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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
21/02/2014 |
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Actualizado : |
24/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GALETTA, M.A.; REINA, L.; RESQUÍN, F.; MANTERO, C.; GONZÁLEZ-PÉREZ, J.A.; ALMENDROS, G.; MENÉNDEZ, M.P.; GONZÁLEZ-VILADA, F.J. |
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Afiliación : |
Universidad de la República (UdelaR)/ Facultad de Química.; Universidad de la República (UdelaR)/ Facultad de Química.; JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República (UdelaR)/ Facultad de Agronomía.; Instituto de Recursos Naturales y Agrobiología de Sevilla, IRNAS-CSIC, Seville, Spaine.; SpaineMNCN-CSIC, Madrid, Spain.; Universidad de la República (UdelaR)/ Facultad de Química.; Instituto de Recursos Naturales y Agrobiología de Sevilla, IRNAS-CSIC, Seville, Spain. |
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Título : |
Chemometric appraisal of lignin pyrolytic assemblages from Eucalyptus woods relevant for pulping in Uruguay. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Analytical and Applied Pyrolysis, 2014, v. 109, p. 296-303. |
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DOI : |
10.1016/j.jaap.2014.08.001 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 5 January 2014 // Accepted 2 August 2014 // Available online 13 August 2014. |
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Contenido : |
Analytical pyrolysis (Py-GC/MS) at 500°C was applied to study wood composition of Eucalyptus species(Eucalyptus grandis, Eucalyptus dunnii and Eucalyptus benthamii) which are relevant for pulping inUruguay. Multivariate data treatments mainly principal component analysis and discriminant analy-sis were used to explore differences between the original wood cultivars. Multivariate analyses withautomatic backwards variable selection indicated that simplified methoxyphenol patterns (up to 10compounds) are sufficient for wood discrimination in terms of species and geographical origin butalso with purposes of forecasting the ease of delignification of the resulting pulps measured as activealkali. No additional chemotaxonomical accuracy was achieved when the data sets were enlarged withcarbohydrate-derived products. On the other side, discriminant or forecasting models (partial leastsquares and multiple linear regression) were much less significant when based on individual diag-nostic compounds, groups of compounds, or the classical syringyl-to-guaiacyl (S/G) ratio. Principalcomponent analysis indicated that the variability in lignin composition due to bioclimatic variations(spatial replications) was more significant than that due to phylogenetic differences (species andcultivars). |
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Palabras claves : |
ANALYTICAL PYROLYSIS; DISCRIMINANT ANALYSIS; FORESTACIÓN; LIGNIN METHOXYPHENOLS; MULTIPLE REGRESSION MODELS; PARTIAL LEAST SQUARES REGRESSION; PRINCIPAL COMPONENT ANALYSIS. |
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Asunto categoría : |
K10 Producción forestal |
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Marc : |
LEADER 02390naa a2200313 a 4500
001 1017467
005 2019-09-24
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jaap.2014.08.001$2DOI
100 1 $aGALETTA, M.A.
245 $aChemometric appraisal of lignin pyrolytic assemblages from Eucalyptus woods relevant for pulping in Uruguay.$h[electronic resource]
260 $c2014
500 $aArticle history: Received 5 January 2014 // Accepted 2 August 2014 // Available online 13 August 2014.
520 $aAnalytical pyrolysis (Py-GC/MS) at 500°C was applied to study wood composition of Eucalyptus species(Eucalyptus grandis, Eucalyptus dunnii and Eucalyptus benthamii) which are relevant for pulping inUruguay. Multivariate data treatments mainly principal component analysis and discriminant analy-sis were used to explore differences between the original wood cultivars. Multivariate analyses withautomatic backwards variable selection indicated that simplified methoxyphenol patterns (up to 10compounds) are sufficient for wood discrimination in terms of species and geographical origin butalso with purposes of forecasting the ease of delignification of the resulting pulps measured as activealkali. No additional chemotaxonomical accuracy was achieved when the data sets were enlarged withcarbohydrate-derived products. On the other side, discriminant or forecasting models (partial leastsquares and multiple linear regression) were much less significant when based on individual diag-nostic compounds, groups of compounds, or the classical syringyl-to-guaiacyl (S/G) ratio. Principalcomponent analysis indicated that the variability in lignin composition due to bioclimatic variations(spatial replications) was more significant than that due to phylogenetic differences (species andcultivars).
653 $aANALYTICAL PYROLYSIS
653 $aDISCRIMINANT ANALYSIS
653 $aFORESTACIÓN
653 $aLIGNIN METHOXYPHENOLS
653 $aMULTIPLE REGRESSION MODELS
653 $aPARTIAL LEAST SQUARES REGRESSION
653 $aPRINCIPAL COMPONENT ANALYSIS
700 1 $aREINA, L.
700 1 $aRESQUÍN, F.
700 1 $aMANTERO, C.
700 1 $aGONZÁLEZ-PÉREZ, J.A.
700 1 $aALMENDROS, G.
700 1 $aMENÉNDEZ, M.P.
700 1 $aGONZÁLEZ-VILADA, F.J.
773 $tJournal of Analytical and Applied Pyrolysis, 2014$gv. 109, p. 296-303.
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INIA Tacuarembó (TBO) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PÉREZ-RUCHEL, A.; BRITOS, A.; ALVARADO, A.; FERNÁNDEZ-CIGANDA, S.; GADEYNE, F.; BUSTOS, M.; ZUNINO, P.; CAJARVILLE, C. |
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Afiliación : |
ANALÍA PÉREZ-RUCHEL, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; ALEJANDRO BRITOS, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; AMAYRI ALVARADO, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; FREDERIK GADEYNE, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; MARIANA BUSTOS, Royal Agrifirm Group, Landgoedlaan 20, 7325 AW Apeldoorn, the Netherlands; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Av. Italia 3318, 11600 Montevideo, Uruguay; CECILIA CAJARVILLE, Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay. |
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Título : |
Impact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Animal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763 |
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ISSN : |
0377-8401. |
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DOI : |
10.1016/j.anifeedsci.2023.115763 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com -- |
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Contenido : |
This work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V. MenosThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearanc... Presentar Todo |
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Palabras claves : |
Gas production; Microbial protein synthesis; Microbiome profile; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Protein degradation. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 04021naa a2200301 a 4500
001 1064355
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0377-8401.
024 7 $a10.1016/j.anifeedsci.2023.115763$2DOI
100 1 $aPÉREZ-RUCHEL, A.
245 $aImpact of adding tannins or medium-chain fatty acids in a dairy cow diet on variables of in vitro fermentation using a rumen simulation technique (RUSITEC) system.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 15 August 2022, Revised 9 June 2023, Accepted 1 September 2023, Available online 6 September 2023, Version of Record 14 September 2023. -- Correspondence author: Pérez-Ruchel, A.; Departamento de Producción Animal y Salud de Sistemas Productivos (IPAV), Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.500, San José, Uruguay; email:anapevet@gmail.com --
520 $aThis work aimed to evaluate the effect of including 2 functional feed ingredients in a diet with a high proportion of pasture silage on in vitro ruminal fermentation, nutrient disappearances, and ruminal microbiomes. In a rumen simulation technique (RUSITEC) system (6 fermentation units), 4 treatments were evaluated: 1) CONTROL: a diet based on corn and grass silage, regular soybean meal (SBM), and corn; 178 g/kg of crude protein (CP) (used as negative control), 2) TSBM: Replacement of SBM by Mervobest® (used as positive control), 3) PHY (phytogenic mixture (tannins from quebracho and chestnut) at 10 g/kg dry matter (DM) replacing SBM) and 4) MCFA (mixture of medium-chain fatty acids, Aromabiotic Cattle® at 10 g/kg DM replacing SBM). Four runs were performed with 3 diets incubated simultaneously in duplicate using a balanced incomplete block design, lasting 17 days each. The inoculum was obtained from 3 rumen fistulated cows, and artificial saliva was infused at 650 mL/day. Samples were taken from each fermentation unit to determine the gas production, pH, NH3-N, and volatile fatty acids concentrations. The apparent digestibility of diet compounds was determined. Microbial protein synthesis and microbial community diversity were estimated using 15N as an external isotopic marker and by sequencing part of the 16 S rDNA gene, respectively. The TSBM and PHY treatments decreased NH3-N concentrations (P < 0.001) and the DM disappearance (P = 0.004). The crude protein disappearance was the lowest for TSBM followed by PHY (P < 0.001). The true protein disappearance was lower for TSBM and PHY than for CONTROL and MCFA (P < 0.001). The isovaleric acid proportion was lower for TSBM, MCFA, and PHY than for CONTROL. Treatments TSBM and MCFA registered lower neutral detergent fibre disappearance (P < 0.001) than the other diets but without changes in the quantified fibrolytic genera in the ruminal microbiota. The PHY treatment increased the relative abundance of Succinivibrio spp. and reduced that of Methanobrevibacter relative to that of CONTROL and TSBM, which could be linked to lower methanogenesis. The MCFA treatment increased Succinivibrio compared with TSBM and Succiniclasticum spp. compared with CONTROL. These results indicate that PHY inclusion at 10 g/kg DM decreased rumen protein degradation, similar to TSBM; while MCFA decreased rumen protein degradation to a lesser extent. Furthermore, both additives generated changes in the ruminal microbiota that would be associated with lower methane production. © 2023 Elsevier B.V.
653 $aGas production
653 $aMicrobial protein synthesis
653 $aMicrobiome profile
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aProtein degradation
700 1 $aBRITOS, A.
700 1 $aALVARADO, A.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aGADEYNE, F.
700 1 $aBUSTOS, M.
700 1 $aZUNINO, P.
700 1 $aCAJARVILLE, C.
773 $tAnimal Feed Science and Technology, November 2023, Volume 305, 115763. https://doi.org/10.1016/j.anifeedsci.2023.115763
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