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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
07/10/2014 |
Actualizado : |
05/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ALARCÓN-REVERTE, R.; GARCIA, M.A.; WATSON, S.B.; ABDALLAH, I.; SABATÉ, S.; HERNÁNDEZ, M.J.; DAYAN ,F.E.; FISCHER, A.J. |
Afiliación : |
MILTON ALEJANDRO GARCIA LATASA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Pest Management Science, v. 71, n. 7, p. 996-2007, 2015. |
ISSN : |
1526-4998. |
DOI : |
10.1002/ps.3878 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 |
Contenido : |
Abstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different populations may carry different resistance mechanisms and different mechanisms can
act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant
expression of EPSPS sensitivity to glyphosate. MenosAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different popula... Presentar Todo |
Palabras claves : |
5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; EPSPS EXPRESSION; EPSPS INHIBITION ASSAY; POLYPLOIDY; RESISTANCE MECHANISM. |
Thesagro : |
RESISTENCIA A GLIFOSATO. |
Asunto categoría : |
H60 Malezas y escardas |
Marc : |
LEADER 02883naa a2200313 a 4500 001 1050961 005 2019-11-05 008 2015 bl uuuu u00u1 u #d 022 $a1526-4998. 024 7 $a10.1002/ps.3878$2DOI 100 1 $aALARCÓN-REVERTE, R. 245 $aConcerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California.$h[electronic resource] 260 $c2015 500 $aArticle history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 520 $aAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide, was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance. RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption, translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants. Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants. CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same population, individuals from different populations may carry different resistance mechanisms and different mechanisms can act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant expression of EPSPS sensitivity to glyphosate. 650 $aRESISTENCIA A GLIFOSATO 653 $a5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE 653 $aEPSPS EXPRESSION 653 $aEPSPS INHIBITION ASSAY 653 $aPOLYPLOIDY 653 $aRESISTANCE MECHANISM 700 1 $aGARCIA, M.A. 700 1 $aWATSON, S.B. 700 1 $aABDALLAH, I. 700 1 $aSABATÉ, S. 700 1 $aHERNÁNDEZ, M.J. 700 1 $aDAYAN ,F.E. 700 1 $aFISCHER, A.J. 773 $tPest Management Science$gv. 71, n. 7, p. 996-2007, 2015.
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6. | | GARCIA, M.A.; ALARCON-REVERTE, R.; DAYAN, F.E.; WATSON, S.; FISCHER, A. Mutations reduce EPSPS sensitivity to glyphosate in Echinochloa colona from California. In: WEED SCIENCE SOCIETY OF AMERICA; NORTHEASTERN WEED SCIENCE SOCIETY JOINT MEETING, 2013, Baltimore, MD, US. [Presentations] section 11. physiology; student poster contest: n. 113. Baltimore, MD, US: WSSA/NWSS, 2013.Biblioteca(s): INIA La Estanzuela. |
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8. | | CAMARGO, E. R.; ZAPIOLA, M.L.; AVILA, L.A.D; GARCIA, M.A.; PLAZA, G.; GAZZIERO, D.; VERONICA HOYOS, V. Current situation regarding herbicide regulation and public perception in South America(Conference Paper). Weed Science, 1 May 2020, Volume 68, Issue 3, Pages 232-239. Doi: https://doi.org/10.1017/wsc.2020.14 Article history: Received: 13 December 2019.//Revised: 27 January 2020//Accepted: 29 January 2020.Author for correspondence:Edinalvo Rabaioli Camargo, Department of Crop Protection, Federal University of Pelotas,Pelotas, RS, Brazil...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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9. | | DO CANTO, J.; KAVANOVÁ, M.; GARCÍA, M.A.; DE AVILA, A.; FRESIA, P.; TUESCA, D.H.; CONDON, F.; GAINES, T. Proyecto: Resistencia a glifosato en raigrás anual en Uruguay-entender, reducir y prevenir- [Poster]. In: Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; Simposio RedBio Argentina, 11., Montevideo 12-15 noviembre, 2019. Serie Técnica 253: Libro de Resúmenes.Biblioteca(s): INIA Tacuarembó. |
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10. | | ALARCÓN-REVERTE, R.; GARCIA, M.A.; WATSON, S.B.; ABDALLAH, I.; SABATÉ, S.; HERNÁNDEZ, M.J.; DAYAN ,F.E.; FISCHER, A.J. Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. Pest Management Science, v. 71, n. 7, p. 996-2007, 2015. Article history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
Biblioteca(s): INIA La Estanzuela. |
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11. | | DO CANTO, J.; KAVANOVÁ, M.; GARCÍA, M.A.; DE AVILA, L.A.; FRESIA, P.; TUESCA, D.H.; CONDON, F.; GAINES, T. Proyecto: Resistencia a glifosato en raigrás anual en Uruguay-entender, reducir y prevenir- [Resumen] In: REDBIO; INIA (Instituto Nacional de Investigación Agropecuaria); REDBIO Argentina. X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria y XI Simposio Redbio Argentina. Libro de Resúmenes. Montevideo 12 - 15 Noviembre 2019. Montevideo (UY): INIA, 2019. p. 76. (INIA Serie Técnica; 253)Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Tacuarembó. |
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Registros recuperados : 11 | |
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