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| Registros recuperados : 89 | |
| 61. |  | BLUMETTO, O.; CASTAGNA, A.; CARDOZO, G.; RUGGIA, A.; SCARLATO, S.; TISCORNIA, G.; GARCÍA, F.; AGUERRE, V. Desarrollando herramientas para evaluar el estado de los ecosistemas: El Índice de Integridad Ecosistémica (IIE). [Presentación oral y Resúmen] In: Libro de resúmenes de las TERCERAS JORNADAS INTERDISCIPLINARIAS EN BIODIVERSIDAD Y ECOLOGIA. "Desafíos socio-ambientales para el Uruguay del futuro" 28 de Noviembre a 2 de Diciembre 2016, Centro Universitario Regional del Este Rocha, Uruguay. p.104| Biblioteca(s): INIA Las Brujas. |
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| 66. | | CALVO, M.C.; DELGADO, S.; DUARTE, F.; GARCIA, F.; SCATONI, I.; GONZÁLEZ, A. Fruit flies (Diptera: Tephritidae) and hosts association in different ecosystems in Uruguay. International Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.369-380. doi: https://doi.org/10.1080/09670874.2022.2126023 Article history: Received 30 April 2022, Accepted 10 September 2022, Published online: 11 November 2022. Funding: The authors want to thank Emily Silva and Mity Schlesener from UFPel and Eugenia Lorenzo from the UDELAR for their support,...| Biblioteca(s): INIA Las Brujas. |
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| 71. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio y caracterización de una variante autóctona de Leptospira para su potencial uso como antígeno en vacunas de uso veterinario. In: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022. p.192-194. p.192-194.| Biblioteca(s): INIA Las Brujas. |
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| 72. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio de la capacidad infectiva, colonización renal e inmunogenicidad de Leptospira interrogans serogrupo Pomona serovar Kennewicki en un modelo murino sub-letal y crónico. [abstract]. Sub-área 4: Patogenia, virulencia y resistencia. In: Anais III Consensos Latinoamericanos em Leptospirose Animal. Nov 30-Dic 02, 2021 (evento online).| Biblioteca(s): INIA Las Brujas. |
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| 73. | | BARBAZÁN, M.; BAUTES, C.; BEUX, L.; BORDOLI, M.; CANO, J.D.; ERNST, O.; GARCIA-LAMOTHE, A.; GARCÍA, F.; QUINCKE, A. Fertilización potásica en cultivos de secano sin laboreo en Uruguay: rendimiento según análisis de suelos. [Fertilization with potassium in crops under no-tillage in Uruguay: yield response based on soil testing]. Agrociencia Uruguay, 2011, v.15, no.2, p.93-99| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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| 74. | | MOLINA, F.; TERRA, J.A.; OXLEY, A. M.; MARELLA, M.; CASTERÁ, F.; PLATERO, A.; GARCÍA, F.; ROVIRA, G.; ESCOSTEGUY, C. Indicadores tecnológicos-productivos zafra arrocera 2020-2021. In: Terra, J. A.; Martínez, S.; Saravia, H.; Mesones, B. (Eds.) Arroz 2021. Montevideo (UY): INIA, 2022. p. 1-5. (INIA Serie Técnica; 262)| Biblioteca(s): INIA Treinta y Tres. |
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| 76. | | CAPRA, G.; ALBIN, A.; RUGGIA, A.; GARCIA, F.; AGUERRE, V.; VILARO, F.; GILSANZ, J.C.; ARBOLEYA, J.; RACHETTI, M. Tecnología para la producción familiar del departamento de Rocha: avances del acuerdo de trabajo INIA-CNFR Revista INIA, 2009, no. 18, p. 40-44| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 78. | | BLUMETTO, O.; CASTAGNA, A.; CARDOZO, G.; GARCIA, F.; TISCORNIA, G.; RUGGIA, A.; SCARLATO, S.; ALBICETTE, M.M.; AGUERRE, V.; ALBÍN, A. Ecosystem integrity index, an innovative environmental evaluation tool for agricultural production systems. Ecological indicators, 2019, v. 101, p. 725-733. Article history: Received 4 July 2018; Received in revised form 9 January 2019; Accepted 29 January 2019.| Biblioteca(s): INIA Treinta y Tres. |
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| 80. | | MOLINA, F.; TERRA, J.A.; ROEL, A.; OXLEY, A.; MARELLA, M.; CASTERÁ, F.; PLATERO, A.; GARCÍA, F.; ROVIRA, G.; ESCOSTEGUI, C. Indicadores tecnológicos-productivos zafra arrocera 2020-2021. [Informe de zafra]. Treinta y Tres, (Uruguay): INIA, 2021, 18 p.| Biblioteca(s): INIA Treinta y Tres. |
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| Registros recuperados : 89 | |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
05/04/2022 |
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Actualizado : |
01/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
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Páginas : |
13 p. |
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DOI : |
10.3389/fpls.2022.804839 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
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Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
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Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
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Asunto categoría : |
H10 Plagas de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
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300 $a13 p.
500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario.
520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
653 $aDSRNA,
653 $aPEST CONTROL
653 $aRNA-SEQ
653 $aRNAI
653 $aSTINK BUG
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700 1 $aFRESIA, P.
700 1 $aMURCHIO, S.
700 1 $aMUJICA, V.
700 1 $aDALLA RIZZA, M.
773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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