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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/09/2022 |
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Actualizado : |
22/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. |
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Afiliación : |
ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; DÉBORAH ERNST, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ANDREA CARDOZO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ALEXIA TORRES, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ROBERTO VIDAL, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile; Instituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 |
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Descripción física : |
SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) |
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ISSN : |
0719-8132 (print); e-ISSN 0719-8000 (electronic) |
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DOI : |
10.4067/S0719-81322022000200045 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy |
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Contenido : |
ABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. MenosABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The ... Presentar Todo |
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Palabras claves : |
Antimicrobial resistance; Non-O157 STEC; PLATAFORMA EN SALUD ANIMAL; Shiga toxin subtypes. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16768/1/10.4067-s0719-81322022000200045.pdf
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Marc : |
LEADER 03084naa a2200325 a 4500
001 1063578
005 2022-09-22
008 2022 bl uuuu u00u1 u #d
022 $a0719-8132 (print); e-ISSN 0719-8000 (electronic)
024 7 $a10.4067/S0719-81322022000200045$2DOI
100 1 $aUMPIÉRREZ , A.
245 $aNon-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay.$h[electronic resource]
260 $c2022
300 $cSSN 0719-8132 (version on-line) ISSN 0719-8000 (version print)
500 $aArticle history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022. Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy
520 $aABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics.
653 $aAntimicrobial resistance
653 $aNon-O157 STEC
653 $aPLATAFORMA EN SALUD ANIMAL
653 $aShiga toxin subtypes
700 1 $aERNST, E.
700 1 $aCARDOZO, A.
700 1 $aTORRES, A.
700 1 $aFERNÁNDEZ, M.
700 1 $aFRAGA, M.
700 1 $aVIGNOLI, R.
700 1 $aBADO, I.
700 1 $aVIDAL, R.
700 1 $aZUNINO, P.
773 $tAustral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045
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INIA Las Brujas (LB) |
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| Registros recuperados : 28 | |
| 5. |  | BANCHERO, G.; FERNANDEZ, M.; GANZÁBAL, A.; VÁZQUEZ, A.; QUINTANS, G. Manejo genético y nutricional para aumentar la tasa mellicera de nuestras majadas. In: JORNADAS URUGUAYAS DE BUIATRÍA, 34., 2006, Paysandú, Uruguay. Paysandú, UY: Centro Médico Veterinario de Paysandú, SUB, SMVU. 2006. p. 71-76.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 12. | | IBÁÑEZ, M.; MANTERO, C.; RESQUÍN, F.; MONTORO, A.; PINTOS, X.; FERNÁNDEZ, M. Natural durability Eucalyptus bosistoana and Eucalyptus sideroxylon TIMBER. [Resumen de poster]. ln: Encuentro de Investigadores de la Región Noreste: Cerro Largo-Rivera-Tacuarembó, 1., 12 de agosto de 2016, Campus Interinstitucional de Tacuarembó, Tacuarembó. Libro de Resúmenes. Tacuarembó: UDELAR; INIA, 2016. p. 58| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Tacuarembó. |
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| 13. | | FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A....| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 15. | | RIVERO, R.; MINOLI, P.; PARODI, P.; MATTO, C.; ARMÚA-FERNÁNDEZ, M.T.; GIANNEECHINI, E.; CARVALHO, L.; VENZAL, J.M. Descripción de un foco de rangeliosis canina en el litoral noroeste del Uruguay. [Description of an outbreak of canine rangeliosis in the northwest littoral of Uruguay.]. Sección: Artículos originales. Veterinaria (Montevideo), 2017, vol. 54, no. 208, p. 15-22. -- OPEN ACCESS. Article history: Recibido 11 Octubre 2017, Aceptado 21 Noviembre 2017, Publicado 1 Diciembre 2017. -- Autor para correspondencia: rrivero@mgap.gub.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 16. | | PARODI, P.; ARMÚA-FERNÁNDEZ, M.T.; CORBELLINI, L.G.; RIVERO, R.; MIRABALLES, C.; RIET-CORREA, F.; VENZAL, J.M. Description of bovine babesiosis and anaplasmosis outbreaks in northern Uruguay between 2016 and 2018. Veterinary Parasitology: Regional Studies and Reports, 2022, Volume 29, Article number 100700. doi: https://doi.org/10.1016/j.vprsr.2022.100700 Article history: Received 7 April 2021; Received in revised form 27 November 2021; Accepted 26 January 2022; Available online 2 February 2022.
Corresponding author: Parodi, P.; Instituto Nacional de Investigación Agropecuaria,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 17. | | FÉLIX, M.L; ARMÚA-FERNÁNDEZ, M.T; PARODI, P.; BAZZANO , V.; MANGOLD, A.J.; VENZAL, J.M. Detection of a putative novel genotype of Anaplasma in gray-brocket deer (Mazama gouazoubira) from Uruguay. Experimental and Applied Acarology, Vol. 81, number 4, pag. 575-583, Aug 2020. Doi: https://doi.org/10.1007/s10493-020-00523-0 Article histort: Received 13 December 2019/Accepted03 July 2020/Published09 July 2020.We would like to thank Comisión Sectorial de Investigación Científica (CSIC), Programa Iniciación a la Investigación 2017, Project ID 160 for the...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 18. | | ANTÚNEZ, G.; CAJARVILLE, C.; FERNÁNDEZ, C.; DAYUTO, J.; ARTÚS, L; FERNÁNDEZ, M.; HORNOS, L.; CORREA, F.; BENTANCUR, O.; REPETTO, J. Estrategias de alimentación en la cría y recría de terneras lecheras y sus efectos sobre el crecimiento y la eficiencia de conservación. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 204-207.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 19. | | PARODI, P.; BAZZANO, V.; ARMÚA-FERNÁNDEZ, M.T.; FÉLIX, M.L.; CARVALHO, L.A.; FREIRE, J.; VENZAL, J.M. Molecular survey of Piroplasmida, Hepatozoon spp. and Anaplasmataceae in anemic and thrombocytopenic dogs from Uruguay. Original article. Veterinary Parasitology: Regional Studies and Reports. 2024, Volume 51, 101027. https://doi.org/10.1016/j.vprsr.2024.101027 Article history: Received 28 February 2024, Revised 8 April 2024, Accepted 17 April 2024, Available online 21 April 2024, Version of Record 23 April 2024. -- Corresponding author at: Rivera 1350, CP 50000 Salto, Uruguay. E-mail address:...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 20. | | UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 28 | |
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