| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
01/11/2021 |
|
Actualizado : |
01/11/2021 |
|
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
|
Autor : |
FERREIRA, V.; GONZÁLEZ-ARCOS, M.; PIANZZOLA, M.J.; COLL, N.S.; SIRI, M.I.; VALLS, M. |
|
Afiliación : |
VIRGINIA FERREIRA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JULIA PIANZZOLA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; NÚRIA S. COLL, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; MARÍA INÉS SIRI, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARC VALLS, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; Department of Genetics, University of Barcelona, Bellaterra, Catalonia, Spain. |
|
Título : |
Molecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato. |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
In: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18 |
|
Serie : |
eBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354). |
|
ISBN : |
978-1-0716-1608-6 (print) / 978-1-0716-1609-3 (e-book) |
|
ISSN : |
1064-3745 (print) / 1940-6029 (electronic) |
|
DOI : |
10.1007/978-1-0716-1609-3_18 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: First Online 27 August 2021. |
|
Contenido : |
ABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature. |
|
Palabras claves : |
Bacterial wilt; Disease resistance; Plant breeding; Potato brown rot; Ralstonia solanacearum; Solanum tuberosum. |
|
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
|
Marc : |
LEADER 02607naa a2200301 a 4500
001 1062509
005 2021-11-01
008 2021 bl uuuu u00u1 u #d
022 $a1064-3745 (print) / 1940-6029 (electronic)
024 7 $a10.1007/978-1-0716-1609-3_18$2DOI
100 1 $aFERREIRA, V.
245 $aMolecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato.$h[electronic resource]
260 $c2021
490 $aeBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354).
500 $aArticle history: First Online 27 August 2021.
520 $aABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
653 $aBacterial wilt
653 $aDisease resistance
653 $aPlant breeding
653 $aPotato brown rot
653 $aRalstonia solanacearum
653 $aSolanum tuberosum
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aPIANZZOLA, M.J.
700 1 $aCOLL, N.S.
700 1 $aSIRI, M.I.
700 1 $aVALLS, M.
773 $tIn: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 123 | |
| 6. |  | GONZÁLEZ-ARCOS, M.; CABOT, M. Evaluación de cultivares de tomate de mesa en invernadero para la región sur, ciclo primavera-verano, 2005-2006 ln: Reunión técnica. Resultados experimentales en tomate de mesa, 8 de junio de 2006, Las Brujas, Canelones, Uruguay Trabajos presentados. Las Brujas, Canelones (Uruguay): INIA, 2006. p.2-11 (INIA Serie Actividades de Difusión ; 454)| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
|    |
| 10. | | GONZÁLEZ-ARCOS, M.; GIMÉNEZ, G. (Ed.). Catálogo de cultivares hortícolas 2017 (2a.edición ampliada) Montevido (Uruguay): INIA, 2017. 56 p. (INIA Boletín de Divulgación; 113) Agradecimiento: Equipo de colaboradores del Proyecto Nacional de Mejoramiento Genético y Producción de Semilla en Horticultura: INIA Salto Grande (Ana Arruabarrena, Joanna Lado, Leticia Rubio, Pablo Varela, Brian Ghelfi, Johan Ghelfi,...| Tipo: Boletín de Divulgación |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
| |
| Registros recuperados : 123 | |
|
| Expresión de búsqueda válido. Check! |
|
|
