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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/05/2023 |
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Actualizado : |
24/05/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
YANES, M.L.; FERNÁNDEZ, A.; ARIAS, A.; ALTIER, N. |
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Afiliación : |
M. L. YANES, Instituto de Investigaciones Biológica Clemente Estable, Laboratorio de Ecología Microbiana, Montevideo, Uruguay; ALFREDO JOSÉ FERNÁNDEZ TECHERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. ARIAS, Instituto de Investigaciones Biológica Clemente Estable, Laboratorio de Ecología Microbiana, Montevideo, Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Method to evaluate disease supression against Pythium debaryanum and alfalfa growth promotion by fluorescent Pseudomonas. [Método para evaluar protección contra Pythium debaryanum y promoción del crecimiento de alfalfa por Pseudomonas fluorescentes]. [Special Issue 25 Years Agrociencia]. |
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Complemento del título : |
Plant Biology. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2022, vol. 26, NE2, e1087. doi: https://doi.org/10.31285/AGRO.26.1087 -- OPEN ACCESS. |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.26.1087 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article originally published in: Agrociencia (Uruguay). 2004;8(2):23-31. doi: https://doi.org/10.31285/AGRO.08.1002 -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/ ) |
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Contenido : |
ABSTRACT.- Fluorescent Pseudomonas have been extensively reported as effective biocontrol agents and can be used to manage Pythium seedling diseases in alfalfa. An in vivo methodology was developed to evaluate fluorescent Pseudomonas isolates for their ability to suppress disease and promote plant growth in the alfalfa-Pythium pathosystem. The standard method consists of a bioassay performed in growth chamber under controlled conditions of temperature and photoperiod. Alfalfa seeds were sown in commercial substrate and subsequently treated as follows: no Pseudomonas, no Pythium debaryanum (germination control); inoculated only with P. debaryanum (disease control); each one of the Pseudomonas isolates co-inoculated with P. debaryanum. An augmented experiment design was obtained by selecting a random complete blocks design for four checks systematically evaluated on 12 bioassays, and enlarging the blocks to accommodate up to 16 treatments per assay. Disease control recorded an emergence of 33.2% on day 15 after sowing date, as compared with germination control (100%). The 101 fluorescent Pseudomonas isolates showed a wide response on disease suppression ability. Twelve percent of tested isolates reasonably protected alfalfa plants against P. debaryanum, recording an emergence of over 60%. A similar procedure, in the absence of the pathogen, was used to evaluate alfalfa growth-promoting ability of selected Pseudomonas isolates. Five isolates exhibited a significant biocontrol activity against P. debaryanum and four of them also recorded a significant plant growth-promoting effect. Both bioassays were appropriate to identify candidate Pseudomonas isolates to be further tested under field conditions. Copyright (c) 2022 Agrociencia Uruguay MenosABSTRACT.- Fluorescent Pseudomonas have been extensively reported as effective biocontrol agents and can be used to manage Pythium seedling diseases in alfalfa. An in vivo methodology was developed to evaluate fluorescent Pseudomonas isolates for their ability to suppress disease and promote plant growth in the alfalfa-Pythium pathosystem. The standard method consists of a bioassay performed in growth chamber under controlled conditions of temperature and photoperiod. Alfalfa seeds were sown in commercial substrate and subsequently treated as follows: no Pseudomonas, no Pythium debaryanum (germination control); inoculated only with P. debaryanum (disease control); each one of the Pseudomonas isolates co-inoculated with P. debaryanum. An augmented experiment design was obtained by selecting a random complete blocks design for four checks systematically evaluated on 12 bioassays, and enlarging the blocks to accommodate up to 16 treatments per assay. Disease control recorded an emergence of 33.2% on day 15 after sowing date, as compared with germination control (100%). The 101 fluorescent Pseudomonas isolates showed a wide response on disease suppression ability. Twelve percent of tested isolates reasonably protected alfalfa plants against P. debaryanum, recording an emergence of over 60%. A similar procedure, in the absence of the pathogen, was used to evaluate alfalfa growth-promoting ability of selected Pseudomonas isolates. Five isolates exhibited a significant biocontrol a... Presentar Todo |
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Palabras claves : |
Biocontrol; PGPR; Promoción del crecimiento vegetal; Pythium. |
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Thesagro : |
CONTROL BIOLÓGICO; MEDICAGO SATIVA L; PSEUDOMONAS. |
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Asunto categoría : |
H01 Protección de plantas - Aspectos generales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17168/1/2730-5066-1087.pdf
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Marc : |
LEADER 03112naa a2200277 a 4500
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022 $a2730-5066
024 7 $a10.31285/AGRO.26.1087$2DOI
100 1 $aYANES, M.L.
245 $aMethod to evaluate disease supression against Pythium debaryanum and alfalfa growth promotion by fluorescent Pseudomonas. [Método para evaluar protección contra Pythium debaryanum y promoción del crecimiento de alfalfa por Pseudomonas fluorescentes]. [Special Issue 25 Years Agrociencia].$h[electronic resource]
260 $c2022
500 $aArticle history: Article originally published in: Agrociencia (Uruguay). 2004;8(2):23-31. doi: https://doi.org/10.31285/AGRO.08.1002 -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/ )
520 $aABSTRACT.- Fluorescent Pseudomonas have been extensively reported as effective biocontrol agents and can be used to manage Pythium seedling diseases in alfalfa. An in vivo methodology was developed to evaluate fluorescent Pseudomonas isolates for their ability to suppress disease and promote plant growth in the alfalfa-Pythium pathosystem. The standard method consists of a bioassay performed in growth chamber under controlled conditions of temperature and photoperiod. Alfalfa seeds were sown in commercial substrate and subsequently treated as follows: no Pseudomonas, no Pythium debaryanum (germination control); inoculated only with P. debaryanum (disease control); each one of the Pseudomonas isolates co-inoculated with P. debaryanum. An augmented experiment design was obtained by selecting a random complete blocks design for four checks systematically evaluated on 12 bioassays, and enlarging the blocks to accommodate up to 16 treatments per assay. Disease control recorded an emergence of 33.2% on day 15 after sowing date, as compared with germination control (100%). The 101 fluorescent Pseudomonas isolates showed a wide response on disease suppression ability. Twelve percent of tested isolates reasonably protected alfalfa plants against P. debaryanum, recording an emergence of over 60%. A similar procedure, in the absence of the pathogen, was used to evaluate alfalfa growth-promoting ability of selected Pseudomonas isolates. Five isolates exhibited a significant biocontrol activity against P. debaryanum and four of them also recorded a significant plant growth-promoting effect. Both bioassays were appropriate to identify candidate Pseudomonas isolates to be further tested under field conditions. Copyright (c) 2022 Agrociencia Uruguay
650 $aCONTROL BIOLÓGICO
650 $aMEDICAGO SATIVA L
650 $aPSEUDOMONAS
653 $aBiocontrol
653 $aPGPR
653 $aPromoción del crecimiento vegetal
653 $aPythium
700 1 $aFERNÁNDEZ, A.
700 1 $aARIAS, A.
700 1 $aALTIER, N.
773 $tAgrociencia Uruguay, 2022, vol. 26, NE2, e1087. doi: https://doi.org/10.31285/AGRO.26.1087 -- OPEN ACCESS.
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INIA Las Brujas (LB) |
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| Registros recuperados : 6 | |
| 1. |  | CARMO, P.M.S.D.; UZAL , F.A.; PEDROSO, P.M.O.; RIET-CORREA, F. Conidiobolomycosis, cryptococcosis, and aspergillosis in sheep and goats: a review. Journal of Veterinary Diagnostic Investigation, Vol. 32, Issue 6, pag.826-834, Nov. 2020. Doi: https://doi.org/10.1177/1040638720958338 Article history: First Published September 14, 2020.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 2. | | MACHADO, M.; RIET-CORREA, F.; PEDROSO, P.M.O.; MACÊDO, J.T.S.A. Doramectin intoxication in malnourished 15-month-old cattle. [Intoxicação por doramectina em bovinos malnutridos de 15 meses de idade.] Pesquisa Veterinaria Brasileira, 2022, Volume 42, Article e07102. OPEN ACCESS. doi: https://doi.org/10.1590/1678-5150-PVB-7102 Article history: Received 15 May 2022; Accepted 31 May 2022; Publication 29 July 2022; Date of issue 2022.
Corresponding author: Riet-Correa, F.; Animal Sciences in the Tropics (PPGCAT), Faculdade de Medicina Veterinária e Zootecnia,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 3. | | MACÊDO, J.T.S.A.; BISCARDE, C.E; PESCADOR, C.A.; NAKAZATO, L.; FONSECA, N.D.-S.; UBIALI, D.G; RIET-CORREA, F.; PEDROSO, P.M.O. Conidiobolomycosis in goats. [Conidiobolomicose em caprinos]. Pesquisa Veterinaria Brasileira, 2021, Volume 41, Article number e06978. doi: https://doi.org/10.1590/1678-5150-PVB-6978 Article history: Received on June 17, 2021/Accepted for publication on September 23, 2021.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 4. | | PEDROSO, P. M. O.; SANTOS, M. V. B.; SEUS, V. G.; SILVA, R. M. M. DA; RIET-CORREA, F.; MACÊDO, J. T. S. A. Intoxicação por metternichia princeps (Solanaceae) em caprinos no Estado da Bahia. Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 448-450, maio 2015.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 2 |
| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | SILVA, V. C.; OLIVEIRA, L. A. DE; LACERDA, M. DOS S. C.; PIMENTEL, L. A.; SANTOS, W. S.; MACÊDO, J. T. S. A. E; RIET-CORREA, F.; PEDROSO P. M. O. Experimental poisoning by cassava wastewater in sheep. (Intoxicação experimental por manipueira em ovinos). Pesquisa Veterinária Brasileira, Rio de Janeiro v. 37, n. 11, p. 1241-1246, novembro. 2017. Article history: Received: November 23, 2016// Accepted: March 17, 2017.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 6. | | MARCELINO, S.A.C; MACÊDO, J.T.S.A.; DOS REIS, S.D.S.; LACERDA, M.S.C.; DA SILVA, A.R.S; RIET-CORREA, F.; PIMENTEL, L.A.; PEDROSO, P.M.O. Malformations in small ruminants in the semi-arid region of Bahia: Epidemiological, clinical-pathological and radiological aspects. [Malformações em pequenos ruminantes no semiárido da Bahia: Aspectos epidemiológicos, clínico-patológicos e radiológicos]. Pesquisa Veterinaria Brasileira, 1 December 2017, Volume 37, Issue 12, Pages 1437-1442. OPEN ACCESS. Article history: Recebido em 3 de junho de 2017/Aceito para publicação em 15 de julho de 2017.| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 6 | |
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