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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/07/2018 |
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Actualizado : |
22/07/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERNÁNDEZ, S.; FRAGA, M.; SILVEYRA, E.; TROMBERT, A.N.; RABAZA, A.; PLA, M. |
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Afiliación : |
S. FERNÁNDEZ, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. SILVEYRA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); A.N. TROMBERT, Universidad Mayor, Santiago (Chile); A. RABAZA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARCELO PLA TEJERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Probiotic properties of native Lactobacillus spp. strains for dairy calves. |
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Complemento del título : |
Research article. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Beneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624. |
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ISSN : |
1876-2883 |
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DOI : |
10.3920/BM2017.0131 |
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Idioma : |
Inglés |
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Notas : |
Artilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018. |
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Contenido : |
ABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.
© 2018 Wageningen Academic Publishers. MenosABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten d... Presentar Todo |
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Palabras claves : |
GASTROINTESTINAL TRACT; LACTOBACILLUS SPP; NATIVE; NEONATAL CALF DIARRHOEA; PROBIOTIC POTENTIAL. |
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Thesagro : |
GANADO DE LECHE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02595naa a2200289 a 4500
001 1058830
005 2018-07-22
008 2018 bl uuuu u00u1 u #d
022 $a1876-2883
024 7 $a10.3920/BM2017.0131$2DOI
100 1 $aFERNÁNDEZ, S.
245 $aProbiotic properties of native Lactobacillus spp. strains for dairy calves.$h[electronic resource]
260 $c2018
500 $aArtilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018.
520 $aABSTRACT. The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves. © 2018 Wageningen Academic Publishers.
650 $aGANADO DE LECHE
653 $aGASTROINTESTINAL TRACT
653 $aLACTOBACILLUS SPP
653 $aNATIVE
653 $aNEONATAL CALF DIARRHOEA
653 $aPROBIOTIC POTENTIAL
700 1 $aFRAGA, M.
700 1 $aSILVEYRA, E.
700 1 $aTROMBERT, A.N.
700 1 $aRABAZA, A.
700 1 $aPLA, M.
773 $tBeneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624.
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INIA Las Brujas (LB) |
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| Registros recuperados : 40 | |
| 8. |  | FERNÁNDEZ ,S.; FRAGA, M.; CASTELLS, M.; COLINA, R.; ZUNINO,P. Effect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves. Beneficial Microbes, 1 September 2020, Volume 11, Issue 5, Pages 477-488. Doi: https://doi.org/10.3920/BM2019.0167| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 13. | | QUERO, C.; FERNANDEZ, S.; BRANDARIZ, S.B.; SIMONDI, S.; GUTIÉRREZ, L. Herramientas de análisis y visualización genómica. MV 10 - COMUNICACIONES LIBRES - MV. MEJORAMIENTO VEGETAL In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 285| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
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| 16. | | QUERO, G.; BONNECARRERE, V.; FERNÁNDEZ, S.; SILVA, P.; SIMONDI, S.; BORSANI, O. La eficiencia del uso de luz y la partición energética en arroz es genotipo dependiente. [resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, XI. Encuentro Nacional de REDBIO, III. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2018. p.8-9 (Serie Actividades de Difusión; 786)| Biblioteca(s): INIA Las Brujas. |
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| 17. | | QUERO, G.; SIMONDI, S.; CERETTA, S.; OTERO, A.; GARAYCOCHEA, S.; FERNANDEZ, S.; BORSANI, O.; BONNECARRERE, V. An integrative analysis of yield stability for a GWAS in a small soybean breeding population. Crop Science, May 2021, volume 61, issue 3, pages 19003-1914. Doi: https://doi.org/10.1002/csc2.20490 Article history: Received, 3 November 2020; Accepted, 11 February 2021; Published online, 14 April 2021.
Associate Editor: Junping Chen.
The authors thank Edgardo Rey and Wanda Iriarte for technical assistance in field experiment and...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 20. | | MEDEROS, A.; CARRACELAS, B.; MINHO, A.P.; FERNÁNDEZ, S.; SÁNCHEZ, J. Prevalence and Factors Associated with Anthelmintic Resistance in Gastrointestinal Nematodes of Cattle: A Systematic Review and Meta-analysis. Journal of Veterinary Medicine and Health, v. 2, no. 2, 2018. OPEN ACCESS. Article history: Received November 21, 2018; Accepted December 02, 2018; Published December 17, 2018.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| Registros recuperados : 40 | |
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