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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
18/10/2017 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BOSCHI, F.; SCHVARTZMAN, C.; MURCHIO, S.; FERREIRA, V.; SIRI, M.; GALVÁN, G.; SMOKER, M.; STRANSFEL, L.; ZYPFEL, C.; VILARÓ, F.; DALLA RIZZA, M. |
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Afiliación : |
FEDERICO BOSCHI, INASE (Instituto Nacional de Semillas).; CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA FERREIRA, Universidad de la República (UdelaR)/ Facultad de Química; MARÍA SIRI, Universidad de la República (UdelaR)/ Facultad de Química; GUILLERMO GALVÁN, Universidad de la República (UdelaR)/ Facultad de Agronomía; MATTHEW SMOKER, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; LENA STRANSFEL, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; CYRIL ZYPFEL, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Enhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Frontiers in Plant Sciences, Volume 8, 25 September 2017, Article number 1642. OPEN ACCESS. |
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DOI : |
10.3389/fpls.2017.01642 |
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Idioma : |
Inglés |
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Notas : |
THIS ARTICLE IS PART OF THE RESEARCH TOPIC: Plant Pathogenic Ralstonia spp. From the Field to the Lab and Back Again: mechanisms of pathogen virulence and host resistance, population biology, community ecology and strategies for bacterial wilt disease management.
Article history: Received: 29 May 2017 / Accepted: 07 September 2017 / Published: 25 September 2017. |
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Contenido : |
ABSTRACT.
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.
© 2017 Boschi, Schvartzman, Murchio, Ferreira, Siri, Galván, Smoker, Stransfeld, Zipfel, Vilaró and Dalla-Rizza MenosABSTRACT.
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterol... Presentar Todo |
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Palabras claves : |
BACTERIAL WILT; EFR; PATTERN RECOGNITION RECEPTOR; POTATO; QUANTITATIVE RESISTANCE; RALSTONIA SOLANACEARUM. |
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Thesagro : |
PAPA; SOLANUM COMMERSONII; SOLANUM TUBEROSUM. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7375/1/Frontiers-in-Plants-2017-fpls-08-01642.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2017.01642/full
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Marc : |
LEADER 03254naa a2200373 a 4500
001 1057663
005 2019-10-15
008 2017 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2017.01642$2DOI
100 1 $aBOSCHI, F.
245 $aEnhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii.$h[electronic resource]
260 $c2017
500 $aTHIS ARTICLE IS PART OF THE RESEARCH TOPIC: Plant Pathogenic Ralstonia spp. From the Field to the Lab and Back Again: mechanisms of pathogen virulence and host resistance, population biology, community ecology and strategies for bacterial wilt disease management. Article history: Received: 29 May 2017 / Accepted: 07 September 2017 / Published: 25 September 2017.
520 $aABSTRACT. Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato. © 2017 Boschi, Schvartzman, Murchio, Ferreira, Siri, Galván, Smoker, Stransfeld, Zipfel, Vilaró and Dalla-Rizza
650 $aPAPA
650 $aSOLANUM COMMERSONII
650 $aSOLANUM TUBEROSUM
653 $aBACTERIAL WILT
653 $aEFR
653 $aPATTERN RECOGNITION RECEPTOR
653 $aPOTATO
653 $aQUANTITATIVE RESISTANCE
653 $aRALSTONIA SOLANACEARUM
700 1 $aSCHVARTZMAN, C.
700 1 $aMURCHIO, S.
700 1 $aFERREIRA, V.
700 1 $aSIRI, M.
700 1 $aGALVÁN, G.
700 1 $aSMOKER, M.
700 1 $aSTRANSFEL, L.
700 1 $aZYPFEL, C.
700 1 $aVILARÓ, F.
700 1 $aDALLA RIZZA, M.
773 $tFrontiers in Plant Sciences, Volume 8, 25 September 2017, Article number 1642. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
15/04/2021 |
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Actualizado : |
02/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTELLI, L.; BALBUENA, S.; BRANCHICCELA, B.; ZUNINO, P.; LIBERTI, J.; ENGEL, P.; ANTÚNEZ, K. |
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Afiliación : |
LORELEY CASTELLI, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE).; SOFÍA BALBUENA, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE).; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE).; JOANITO LIBERTI, Department of Fundamental Microbiology, University of Lausanne.; PHILIPP ENGEL, Department of Fundamental Microbiology, University of Lausanne.; KARINA ANTÚNEZ, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE). |
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Título : |
Impact of chronic exposure to sublethal doses of glyphosate on honey bee immunity, gut microbiota and infection by pathogens. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Microorganisms, April 2021, Volume 9; Issue 4, Article number 845. OPEN ACCESS. https://doi.org/10.3390/microorganisms9040845 |
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DOI : |
10.3390/microorganisms9040845 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 March 2021/Accepted: 31 March 2021/Published: 15 April 2021. |
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Contenido : |
Abstract: Glyphosate is the most used pesticide around the world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts on behavior, cognitive, sensory and developmental abilities, its use continues to grow. Recent studies have shown that it also alters the composition of the honey bee gut microbiota. In this study we explored the impact of chronic exposure to sublethal doses of glyphosate on the honey bee gut microbiota and its effects on the immune response, infection by Nosema ceranae and Deformed wing virus (DWV) and honey bee survival. Glyphosate combined with N. ceranae infection altered the structure and composition of the honey bee gut microbiota, for example by decreasing the relative abundance of the core members Snodgrassella alvi and Lactobacillus apis. Glyphosate increased the expression of some immune genes, possibly representing a physiological response to mitigate its negative effects. However, this response was not sufficient to maintain honey bee health, as glyphosate promoted the replication of DWV and decreased the expression of vitellogenin, which were accompanied by a reduced life span. Infection by N. ceranae also alters honey bee immunity although no synergistic effect with glyphosate was observed. These results corroborate previous findings suggesting deleterious effects of widespread use of glyphosate on honey bee health, and they contribute to elucidate the physiological mechanisms underlying a global decline of pollination services. MenosAbstract: Glyphosate is the most used pesticide around the world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts on behavior, cognitive, sensory and developmental abilities, its use continues to grow. Recent studies have shown that it also alters the composition of the honey bee gut microbiota. In this study we explored the impact of chronic exposure to sublethal doses of glyphosate on the honey bee gut microbiota and its effects on the immune response, infection by Nosema ceranae and Deformed wing virus (DWV) and honey bee survival. Glyphosate combined with N. ceranae infection altered the structure and composition of the honey bee gut microbiota, for example by decreasing the relative abundance of the core members Snodgrassella alvi and Lactobacillus apis. Glyphosate increased the expression of some immune genes, possibly representing a physiological response to mitigate its negative effects. However, this response was not sufficient to maintain honey bee health, as glyphosate promoted the replication of DWV and decreased the expression of vitellogenin, which were accompanied by a reduced life span. Infection by N. ceranae also alters honey bee immunity although no synergistic effect with glyphosate was observed. These results corroborate previous findings suggesting deleterious effects of widespread use of glyphosate on honey bee health, and they contribute to elucidate the physiological mechanisms underlying a g... Presentar Todo |
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Palabras claves : |
DEFORMED WING VIRUS; GLYPHOSATE; HONEY BEE GUT MICROBIOTA; HONEY BEE HEALTH; HONEY BEE IMMUNE RESPONSE; NOSEMA CERANAE; PESTICIDES. |
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Thesagro : |
ABEJAS; APICULTURA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16668/1/microorganisms-09-00845-v2.pdf
https://www.mdpi.com/2076-2607/9/4/845
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Marc : |
LEADER 02651naa a2200325 a 4500
001 1061984
005 2022-09-02
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/microorganisms9040845$2DOI
100 1 $aCASTELLI, L.
245 $aImpact of chronic exposure to sublethal doses of glyphosate on honey bee immunity, gut microbiota and infection by pathogens.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 16 March 2021/Accepted: 31 March 2021/Published: 15 April 2021.
520 $aAbstract: Glyphosate is the most used pesticide around the world. Although different studies have evidenced its negative effect on honey bees, including detrimental impacts on behavior, cognitive, sensory and developmental abilities, its use continues to grow. Recent studies have shown that it also alters the composition of the honey bee gut microbiota. In this study we explored the impact of chronic exposure to sublethal doses of glyphosate on the honey bee gut microbiota and its effects on the immune response, infection by Nosema ceranae and Deformed wing virus (DWV) and honey bee survival. Glyphosate combined with N. ceranae infection altered the structure and composition of the honey bee gut microbiota, for example by decreasing the relative abundance of the core members Snodgrassella alvi and Lactobacillus apis. Glyphosate increased the expression of some immune genes, possibly representing a physiological response to mitigate its negative effects. However, this response was not sufficient to maintain honey bee health, as glyphosate promoted the replication of DWV and decreased the expression of vitellogenin, which were accompanied by a reduced life span. Infection by N. ceranae also alters honey bee immunity although no synergistic effect with glyphosate was observed. These results corroborate previous findings suggesting deleterious effects of widespread use of glyphosate on honey bee health, and they contribute to elucidate the physiological mechanisms underlying a global decline of pollination services.
650 $aABEJAS
650 $aAPICULTURA
653 $aDEFORMED WING VIRUS
653 $aGLYPHOSATE
653 $aHONEY BEE GUT MICROBIOTA
653 $aHONEY BEE HEALTH
653 $aHONEY BEE IMMUNE RESPONSE
653 $aNOSEMA CERANAE
653 $aPESTICIDES
700 1 $aBALBUENA, S.
700 1 $aBRANCHICCELA, B.
700 1 $aZUNINO, P.
700 1 $aLIBERTI, J.
700 1 $aENGEL, P.
700 1 $aANTÚNEZ, K.
773 $tMicroorganisms, April 2021, Volume 9; Issue 4, Article number 845. OPEN ACCESS. https://doi.org/10.3390/microorganisms9040845
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