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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
06/10/2022 |
Actualizado : |
06/10/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HANNAH BULLOCK, Synergy America Inc., Atlanta, GA 30329, USA; MARINA MAURENTE BERON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; NÉLIDA RODRÍGUEZ-OSORIO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; LUCIANA SILVA-FLANNERY, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS CABRERA, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay; RODRIGO PUENTES, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; JANA M. RITTER, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay. |
Título : |
Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 |
ISSN : |
1999-4915 |
DOI : |
10.3390/v14092042 |
Idioma : |
Inglés |
Notas : |
Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com --
Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock |
Contenido : |
ABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. MenosABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus mor... Presentar Todo |
Palabras claves : |
Abortion; Cattle; Emerging diseases; Epsilonpolyomavirus bovis; Nephropathy; Next generation sequencing; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Polyomavirus; Reproductive diseases; Viral diseases. |
Asunto categoría : |
L20 Ecología animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16810/1/viruses-14-02042-v2.pdf
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Marc : |
LEADER 04533naa a2200445 a 4500 001 1063641 005 2022-10-06 008 2022 bl uuuu u00u1 u #d 022 $a1999-4915 024 7 $a10.3390/v14092042$2DOI 100 1 $aGIANNITTI, F. 245 $aBovine Polyomavirus-1 (Epsilonpolyomavirus bovis)$bAn emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022. Academic Editors: Fernando Bauermann and Mayara Maggioli. Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com -- Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock 520 $aABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. 653 $aAbortion 653 $aCattle 653 $aEmerging diseases 653 $aEpsilonpolyomavirus bovis 653 $aNephropathy 653 $aNext generation sequencing 653 $aPathology 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPolyomavirus 653 $aReproductive diseases 653 $aViral diseases 700 1 $aSILVEIRA, C.S. 700 1 $aBULLOCK, H. 700 1 $aBERON, M. 700 1 $aFERNÁNDEZ-CIGANDA, S. 700 1 $aBENÍTEZ-GALEANO, M.J. 700 1 $aRODRÍGUEZ-OSORIO, N. 700 1 $aSILVA-FLANNERY, L. 700 1 $aPERDOMO, T. 700 1 $aCABRERA, A. 700 1 $aPUENTES, R. 700 1 $aCOLINA, R. 700 1 $aRITTER, J.M. 700 1 $aCASTELLS, M. 773 $tViruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042
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INIA Las Brujas (LB) |
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Registros recuperados : 4 | |
1. | | JÁUREGUI ,J.M.; MICHELINI ,D.F.; BAUDRACCO, J.; BAUDRACCO, J.; LATTANZI, F. Sistemas radicales más grandes no incrementan la supervivencia estival de macollos de Festuca Alta. [Resumen]. En: CONGRESO ASOCIACIÓN URUGUAYA DE PRODUCCIÓN ANIMAL (6º, Marzo, 2018, Tacuarembó, Uruguay). Tacuarembó: AUPA, 2018. p. 68.Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA La Estanzuela. |
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4. | | JÁUREGUI, J.M.; MICHELINI, D.F.; SEVILLA, G.H.; BERHONGARAY, G.; BERONE, G.D.; BAUDRACCO, J.; CHILIBROSTE, P.; AGNUSDEI, M.G.; LATTANZI, F. Tall fescue tiller survival over summer in a subtropical environment: The role of the size and depth of root systems. Journal of Agronomy and Crop Science. 2024, Volume 210, Issue 1, article e12682. https://doi.org/10.1111/jac.12682 Article history: Received 5 May 2023; Revised 20 October 2023; Accepted 1 November 2023. -- Correspondence: J. M. Jáuregui, Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Argentina. Email:...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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