de dos momentos de esquila preparto en majadas comerciales de la Región de basalto. |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
In: BERRETTA, E.; MONTOSSI, F.; BRITO, G. (Ed.). Alternativas tecnológicas para los sistemas ganaderos del basalto. Montevideo, UY: INIA, 2014. |
Páginas : |
267-272 |
Serie : |
(Serie Técnica; 217) |
ISSN : |
1688-9266 |
Idioma : |
Español |
Contenido : |
El objetivo del trabajo fue evaluar el impacto del momento de la esquila (mitad de gestación y un mes preparto) sobre variables
reproductivas y productivas en establecimientos comerciales de la región de Basalto, considerando los factores raza, edad y tipo
de parto. El trabajo se desarrolló en el marco del Proyecto INIA-BID Nº3; titulado Desarrollo tecnológico sustentable de la región
de Basalto y otras regiones de problemática similar. Este se ejecutó en forma conjunta con Central Lanera Uruguaya (CLU). |
Palabras claves : |
ESQUILA PREPARTO. |
Thesagro : |
BASALTO; ESQUILA; PREPARTO. |
Asunto categoría : |
-- L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3933/1/ST-217p267-272.pdf
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Marc : |
LEADER 01317naa a2200265 a 4500 001 1052003 005 2018-02-03 008 2014 bl uuuu u00u1 u #d 022 $a1688-9266 100 1 $aDE BARBIERI, I. 245 $aEvaluación de dos momentos de esquila preparto en majadas comerciales de la Región de basalto. 260 $c2014 300 $a267-272 490 $a(Serie Técnica; 217) 520 $aEl objetivo del trabajo fue evaluar el impacto del momento de la esquila (mitad de gestación y un mes preparto) sobre variables reproductivas y productivas en establecimientos comerciales de la región de Basalto, considerando los factores raza, edad y tipo de parto. El trabajo se desarrolló en el marco del Proyecto INIA-BID Nº3; titulado Desarrollo tecnológico sustentable de la región de Basalto y otras regiones de problemática similar. Este se ejecutó en forma conjunta con Central Lanera Uruguaya (CLU). 650 $aBASALTO 650 $aESQUILA 650 $aPREPARTO 653 $aESQUILA PREPARTO 700 1 $aNOLLA, M. 700 1 $aPLATERO, P. 700 1 $aLUZARDO, S. 700 1 $aVIÑOLES, C. 700 1 $aMONTOSSI, F. 773 $tIn: BERRETTA, E.; MONTOSSI, F.; BRITO, G. (Ed.). Alternativas tecnológicas para los sistemas ganaderos del basalto. Montevideo, UY: INIA, 2014.
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