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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
07/11/2018 |
Actualizado : |
07/11/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASAL, A.; GARCÍA-ROCHE, M.; NAVAJAS, E.; CASSINA, A.; CARRIQUIRY, M. |
Afiliación : |
ALBERTO CASAL, Universidad de la República (UdelaR)/ Facultad de Agronomía; MERCEDES GARCÍA ROCHE, Universidad de la República (UdelaR)/ Facultad de Agronomía; Center for Free Radical and Biomedical Research (CEINBIO), Universidad de la República (UdelaR)/ Facultad de Medicina.; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIANA CASSINA, Center for Free Radical and Biomedical Research (CEINBIO), Universidad de la República (UdelaR)/ Facultad de Medicina.; MARIANA CARRIQUIRY, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
Título : |
Hepatic mitochondrial function in Hereford steers with divergent residual feed intake phenotypes. |
Complemento del título : |
Article. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Journal of Animal Science, 29 September 2018, volume 96, Issue 10, pages 4431-4443. |
ISSN : |
1525-3163 |
DOI : |
10.1093/jas/sky285 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 01 March 2018 // Accepted: 11 July 2018 // Published: 19 July 2018. |
Contenido : |
ABSTRACT.
Variations in phenotypic expression of feed efficiency could be associated with differences or inefficiencies in mitochondria function due to its impact on energy expenditure. The aim of this study was to determine hepatic mitochondrial density and function in terms of respiration, gene and protein expression, and enzyme activity of mitochondrial respiratory complex proteins, in steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111 and n = 122 for year 1 and 2, respectively) were evaluated in postweaning 70 d standard test for RFI. Forty-six steers exhibiting the greatest (n = 9 and 16 for year 1 and 2; high-RFI) and the lowest (n = 9 and 12 for year 1 and 2; low-RFI) RFI values were selected for this study. After the test, steers were managed together until slaughter under grazing conditions until they reached the slaughter body weight. At slaughter, hepatic samples (biopsies) were obtained. Tissue respiration was evaluated using high-resolution respirometry methods. Data were analyzed using a mixed model that included RFI group as fixed effect and slaughter date and year as a random effect using PROC MIXED of SAS. RFI and dry matter intake were different (P < 0.001) between low and high-RFI groups of year 1 and year 2. Basal respiration and maximum respiratory rate were greater (P ? 0.04) for low than high-RFI steers when complex II substrates (succinate) were supplied. However, when Complex I substrates (glutamate/malate) were used maximum respiratory capacity tended to be greater (P < 0.09) for low vs. high-RFI steers. Low-RFI steers presented greater mitochondria density markers (greater (P < 0.05) citrate synthase (CS) activity and tended (P ? 0.08) to have greater CS mRNA and mtDNA:nDNA ratio) than high-RFI steers. Hepatic expression SDHA, UQCRC1, and CYC1 mRNA was greater (P ? 0.02) and expression of NDUFA4, NDUFA13, SDHD, UQCRH, and ATP5E mRNA tended (P ? 0.10) to be greater in low than high-RFI steers. Hepatic SDHA protein expression tended (P < 0.08) to be greater while succinate dehydrogenase activity was greater (P = 0.04) and NADH dehydrogenase activity was greater (P = 0.03) for low than high-RFI steers. High-efficiency steers (low-RFI) probably had greater efficiency in hepatic nutrient metabolism, which was strongly associated with greater hepatic mitochondrial density and functioning, mainly of mitochondrial complex II.
© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.
For permissions, please e-mail: journals.permissions@oup.com. MenosABSTRACT.
Variations in phenotypic expression of feed efficiency could be associated with differences or inefficiencies in mitochondria function due to its impact on energy expenditure. The aim of this study was to determine hepatic mitochondrial density and function in terms of respiration, gene and protein expression, and enzyme activity of mitochondrial respiratory complex proteins, in steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111 and n = 122 for year 1 and 2, respectively) were evaluated in postweaning 70 d standard test for RFI. Forty-six steers exhibiting the greatest (n = 9 and 16 for year 1 and 2; high-RFI) and the lowest (n = 9 and 12 for year 1 and 2; low-RFI) RFI values were selected for this study. After the test, steers were managed together until slaughter under grazing conditions until they reached the slaughter body weight. At slaughter, hepatic samples (biopsies) were obtained. Tissue respiration was evaluated using high-resolution respirometry methods. Data were analyzed using a mixed model that included RFI group as fixed effect and slaughter date and year as a random effect using PROC MIXED of SAS. RFI and dry matter intake were different (P < 0.001) between low and high-RFI groups of year 1 and year 2. Basal respiration and maximum respiratory rate were greater (P ? 0.04) for low than high-RFI steers when complex II substrates (succinate) were supplied. However, when Complex I substrates (glutamate/malate) were used ... Presentar Todo |
Palabras claves : |
BEEF CATTLE; FEED EFFICIENCY; LIVER; MITOCHONDRIA; OXYGEN CONSUMPTION. |
Asunto categoría : |
L02 Alimentación animal |
Marc : |
LEADER 03483naa a2200265 a 4500 001 1059279 005 2018-11-07 008 2018 bl uuuu u00u1 u #d 022 $a1525-3163 024 7 $a10.1093/jas/sky285$2DOI 100 1 $aCASAL, A. 245 $aHepatic mitochondrial function in Hereford steers with divergent residual feed intake phenotypes.$h[electronic resource] 260 $c2018 500 $aArticle history: Received: 01 March 2018 // Accepted: 11 July 2018 // Published: 19 July 2018. 520 $aABSTRACT. Variations in phenotypic expression of feed efficiency could be associated with differences or inefficiencies in mitochondria function due to its impact on energy expenditure. The aim of this study was to determine hepatic mitochondrial density and function in terms of respiration, gene and protein expression, and enzyme activity of mitochondrial respiratory complex proteins, in steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111 and n = 122 for year 1 and 2, respectively) were evaluated in postweaning 70 d standard test for RFI. Forty-six steers exhibiting the greatest (n = 9 and 16 for year 1 and 2; high-RFI) and the lowest (n = 9 and 12 for year 1 and 2; low-RFI) RFI values were selected for this study. After the test, steers were managed together until slaughter under grazing conditions until they reached the slaughter body weight. At slaughter, hepatic samples (biopsies) were obtained. Tissue respiration was evaluated using high-resolution respirometry methods. Data were analyzed using a mixed model that included RFI group as fixed effect and slaughter date and year as a random effect using PROC MIXED of SAS. RFI and dry matter intake were different (P < 0.001) between low and high-RFI groups of year 1 and year 2. Basal respiration and maximum respiratory rate were greater (P ? 0.04) for low than high-RFI steers when complex II substrates (succinate) were supplied. However, when Complex I substrates (glutamate/malate) were used maximum respiratory capacity tended to be greater (P < 0.09) for low vs. high-RFI steers. Low-RFI steers presented greater mitochondria density markers (greater (P < 0.05) citrate synthase (CS) activity and tended (P ? 0.08) to have greater CS mRNA and mtDNA:nDNA ratio) than high-RFI steers. Hepatic expression SDHA, UQCRC1, and CYC1 mRNA was greater (P ? 0.02) and expression of NDUFA4, NDUFA13, SDHD, UQCRH, and ATP5E mRNA tended (P ? 0.10) to be greater in low than high-RFI steers. Hepatic SDHA protein expression tended (P < 0.08) to be greater while succinate dehydrogenase activity was greater (P = 0.04) and NADH dehydrogenase activity was greater (P = 0.03) for low than high-RFI steers. High-efficiency steers (low-RFI) probably had greater efficiency in hepatic nutrient metabolism, which was strongly associated with greater hepatic mitochondrial density and functioning, mainly of mitochondrial complex II. © The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. 653 $aBEEF CATTLE 653 $aFEED EFFICIENCY 653 $aLIVER 653 $aMITOCHONDRIA 653 $aOXYGEN CONSUMPTION 700 1 $aGARCÍA-ROCHE, M. 700 1 $aNAVAJAS, E. 700 1 $aCASSINA, A. 700 1 $aCARRIQUIRY, M. 773 $tJournal of Animal Science, 29 September 2018, volume 96, Issue 10, pages 4431-4443.
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Registros recuperados : 12 | |
1. | | GARCÍA ROCHE, M.; PEREZ, E. Aceites esenciales de plantas nativas promisorios para el control de bacteriosis en tomate. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Hortícola. Resultados experimentales en sanidad de tomate y morrón. Jornada de Divulgación. Canelones (Uruguay): INIA, 2015. p. 62-65 (Serie Actividades de Difusión ; 756). La investigación que da origen a los resultados presentados en la presente publicación recibió fondos de la Agencia Nacional de Investigación e Innovación bajo el código
INI_X_2013_1_10160 y del Instituto Nacional de Investigación...Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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4. | | CASAL, A.; GARCÍA-ROCHE, M.; NAVAJAS, E.; CASSINA, A.; CARRIQUIRY, M Differential hepatic oxidative status in steers with divergent residual feed intake phenotype. Animal, 1 January 2020, Volume 14, Issue 1, Pages 78-85. Doi: https://doi.org/10.1017/S1751731119001332 Article history: Received 12 December 2018 // Accepted 6 May 2019 // Published online: 20 June 2019.
Supplementary materials.
Corresponding author: Casal, A.; Departamento de Produccion Animal y Pasturas, Facultad de Agronomia,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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5. | | GARCIA-ROCHE, M.; CAÑIBE, G.; TALMÓN, D.; MENDOZA, A.; CASSINA, A.; QUIJANO, C.; CARRIQUIRY, M. Abundancia mitocondrial hepática en dos genotipos holstein en pastoreo. In: Jornadas Uruguayas de Buiatría, 48., 2021. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 31 de Mayo al 4 de Junio 2021. [Poster]. p.251-253.Biblioteca(s): INIA La Estanzuela. |
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8. | | GARCÍA-ROCHE, M.; TALMÓN, D.; CAÑIBE, G.; MENDOZA, A.; QUIJANO, C.; CASSINA, A.; CARRIQUIRY, M. Expresión génica hepática de la vía del receptor alfa activado por proliferador de peroxisomas A (PPARA) en dos genotipos Holstein. [abstract]. In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Cambio Climático y Producción Sostenible (Climate Change Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.2. (Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1)Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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9. | | GARCÍA-ROCHE, M.; TALMÓN, D.; CAÑIBE, G.; ASTESSIANO, A.L.; MENDOZA, A.; QUIJANO, C.; CASSINA, A.; CARRIQUIRY, M. Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system. Journal of Dairy Science, July 2022, Volume 105, Issue 7, Pages 5723-5737. OPEN ACCESS. doi: https://doi.org/10.3168/jds.2021-21358 Article history: Received 30 September 2021, Accepted 17 March 2022, Available online 20 May 2022, Version of Record 22 June 2022. -- Corresponding author: García-Roche, M.; Departamento de Producción Animal y Pasturas, Facultad de...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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10. | | GARCÍA-ROCHE, M.; TALMÓN, D.; CAÑIBE, G.; ASTESSIANO, A.L.; MENDOZA, A.; CASSINA, A.; QUIJANO, C.; CARRIQUIRY, M. Hepatic metabolism of grazing cows of two Holstein strains under two feeding strategies with different levels of pasture inclusion. PLoS ONE, October 2023, Volume 18, Issue 10, e0290551. https://doi.org/10.1371/journal.pone.0290551 -- OPEN ACCESS. Article history: Received March 22 2023; Accepted August 9 2023; Published October 26 2023. -- Correspondence: García-Roche, M.; Facultad de Agronomía, Departamento de Producción Animal y Pasturas, Universidad de la República,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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11. | | GARCIA ROCHE, M.; TALMÓN, D.; JORGE-SMEDING, E.; CASAL, A.; MENDOZA, A.; QUIJANO, C.; CARRIQUIRY, M; CASSINA, A. Función mitocondrial en biopsias de hígado de vacas lecheras de dos genotipos Holstein en condiciones pastoriles. [Resumen]. En: CONGRESO ASOCIACIÓN URUGUAYA DE PRODUCCIÓN ANIMAL (6º, Marzo, 2018, Tacuarembó, Uruguay). Tacuarembó: AUPA, 2018. p. 34.Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA La Estanzuela. |
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12. | | GARCIA-ROCHE, M.; CAÑIBE, G.; ASTESSIANO, A.; TALMON, D.; MENDOZA, A.; CASSINA, A.; QUIJANO, C.; CARRIQUIRY, M. O14 Hepatic energy reserves and carnitine palmitoyltransferase activity in grazing dairy cows of two pasture-based systems during lactation. [conference abstract] Animal - science proceedings, August 2022, Volume 13, Issue 3, pages 260-261. https://doi.org/10.1016/j.anscip.2022.07.024 Article histrory: Available online 16 September 2022, Version of Record 16 September 2022. -- Funding: MGR was supported by CAP fellowship BDDX_2018_1#49004502. The project was funded by CSIC I+D 2018 ID 103 awarded to MC and CQ and by...Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 12 | |
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