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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
23/10/2014 |
Actualizado : |
04/05/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
RESCONI, V.C.; CAMPO, M.M.; FONT I FURNOLS, M.; MONTOSSI, F.; SAÑUDO, C. |
Afiliación : |
V.C. RESCONI, Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain; M.M. CAMPO, Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain; FONT I FURNOLS, IRTA, España; FABIO MARCELO MONTOSSI PORCHILE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; C. SAÑUDO, Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, Zaragoza, Spain. |
Título : |
Sensory evaluation of castrated lambs finished on different proportions of pasture and concentrate feeding systems. |
Fecha de publicación : |
2009 |
Fuente / Imprenta : |
Meat Science, Volume 83, Issue 1, September 2009, Pages 31-37. https://doi.org/10.1016/j.meatsci.2009.03.004 |
DOI : |
10.1016/j.meatsci.2009.03.004 |
Idioma : |
Inglés |
Notas : |
Article history: Received 5 December 2008 // Received in revised form 16 March 2009 // Accepted 18 March 2009. |
Contenido : |
Castrated male Corriedale lambs from Uruguay were finished under one of four feeding systems, which differed in the level of pasture and the amount of concentrates. Treatment 1 (T1) was all pasture (P), T2 was P plus concentrate (C; 0.6% of live weight [LW]), T3 was P + C (1.2% of LW), and T4 was C plus alfalfa hay as a source of fibre (both ad libitum). A trained taste panel analysed samples from 96 lambs, using a quantitative descriptive method in a complete and balanced design. Eight of the 11 sensory attributes were affected by Treatment (p < 0.05). The inclusion of concentrate in the lamb diet improved the sensory quality of the meat, being related to its effect on lowering the intensity of undesirable odours and flavours (strange, rancid and acid), generating higher intensity of typical lamb aromas as well as producing higher tenderness. The frequencies of odour/flavour unsolicited observations also showed disadvantages to pasture feeding. Lambs fed only concentrates (T4) produced meat that had the highest fat flavour intensity and the best overall acceptability given by the panellists. |
Palabras claves : |
DIET; SHEEP MEAT; TASTE PANEL. |
Thesagro : |
OVINOS. |
Asunto categoría : |
L01 Ganadería |
Marc : |
LEADER 01953naa a2200241 a 4500 001 1051261 005 2020-05-04 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1016/j.meatsci.2009.03.004$2DOI 100 1 $aRESCONI, V.C. 245 $aSensory evaluation of castrated lambs finished on different proportions of pasture and concentrate feeding systems. 260 $c2009 500 $aArticle history: Received 5 December 2008 // Received in revised form 16 March 2009 // Accepted 18 March 2009. 520 $aCastrated male Corriedale lambs from Uruguay were finished under one of four feeding systems, which differed in the level of pasture and the amount of concentrates. Treatment 1 (T1) was all pasture (P), T2 was P plus concentrate (C; 0.6% of live weight [LW]), T3 was P + C (1.2% of LW), and T4 was C plus alfalfa hay as a source of fibre (both ad libitum). A trained taste panel analysed samples from 96 lambs, using a quantitative descriptive method in a complete and balanced design. Eight of the 11 sensory attributes were affected by Treatment (p < 0.05). The inclusion of concentrate in the lamb diet improved the sensory quality of the meat, being related to its effect on lowering the intensity of undesirable odours and flavours (strange, rancid and acid), generating higher intensity of typical lamb aromas as well as producing higher tenderness. The frequencies of odour/flavour unsolicited observations also showed disadvantages to pasture feeding. Lambs fed only concentrates (T4) produced meat that had the highest fat flavour intensity and the best overall acceptability given by the panellists. 650 $aOVINOS 653 $aDIET 653 $aSHEEP MEAT 653 $aTASTE PANEL 700 1 $aCAMPO, M.M. 700 1 $aFONT I FURNOLS, M. 700 1 $aMONTOSSI, F. 700 1 $aSAÑUDO, C. 773 $tMeat Science, Volume 83, Issue 1, September 2009, Pages 31-37. https://doi.org/10.1016/j.meatsci.2009.03.004
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Registro original : |
INIA Tacuarembó (TBO) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
17/08/2023 |
Actualizado : |
17/08/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. |
Afiliación : |
MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
Título : |
Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. |
ISSN : |
2076-2607 (electronic). |
DOI : |
10.3390/microorganisms11071704 |
Idioma : |
Inglés |
Notas : |
Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). |
Contenido : |
ABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalen... Presentar Todo |
Palabras claves : |
Dairy calf; MDR STEC; Mortality; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Shiga toxin-producing E. coli. |
Asunto categoría : |
L01 Ganadería |
URL : |
https://www.mdpi.com/2076-2607/11/7/1704/pdf
|
Marc : |
LEADER 04099naa a2200289 a 4500 001 1064289 005 2023-08-17 008 2023 bl uuuu u00u1 u #d 022 $a2076-2607 (electronic). 024 7 $a10.3390/microorganisms11071704$2DOI 100 1 $aFERNÁNDEZ, M. 245 $aShiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford, Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). 520 $aABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. 653 $aDairy calf 653 $aMDR STEC 653 $aMortality 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aShiga toxin-producing E. coli 700 1 $aCASAUX, M.L. 700 1 $aFRAGA, M. 700 1 $aVIGNOLI, R. 700 1 $aBADO, I. 700 1 $aZUNINO, P. 700 1 $aUMPIÉRREZ, A. 773 $tMicroorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS.
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