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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
17/08/2023 |
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Actualizado : |
17/08/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. |
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Afiliación : |
MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
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Título : |
Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. |
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ISSN : |
2076-2607 (electronic). |
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DOI : |
10.3390/microorganisms11071704 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). |
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Contenido : |
ABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalen... Presentar Todo |
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Palabras claves : |
Dairy calf; MDR STEC; Mortality; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Shiga toxin-producing E. coli. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.mdpi.com/2076-2607/11/7/1704/pdf
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Marc : |
LEADER 04099naa a2200289 a 4500
001 1064289
005 2023-08-17
008 2023 bl uuuu u00u1 u #d
022 $a2076-2607 (electronic).
024 7 $a10.3390/microorganisms11071704$2DOI
100 1 $aFERNÁNDEZ, M.
245 $aShiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford, Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221).
520 $aABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
653 $aDairy calf
653 $aMDR STEC
653 $aMortality
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aShiga toxin-producing E. coli
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aVIGNOLI, R.
700 1 $aBADO, I.
700 1 $aZUNINO, P.
700 1 $aUMPIÉRREZ, A.
773 $tMicroorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 28 | |
| 5. |  | BANCHERO, G.; FERNANDEZ, M.; GANZÁBAL, A.; VÁZQUEZ, A.; QUINTANS, G. Manejo genético y nutricional para aumentar la tasa mellicera de nuestras majadas. In: JORNADAS URUGUAYAS DE BUIATRÍA, 34., 2006, Paysandú, Uruguay. Paysandú, UY: Centro Médico Veterinario de Paysandú, SUB, SMVU. 2006. p. 71-76.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 12. | | IBÁÑEZ, M.; MANTERO, C.; RESQUÍN, F.; MONTORO, A.; PINTOS, X.; FERNÁNDEZ, M. Natural durability Eucalyptus bosistoana and Eucalyptus sideroxylon TIMBER. [Resumen de poster]. ln: Encuentro de Investigadores de la Región Noreste: Cerro Largo-Rivera-Tacuarembó, 1., 12 de agosto de 2016, Campus Interinstitucional de Tacuarembó, Tacuarembó. Libro de Resúmenes. Tacuarembó: UDELAR; INIA, 2016. p. 58| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Tacuarembó. |
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| 13. | | FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A....| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 15. | | RIVERO, R.; MINOLI, P.; PARODI, P.; MATTO, C.; ARMÚA-FERNÁNDEZ, M.T.; GIANNEECHINI, E.; CARVALHO, L.; VENZAL, J.M. Descripción de un foco de rangeliosis canina en el litoral noroeste del Uruguay. [Description of an outbreak of canine rangeliosis in the northwest littoral of Uruguay.]. Sección: Artículos originales. Veterinaria (Montevideo), 2017, vol. 54, no. 208, p. 15-22. -- OPEN ACCESS. Article history: Recibido 11 Octubre 2017, Aceptado 21 Noviembre 2017, Publicado 1 Diciembre 2017. -- Autor para correspondencia: rrivero@mgap.gub.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 16. | | PARODI, P.; ARMÚA-FERNÁNDEZ, M.T.; CORBELLINI, L.G.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M. Description of bovine babesiosis and anaplasmosis outbreaks in northern Uruguay between 2016 and 2018. Veterinary Parasitology: Regional Studies and Reports, 2022, Volume 29, Article number 100700. doi: https://doi.org/10.1016/j.vprsr.2022.100700 Article history: Received 7 April 2021; Received in revised form 27 November 2021; Accepted 26 January 2022; Available online 2 February 2022.
Corresponding author: Parodi, P.; Instituto Nacional de Investigación Agropecuaria,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 17. | | FÉLIX, M.L; ARMÚA-FERNÁNDEZ, M.T; PARODI, P.; BAZZANO , V.; MANGOLD, A.J.; VENZAL, J.M. Detection of a putative novel genotype of Anaplasma in gray-brocket deer (Mazama gouazoubira) from Uruguay. Experimental and Applied Acarology, Vol. 81, number 4, pag. 575-583, Aug 2020. Doi: https://doi.org/10.1007/s10493-020-00523-0 Article histort: Received 13 December 2019/Accepted03 July 2020/Published09 July 2020.We would like to thank Comisión Sectorial de Investigación Científica (CSIC), Programa Iniciación a la Investigación 2017, Project ID 160 for the...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 18. | | ANTÚNEZ, G.; CAJARVILLE, C.; FERNÁNDEZ, C.; DAYUTO, J.; ARTÚS, L; FERNÁNDEZ, M.; HORNOS, L.; CORREA, F.; BENTANCUR, O.; REPETTO, J. Estrategias de alimentación en la cría y recría de terneras lecheras y sus efectos sobre el crecimiento y la eficiencia de conservación. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 204-207.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 19. | | PARODI, P.; BAZZANO, V.; ARMÚA-FERNÁNDEZ, M.T.; FÉLIX, M.L.; CARVALHO, L.A.; FREIRE, J.; VENZAL, J.M. Molecular survey of Piroplasmida, Hepatozoon spp. and Anaplasmataceae in anemic and thrombocytopenic dogs from Uruguay. Original article. Veterinary Parasitology: Regional Studies and Reports. 2024, Volume 51, 101027. https://doi.org/10.1016/j.vprsr.2024.101027 Article history: Received 28 February 2024, Revised 8 April 2024, Accepted 17 April 2024, Available online 21 April 2024, Version of Record 23 April 2024. -- Corresponding author at: Rivera 1350, CP 50000 Salto, Uruguay. E-mail address:...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 20. | | UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 28 | |
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