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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
07/07/2021 |
Actualizado : |
27/06/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. |
Afiliación : |
NATALIA MANISSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; JUAN HERNÁN, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; MARIANA COSSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA E. FRANCIA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; TELMA GONZÁLEZ, Departamento de Parasitología y Micología, Facultad de Medicina, Instituto de Higiene, Universidad de La República, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; ANDRÉS IRIARTE, Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de La República Facultad de Medicina, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Pós-graduação em Ciência Animal nos Trópicos, Universidade Federal da Bahia, Salvador, Bahia, Brazil.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; SUSANA GONZÁLEZ, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay. |
Título : |
Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w |
DOI : |
10.1007/s11033-021-06492-w |
Idioma : |
Inglés |
Notas : |
Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). |
Contenido : |
Abstract:
Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. |
Palabras claves : |
Hammondia spp; NEOSPOROSIS; Neotropical canids; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Real-time PCR; TOXOPLASMOSIS. |
Asunto categoría : |
E16 Enfermedades de los animales |
Marc : |
LEADER 02795naa a2200349 a 4500 001 1062284 005 2022-06-27 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1007/s11033-021-06492-w$2DOI 100 1 $aMANISSE, N. 245 $aMolecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes$ba contribution to their molecular epidemiology.$h[electronic resource] 260 $c2021 500 $aArticle history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). 520 $aAbstract: Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. 653 $aHammondia spp 653 $aNEOSPOROSIS 653 $aNeotropical canids 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aReal-time PCR 653 $aTOXOPLASMOSIS 700 1 $aCABRERA, A. 700 1 $aHERNÁN, J. 700 1 $aCOSSE, M. 700 1 $aGIANNITTI, F. 700 1 $aFRANCIA, M.E. 700 1 $aGONZÁLEZ, T. 700 1 $aIRIARTE, A. 700 1 $aRIET-CORREA, F. 700 1 $aROBELLO, C. 700 1 $aGONZÁLEZ, S. 773 $tMolecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
20/07/2023 |
Actualizado : |
20/07/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
GONZÁLEZ, A.; HERNÁNDEZ, J.; DEL PINO, A.; HIRIGOYEN, A.; UBALDE, J. |
Afiliación : |
ALEJANDRO GONZÁLEZ, Soil and Water Department, College of Agronomy, University of the Republic, Garzón Avenue 780, Montevideo 12900, Uruguay; JORGE HERNÁNDEZ, Soil and Water Department, College of Agronomy, University of the Republic, Garzón Avenue 780, Montevideo 12900, Uruguay; AMABELIA DEL PINO, Soil and Water Department, College of Agronomy, University of the Republic, Garzón Avenue 780, Montevideo 12900, Uruguay; ANDRES EDUARDO HIRIGOYEN DOMINGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ UBALDE, Soil and Water Department, College of Agronomy, University of the Republic, Garzón Avenue 780, Montevideo 12900, Uruguay. |
Título : |
Harvest residue decomposition from Eucalyptus sp. plantations in temperate climate: indicators and contribution to nutrient cycling. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Forests. 2023, Volume 14, Issue 6, Article 1119. https://doi.org/10.3390/f14061119 --- OPEN ACCESS. |
ISSN : |
1999-4907 |
DOI : |
10.3390/f14061119 |
Idioma : |
Inglés |
Notas : |
Article history: Received 28 April 2023; Revised 17 May 2023; Accepted 26 May 2023; Published 28 May 2023. -- Academic Editors: SongzeWan, Jianping Wu and Shaofei Jin. -- Correspondence author: alejandrogonzalezuruguay@gmail.com . -- This article belongs to the Special Issue Soil Nutrient Cycling and Microbial Dynamics in Forests (https://www.mdpi.com/journal/forests/special_issues/4GVMPM2MQ5). -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( (https://creativecommons.org/licenses/by/4.0/). -- |
Contenido : |
The sustainable management of forest plantations by keeping the harvest residues on site improves the soil?s chemical, physical and biological properties while constituting an important nutrient reserve. Our objectives were: (a) to identify and quantify the characteristics of Eucalyptus dunnii, Eucalyptus grandis and Eucalyptus globulus that affect the decomposition rates of harvest residues, as well as indicators that can explain the process and (b) to quantify the potential recycling of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) to the soil from residue decomposition and the quantitative and qualitative differences between the species. We analyzed the information of five commercial plantations of Uruguay. At the harvest, the biomass of leaves, thin and thick branches, bark and their respective N, P, K, Ca and Mg contents were quantified. At each site, bags with samples of the different residues were left to decompose and were periodically collected throughout 24 months. Eucalyptus dunnii presented the largest amounts of residues of all parts. The decomposition rates of the different residues depended on their chemical constitution, part size and the species. Eucalyptus dunnii leaves showed the shortest half-life (0.94 years), while the bark of the same species presented the longest (5.62 years). Total nitrogen and carbon (total and soluble) contents, which can be easily determined, emerged as good predictors for half-life estimation. The release patterns of nutrients depended more on their dynamics in the plant and their parts than on the species itself. The results highlight the importance of nutrient recycling to ensure the sustainability of the productive system in the medium and long term. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosThe sustainable management of forest plantations by keeping the harvest residues on site improves the soil?s chemical, physical and biological properties while constituting an important nutrient reserve. Our objectives were: (a) to identify and quantify the characteristics of Eucalyptus dunnii, Eucalyptus grandis and Eucalyptus globulus that affect the decomposition rates of harvest residues, as well as indicators that can explain the process and (b) to quantify the potential recycling of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) to the soil from residue decomposition and the quantitative and qualitative differences between the species. We analyzed the information of five commercial plantations of Uruguay. At the harvest, the biomass of leaves, thin and thick branches, bark and their respective N, P, K, Ca and Mg contents were quantified. At each site, bags with samples of the different residues were left to decompose and were periodically collected throughout 24 months. Eucalyptus dunnii presented the largest amounts of residues of all parts. The decomposition rates of the different residues depended on their chemical constitution, part size and the species. Eucalyptus dunnii leaves showed the shortest half-life (0.94 years), while the bark of the same species presented the longest (5.62 years). Total nitrogen and carbon (total and soluble) contents, which can be easily determined, emerged as good predictors for half-life estimation. The r... Presentar Todo |
Palabras claves : |
Harvest residues half-life; Nutrient recycling; SISTEMA FORESTAL - INIA; Sustainability of the forest system; Uruguay. |
Asunto categoría : |
K01 Ciencias forestales - Aspectos generales |
URL : |
https://www.mdpi.com/1999-4907/14/6/1119/pdf
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Marc : |
LEADER 03320naa a2200265 a 4500 001 1064260 005 2023-07-20 008 2023 bl uuuu u00u1 u #d 022 $a1999-4907 024 7 $a10.3390/f14061119$2DOI 100 1 $aGONZÁLEZ, A. 245 $aHarvest residue decomposition from Eucalyptus sp. plantations in temperate climate$bindicators and contribution to nutrient cycling.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 28 April 2023; Revised 17 May 2023; Accepted 26 May 2023; Published 28 May 2023. -- Academic Editors: SongzeWan, Jianping Wu and Shaofei Jin. -- Correspondence author: alejandrogonzalezuruguay@gmail.com . -- This article belongs to the Special Issue Soil Nutrient Cycling and Microbial Dynamics in Forests (https://www.mdpi.com/journal/forests/special_issues/4GVMPM2MQ5). -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( (https://creativecommons.org/licenses/by/4.0/). -- 520 $aThe sustainable management of forest plantations by keeping the harvest residues on site improves the soil?s chemical, physical and biological properties while constituting an important nutrient reserve. Our objectives were: (a) to identify and quantify the characteristics of Eucalyptus dunnii, Eucalyptus grandis and Eucalyptus globulus that affect the decomposition rates of harvest residues, as well as indicators that can explain the process and (b) to quantify the potential recycling of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) to the soil from residue decomposition and the quantitative and qualitative differences between the species. We analyzed the information of five commercial plantations of Uruguay. At the harvest, the biomass of leaves, thin and thick branches, bark and their respective N, P, K, Ca and Mg contents were quantified. At each site, bags with samples of the different residues were left to decompose and were periodically collected throughout 24 months. Eucalyptus dunnii presented the largest amounts of residues of all parts. The decomposition rates of the different residues depended on their chemical constitution, part size and the species. Eucalyptus dunnii leaves showed the shortest half-life (0.94 years), while the bark of the same species presented the longest (5.62 years). Total nitrogen and carbon (total and soluble) contents, which can be easily determined, emerged as good predictors for half-life estimation. The release patterns of nutrients depended more on their dynamics in the plant and their parts than on the species itself. The results highlight the importance of nutrient recycling to ensure the sustainability of the productive system in the medium and long term. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. 653 $aHarvest residues half-life 653 $aNutrient recycling 653 $aSISTEMA FORESTAL - INIA 653 $aSustainability of the forest system 653 $aUruguay 700 1 $aHERNÁNDEZ, J. 700 1 $aDEL PINO, A. 700 1 $aHIRIGOYEN, A. 700 1 $aUBALDE, J. 773 $tForests. 2023, Volume 14, Issue 6, Article 1119. https://doi.org/10.3390/f14061119 --- OPEN ACCESS.
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